CSHL X-Ray Methods in Structural Biology

简介(申请人提供)：冷泉港实验室拟于2012-2016年度秋季继续举办结构生物学X射线方法课程。结晶学和X射线衍射产生了通过其他方法无法获得的丰富的结构信息。这门为期16天的密集实验室/计算课程侧重于用于确定大分子三维结构的主要X射线结晶学技术。这门课程为不同生物学科的研究人员提供了在高分子结晶学的技术和原理方面扎根的机会。它是为对蛋白质结构和功能有实际工作知识的科学家设计的，但对大分子结晶学来说是新手。课程包括基本绕射理论、结晶(蛋白质、核酸、病毒和复合体)、晶体 X射线表征、X射线源与光学、同步加速器、晶体冻结、数据收集、X射线数据还原、多重同晶置换、多波长/单波长异常衍射、分子置换、溶剂扁平化、非晶体对称性平均、电子密度图解释、分子图解、结构精炼、结构验证、配位沉积、结构呈现以及其他相关课题。讲师是根据他们对课程所涵盖领域的贡献和知识来选择的。此外，几位讲师介绍了他们目前的研究，以说明课程中教授的原则。参与者通过广泛的动手实验学习，他们在那里结晶和确定蛋白质结构，以及世界各国方法开发领导人关于技术背后的理论的讲座和非正式讨论。白天的课程从早上开始，一直持续到深夜。此外，还将参观布鲁克海文的国家同步加速器光源设施 国家实验室收集多波长X射线衍射数据。申请范围广泛，包括高级研究生、医学生、博士后研究员、大学和医学院的教职员工，以及工业界的博士和医学博士科学家。这门课程旨在让学生学习可以立即融入他们自己的研究中的技术。这使得能够培训新的研究人员，他们不仅能够解决与他们的生物努力领域相关的结构，而且还有助于包括合理药物设计在内的翻译方法。

DESCRIPTION (provided by applicant): Cold Spring Harbor Laboratory proposes to continue the course on X-Ray Methods in Structural Biology to be held in the fall of 2012-2016. Crystallography and X-ray diffraction yield a wealth of structural information unobtainable through other methods. This intensive 16-day laboratory/computational course focuses on the major X-ray crystallographic techniques used to determine the three-dimensional structures of macromolecules. The course provides the opportunity for research workers in different biological disciplines to become grounded in the techniques and principles of macromolecular crystallography. It is designed for scientists with a working knowledge of protein structure and function, but who are new to macromolecular crystallography. Topics to be covered include basic diffraction theory, crystallization (proteins, nucleic acids, viruses and complexes), crystal characterization, X-ray sources and optics, synchrotrons, crystal freezing, data collection, X-ray data reduction, multiple isomorphous replacement, multiwavelength/single wavelength anomalous diffraction, molecular replacement, solvent flattening, non-crystallographic symmetry averaging, electron density map interpretation, molecular graphics, structure refinement, structure validation, coordinate deposition, structure presentation along with other relevant topics. The lecturers are chosen on the basis of their contributions to, and knowledge of, the areas covered in the course. In addition, several of the lecturers present their current research to illustrate the principles that are taught in the course. Participants learn through extensive hands-on experiments where they crystallize and determine protein structures, along with lectures and informal discussions on the theory behind the techniques from world leaders in methods development. The course day starts in the morning and runs throughout the day until late at night. There is also a trip to the National Synchrotron Light Source facility at Brookhaven National Laboratory to collect multiwavelength X-ray diffraction data. Applications are open to a wide range of students including advanced graduate students, medical students, post-doctoral fellows, faculty at universities and medical schools, as well as Ph.D. and M.D. scientists from industry. The course is designed to allow students to learn techniques that can be immediately incorporated into their own research. This enables the training of new researchers who can not only solve structures relevant to their field of biological endeavor but also contribute to translational approaches including rational drug design.