Histology and Histomorphometry Core

组织学和组织形态计量学核心

• 批准号: 10024562
• 负责人: Patricia Florence Ducy
• 金额: $ 34.69万
• 依托单位: COLUMBIA UNIVERSITY HEALTH SCIENCES
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-09-15 至 2025-05-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10024562
• 关键词: Aging; Biological; Brain; Bromodeoxyuridine; Cell Count; Cells; Data; Detection; Equipment; Hematoxylin and Eosin Staining Method; Histologic; Histology; Human Resources; Image; In Situ Nick-End Labeling; Medical; Minerals; Mus; Osteogenesis; Pancreas; Paraffin; Research Personnel; Scanning; Skeleton; Specimen; Stains; Stress; Switzerland; System; Tissues; Tolonium chloride; Trichrome stain method; bone; cresyl violet; histological specimens; long bone; microCT; mouse model; programs; sensor; spine bone structure

The four projects of this Program Project heavily rely on the use of mouse models to demonstrate their hypotheses. The purpose of the Histology and Histomorphometry Core is therefore to consolidate equipment and expertise to provide the Investigators of these projects with state-of-the-art, quality controlled histologic and histomorphometric analyses of bone, pancreas and brain specimen as well as microCT of mouse bones. The Core contribution to this Program Project will be at 4 levels: 1. Processing mouse specimens for histology, immunolabeling and histomorphometry. Fixed and partially dehydrated mouse skeletons or pancreata will be embedded in plastic medium (vertebrae and long bones) or paraffin (pancreas). Fixed mouse brain specimen will either be sectioned without embedding or cryo- embedded and cryo-sectioned. 2. Performing specific stainings and immunostainings of mouse tissues sections. Classic histology stainings such as Hematoxylin/Eosin, Toluidine Blue, von Kossa/Van Gieson, Goldner trichrome, Cresyl violet as well as more specific reactive ones such as TUNEL and TRAP stainings will be performed by the Core personnel at the request of investigators. The Core will also perform immunodetection studies using fluorescent and enzymatic detection on sections. 3. Performing histomorphometric analyses of mouse bone, pancreas and brain specimens. The OsteoMeasure Histomorphometry System (OsteoMetrics Inc., Decatur, GA) will be used to assess static parameters, such as bone volume, trabecular number and cell counts, and dynamic parameters of bone formation, such as mineral apposition rates, from non-decalcified bone sections. It will also be used to quantify cell mass/proliferation in pancreas and TUNEL or BrdU positive cells in brain sections. Image J will be used for all other quantifications. The Core will provide investigators with histomorphometric data and will help them in evaluating their biological relevance. 4. Analyzing mouse bone specimen by microCT. Fixed and partially dehydrated mouse long bones and vertebrae will be provided to the Core by project investigators. These specimens will then be scanned using a VivaCT 40 microCT (Scanco Medical, Switzerland).

本计划的四个项目严重依赖于使用小鼠模型来证明其 假设因此，组织学和组织形态计量学核心的目的是巩固设备 和专业知识，为这些项目的研究者提供最先进的、质量受控的组织学检查。 骨、胰腺和脑标本的组织形态计量学分析以及小鼠骨的microCT。 对该计划项目的核心贡献将分为4个级别： 1.处理用于组织学、免疫标记和组织形态计量学的小鼠标本。固定和部分 脱水小鼠骨骼或胰腺将嵌入塑料介质（椎骨和长骨）中 或石蜡（胰腺）。固定的小鼠脑标本将在不包埋或冷冻的情况下切片。 包埋并冷冻切片。 2.对小鼠组织切片进行特异性染色和免疫染色。经典组织学染色 例如苏木精/曙红、甲苯胺蓝、vonKossa/货车Gieson、Goldner三色、甲酚紫 以及更特异的反应性染色，如TUNEL和TRAP染色，将由核心细胞进行。 应调查人员的要求。核心还将使用以下方法进行免疫检测研究： 切片上的荧光和酶检测。 3.对小鼠骨骼、胰腺和脑标本进行组织形态计量学分析。的 OsteoMeasure组织形态测量系统（OsteoMeasure Inc.，Decatur，GA）将用于评估静态 参数，如骨体积、骨小梁数量和细胞计数，以及骨的动态参数 非脱钙骨切片中的矿物质沉积率等。它还将用于 定量胰腺中的细胞团/增殖和脑切片中的TUNEL或BrdU阳性细胞。图像J将 用于所有其他量化。核心将为研究者提供组织形态学数据， 将帮助他们评估其生物学意义。 4.小鼠骨标本显微CT分析。固定和部分脱水的小鼠长骨， 椎骨将由项目研究者提供给核心。这些标本将被扫描 使用VivaCT 40 microCT（Switzerland，Switzerland）。