CSHL X-Ray Methods in Structural Biology Course

CSHL 结构生物学 X 射线方法课程

• 批准号: 9767812
• 负责人: TERRI I. GRODZICKER
• 金额: $ 9.07万
• 依托单位: COLD SPRING HARBOR LABORATORY
• 依托单位国家: 美国
• 财政年份: 2012
• 资助国家: 美国
• 起止时间: 2012-09-01 至 2023-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9767812
• 关键词: Academia; Area; Automation; Awareness; Biochemistry; Biological; Complement; Complex; Computing Methodologies; Course Content; Cryoelectron Microscopy; Crystallization; Crystallography; Data Collection; Deposition; Development; Discipline; Disease; Disease Progression; Doctor of Medicine; Doctor of Philosophy; Drug Design; Ensure; Faculty; Funding; G-Protein-Coupled Receptors; Generations; Grant; Hand; Harvest; Home environment; Immersion Investigative Technique; Industry; Infrastructure; Inherited; Knowledge; Laboratories; Learning; Lipids; Macromolecular Complexes; Malignant Neoplasms; Maps; Medical Students; Membrane Proteins; Methodology; Methods; Modeling; Modification; Molecular; Molecular Structure; New York; Nobel Prize; Nucleic Acids; Onset of illness; Optics; Participant; Pharmaceutical Preparations; Phase; Postdoctoral Fellow; Procedures; Process; Research; Research Personnel; Resolution; Roentgen Rays; Running; Scientist; Series; Source; Structure; Students; Synchrotrons; Talents; Techniques; Therapeutic antibodies; Time; Training; United States; Universities; Update; Vaccines; Validation; Virus; Work; X ray diffraction analysis; X-Ray Crystallography; base; biological research; computerized data processing; density; design; electron density; experience; experimental study; falls; graduate student; instructor; lecturer; lectures; macromolecule; medical schools; method development; model building; programs; protein structure function; small molecule; structural biology; theories; translational approach; work-study; x-ray free-electron laser

ABSTRACT Cold Spring Harbor Laboratory proposes to continue the course on X-Ray Methods in Structural Biology to be held in the fall of 2017-2021. X-ray methods, spearheaded by X-ray crystallography, yield a wealth of structural information unobtainable through other methods. This intensive 16-day course combines a thorough theoretical approach with practical experience, including extensive laboratory and computational training. Lectures and practical sessions focus on X-ray crystallographic techniques, which remain indispensable in yielding atomic level structural detail of macromolecules, but also extend to X-ray solution scattering (SAXS), and serial crystallography in X-ray free electron lasers (XFEL) and synchrotron sources. The course aspires to provide the opportunity for researchers working in different biological disciplines to become grounded in the techniques and principles of macromolecular crystallography and complementary methods. Learning the theory and practice of the methods at hand, allows participants with a working knowledge of macromolecular structure and function, but who are new to X-ray methods, to harvest the power of recent developments in automation and apply the methods to their current research questions. Topics in X-ray crystallography include basic diffraction theory, crystallization of soluble and also membrane proteins (including lipidic cubic phase techniques), nucleic acids, macromolecular complexes and viruses, crystal cryo-protection and characterization, home, synchrotron, XFEL X-ray sources and optics, X-ray diffraction data collection and data processing, experimental phasing methods including anomalous diffraction, molecular replacement, density modification, electron density map interpretation and model building, structure refinement and validation, as well as coordinate deposition and structure presentation. Topics in X-ray scattering include theory lectures and computational training to derive SAXS invariants and models to understand macromolecular domain organization. Following three decades that have seen two Nobel prize winners graduating from the course, it is time for change: within the duration of the grant, the organization and running of the course will be handed off to a new generation. Two new instructors will participate in the team from the start, and by the end of the grant period a new set of instructors will ensure continuity and renewal. The course will continue to teach participants through extensive hands-on experiments along with lectures and informal discussions on the theory behind the techniques and the latest developments in automating experimental and computational procedures, from world leaders in methods development. Applications are open to a wide range of students including advanced graduate students, medical students, post-doctoral fellows, faculty at universities and medical schools, as well as Ph.D. and M.D. scientists from industry, aiming to allow students to learn techniques that can be immediately incorporated into their own research, also enabling translational approaches including rational drug design.

抽象的 冷春港实验室建议继续有关结构性X射线方法的课程 生物学将于2017 - 2021年秋季举行。 X射线方法，由X射线率领 晶体学，通过其他方法产生大量的结构信息。 这个密集的16天课程将彻底的理论方法与实用 经验，包括广泛的实验室和计算培训。讲座和实用 会议的重点是X射线晶体学技术，这些技术在屈服方面仍然必不可少 大分子的原子水平结构细节，但也延伸至X射线溶液散射 （SAX）和X射线免费电子激光器（XFEL）和Synchrotron中的串行晶体学 来源。该课程渴望为从事不同的研究人员提供机会 生物学学科以大分子的技术和原理为基础 晶体学和互补方法。学习方法的理论和实践 在手头，允许参与者具有大分子结构和功能的工作知识， 但是谁是X射线方法的新手，以收获最新自动化发展的力量 并将方法应用于他们当前的研究问题。 X射线晶体学的主题 包括基本衍射理论，可溶性和膜蛋白的结晶（包括 脂质立方相技术），核酸，大分子复合物和病毒，晶体 冷冻保护和表征，家庭，同步器，XFEL X射线源和光学器件，X射线 衍射数据收集和数据处理，包括 异常衍射，分子置换，密度修饰，电子密度图 解释和模型构建，结构改进和验证以及坐标 沉积和结构表现。 X射线散射的主题包括理论讲座和 计算训练以推导萨克斯群体不变性和模型以了解大分子 域组织。 在三十年之后，这两名诺贝尔奖获得者从该课程毕业了 是改变的时间：在赠款的持续时间内，课程的组织和运行 将交给新一代。两名新教练将参加团队 开始，到赠款期结束时，一组新的讲师将确保连续性和 更新。该课程将继续通过大量的动手实验来教参与者 以及关于技术背后理论和最新理论的讲座和非正式讨论 自动化实验和计算程序的发展，来自世界领导者 方法开发。申请向包括高级学生在内的众多学生开放 研究生，医学生，博士后研究员，大学的教职员工和医学 学校以及博士学位和工业的医学博士科学家，旨在允许学生学习 可以立即纳入自己的研究的技术，也可以实现 翻译方法，包括理性药物设计。