CSHL X-Ray Methods in Structural Biology

结构生物学中的 CSHL X 射线方法

• 批准号: 9135498
• 负责人: TERRI I. GRODZICKER
• 金额: $ 8.15万
• 依托单位: COLD SPRING HARBOR LABORATORY
• 依托单位国家: 美国
• 财政年份: 2012
• 资助国家: 美国
• 起止时间: 2012-09-01 至 2017-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9135498
• 关键词: Area; Biochemistry; Biological; Cancer Biology; Complex; Course Content; Crystallization; Crystallography; Data; Data Collection; Deposition; Development; Discipline; Disease; Doctor of Medicine; Doctor of Philosophy; Drug Design; Educational process of instructing; Ensure; Faculty; Freezing; Funding; Industry; Knowledge; Laboratories; Learning; Left; Light; Malignant Neoplasms; Maps; Medical Students; Methods; Molecular; New York; Nucleic Acids; Optics; Participant; Pharmaceutical Preparations; Postdoctoral Fellow; Process; Proteins; Research; Research Personnel; Roentgen Rays; Running; Scientist; Series; Solvents; Source; Structure; Students; Synchrotrons; Techniques; Training; Tumor Suppressor Genes; United States; Universities; Update; Validation; Virus; Work; X ray diffraction analysis; X-Ray Crystallography; X-Ray Diffraction; anticancer research; arm; base; biological research; data reduction; design; electron density; experience; falls; graduate student; instructor; laboratory experience; lecturer; lectures; macromolecule; medical schools; method development; programs; protein function; protein structure; protein structure function; research study; structural biology; student training; theories; three dimensional structure; translational approach; tumor; work-study

DESCRIPTION (provided by applicant): Cold Spring Harbor Laboratory proposes to continue the course on X-Ray Methods in Structural Biology to be held in the fall of 2012-2016. Crystallography and X-ray diffraction yield a wealth of structural information unobtainable through other methods. This intensive 16-day laboratory/computational course focuses on the major X-ray crystallographic techniques used to determine the three-dimensional structures of macromolecules. The course provides the opportunity for research workers in different biological disciplines to become grounded in the techniques and principles of macromolecular crystallography. It is designed for scientists with a working knowledge of protein structure and function, but who are new to macromolecular crystallography. Topics to be covered include basic diffraction theory, crystallization (proteins, nucleic acids, viruses and complexes), crystal characterization, X-ray sources and optics, synchrotrons, crystal freezing, data collection, X-ray data reduction, multiple isomorphous replacement, multiwavelength/single wavelength anomalous diffraction, molecular replacement, solvent flattening, non-crystallographic symmetry averaging, electron density map interpretation, molecular graphics, structure refinement, structure validation, coordinate deposition, structure presentation along with other relevant topics. The lecturers are chosen on the basis of their contributions to, and knowledge of, the areas covered in the course. In addition, several of the lecturers present their current research to illustrate the principles that are taught in the course. Participants learn through extensive hands-on experiments where they crystallize and determine protein structures, along with lectures and informal discussions on the theory behind the techniques from world leaders in methods development. The course day starts in the morning and runs throughout the day until late at night. There is also a trip to the National Synchrotron Light Source facility at Brookhaven National Laboratory to collect multiwavelength X-ray diffraction data. Applications are open to a wide range of students including advanced graduate students, medical students, post-doctoral fellows, faculty at universities and medical schools, as well as Ph.D. and M.D. scientists from industry. The course is designed to allow students to learn techniques that can be immediately incorporated into their own research. This enables the training of new researchers who can not only solve structures relevant to their field of biological endeavor but also contribute to translational approaches including rational drug design.

描述（由申请人提供）：冷泉港实验室建议继续在2012-2016年秋季举行的结构生物学X射线方法课程。晶体学和X射线衍射提供了通过其他方法无法获得的丰富结构信息。这个为期16天的实验室/计算密集课程侧重于用于确定大分子三维结构的主要X射线晶体学技术。该课程为不同生物学科的研究人员提供了在大分子晶体学的技术和原理中扎根的机会。它是为具有蛋白质结构和功能工作知识但对大分子晶体学不熟悉的科学家设计的。课程内容包括基本衍射理论、结晶（蛋白质、核酸、病毒和复合物）、晶体 表征、X射线源和光学、同步加速器、晶体冷冻、数据收集、X射线数据简化、多个同晶置换、多波长/单波长异常衍射、分子置换、溶剂平坦化、非晶体对称平均、电子密度图解释、分子图形学、结构精修、结构验证、坐标沉积、结构呈现沿着以及其他相关主题。讲师是根据他们对课程所涵盖领域的贡献和知识来选择的。此外，几位讲师介绍了他们目前的研究，以说明在课程中教授的原则。参与者通过广泛的动手实验进行学习，在这些实验中结晶并确定蛋白质结构，同时沿着世界方法开发领导者关于技术背后理论的讲座和非正式讨论。课程从早上开始，一整天都在运行，直到深夜。还有一次前往布鲁克海文的国家同步加速器光源设施 国家实验室收集多波长X射线衍射数据。申请是开放给广泛的学生，包括高级研究生，医学生，博士后研究员，教师在大学和医学院，以及博士。和医学博士。工业界的科学家。该课程旨在让学生学习可以立即纳入自己的研究技术。这使得新的研究人员不仅可以解决与其生物奋进领域相关的结构，还可以为包括合理药物设计在内的转化方法做出贡献。