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Genetic Analysis of Mosquito Olfactory Preference for Humans

蚊子对人类嗅觉偏好的遗传分析

基本信息

批准号：
9118147
负责人：
Carolyn Sarah McBride
金额：
$ 24.47万
依托单位：
PRINCETON UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
2011
资助国家：
美国
起止时间：
2011-12-10 至 2017-08-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/9118147
关键词：
Aedes Affect Alleles Animals Anopheles gambiae Behavior Behavioral Biological Assay Brain Brain imaging Candidate Disease Gene Cataloging Catalogs Chemoreceptors Code Complementary DNA Culicidae Dengue Disease Employee Strikes Exhibits Female Genes Genetic Goals Human Human Bites Imaging Techniques Insect Bites Insect Vectors Insecta Lead Ligands Link Lobe Malaria Maxilla Mentors Methods Modality Molecular Morphology Mutation Neurons Odors Pattern Perception Peripheral Phase Physiology Population Positioning Attribute Process Research Sensory Signal Transduction Smell Perception Technology Testing Transgenic Organisms Validation Variant West Nile virus Work Yellow Fever base blind brain tissue comparative design forest genetic analysis human disease insight neurophysiology next generation novel strategies offspring preference prevent receptor relating to nervous system response transcriptome transcriptome sequencing vector mosquito zinc finger nuclease

项目摘要

DESCRIPTION (provided by applicant): The goal of this project is to understand the genetic mechanisms underlying the robust preference of the Dengue Fever Mosquito for human scent. The proposed research promises to (1) provide insight into the molecular basis of olfactory behavior and (2) provide specific information useful for preventing the spread of human disease by mosquitoes. The experimental plan leverages the power of a comparison between natural mosquito populations that exhibit a striking difference in olfactory preference. An ancestral, forest form of the Dengue Fever Mosquito prefers the odor of non-human animals, while a recently evolved, domestic form strongly prefers human odor. The first (mentored) part of the project will compare the antennal, maxillary palp, and brain transcriptomes of these two forms to generate a list of candidate genes that may regulate preference. The second (mentored) part of the project will compare odor-evoked neurophysiological activity in the first processing center of the brain to pinpoint differences in host odor representation and generate hypotheses about how the candidates act through olfactory signaling cascades to alter behavior. The third (independent) part of the project will use a combination of functional and transgenic studies to test the specific hypothesis that changes in the sequence and/or pattern of expression of candidate chemoreceptors and other olfactory genes are essential for human host preference. The results are expected to provide insight into the way olfactory preferences are determined and will focus efforts to develop novel approaches to prevent mosquitoes from biting humans and transmitting human disease.

描述（由申请人提供）：本项目的目标是了解登革热蚊子对人类气味强烈偏好的遗传机制。这项拟议中的研究有望（1）深入了解嗅觉行为的分子基础，（2）为预防蚊子传播人类疾病提供有用的具体信息。该实验计划利用了自然蚊子种群之间的比较，这些蚊子种群在嗅觉偏好方面表现出显着差异。一种原始的森林形式的登革热苔藓喜欢非人类动物的气味，而最近进化的一种家养形式强烈喜欢人类的气味。该项目的第一部分（指导）将比较这两种形式的触角，下颚须和大脑转录组，以生成可能调节偏好的候选基因列表。该项目的第二部分（指导）将比较大脑第一处理中心的气味诱发的神经生理活动，以确定宿主气味表征的差异，并生成关于候选人如何通过嗅觉信号级联改变行为的假设。该项目的第三（独立）部分将使用功能和转基因研究的组合来测试特定的假设，即候选化学感受器和其他嗅觉基因的序列和/或表达模式的变化对人类宿主偏好至关重要。这些结果有望为嗅觉偏好的确定方式提供深入了解，并将集中精力开发新的方法来防止蚊子叮咬人类和传播人类疾病。

项目成果

期刊论文数量（10）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Development of a pan-neuronal genetic driver in Aedes aegypti mosquitoes.

埃及伊蚊泛神经元遗传驱动的开发。

DOI：
10.1016/j.crmeth.2021.100042
发表时间：
2021
期刊：
Cell reports methods
影响因子：
0
作者：
Zhao,Zhilei;Tian,David;McBride,CarolynS
通讯作者：
McBride,CarolynS

Mosquito brains encode unique features of human odour to drive host seeking.

蚊子大脑编码人类气味的独特特征，以推动宿主寻求。

DOI：
10.1038/s41586-022-04675-4
发表时间：
2022-05
期刊：
Nature
影响因子：
64.8
作者：
通讯作者：

Origin and status of Culex pipiens mosquito ecotypes.

DOI：
10.1016/j.cub.2022.01.062
发表时间：
2022-03-14
期刊：
CURRENT BIOLOGY
影响因子：
9.2
作者：
Haba, Yuki;McBride, Lindy
通讯作者：
McBride, Lindy

Enhanced mosquito vectorial capacity underlies the Cape Verde Zika epidemic.

DOI：
10.1371/journal.pbio.3001864
发表时间：
2022-10
期刊：
PLOS BIOLOGY
影响因子：
9.8
作者：
Rose, Noah H.;Dabo, Stephanie;Leal, Silvania da Veiga;Sylla, Massamba;Diagne, Cheikh T.;Faye, Oumar;Faye, Ousmane;Sall, Amadou A.;McBride, Carolyn S.;Lambrechts, Louis
通讯作者：
Lambrechts, Louis

Exploring natural odour landscapes: A case study with implications for human-biting insects.

探索自然气味景观：对人类叮咬昆虫影响的案例研究。