CSHL X-Ray Methods in Structural Biology Course

CSHL 结构生物学 X 射线方法课程

• 批准号: 9767812
• 负责人: TERRI I. GRODZICKER
• 金额: $ 9.07万
• 依托单位: COLD SPRING HARBOR LABORATORY
• 依托单位国家: 美国
• 财政年份: 2012
• 资助国家: 美国
• 起止时间: 2012-09-01 至 2023-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9767812
• 关键词: Academia; Area; Automation; Awareness; Biochemistry; Biological; Complement; Complex; Computing Methodologies; Course Content; Cryoelectron Microscopy; Crystallization; Crystallography; Data Collection; Deposition; Development; Discipline; Disease; Disease Progression; Doctor of Medicine; Doctor of Philosophy; Drug Design; Ensure; Faculty; Funding; G-Protein-Coupled Receptors; Generations; Grant; Hand; Harvest; Home environment; Immersion Investigative Technique; Industry; Infrastructure; Inherited; Knowledge; Laboratories; Learning; Lipids; Macromolecular Complexes; Malignant Neoplasms; Maps; Medical Students; Membrane Proteins; Methodology; Methods; Modeling; Modification; Molecular; Molecular Structure; New York; Nobel Prize; Nucleic Acids; Onset of illness; Optics; Participant; Pharmaceutical Preparations; Phase; Postdoctoral Fellow; Procedures; Process; Research; Research Personnel; Resolution; Roentgen Rays; Running; Scientist; Series; Source; Structure; Students; Synchrotrons; Talents; Techniques; Therapeutic antibodies; Time; Training; United States; Universities; Update; Vaccines; Validation; Virus; Work; X ray diffraction analysis; X-Ray Crystallography; base; biological research; computerized data processing; density; design; electron density; experience; experimental study; falls; graduate student; instructor; lecturer; lectures; macromolecule; medical schools; method development; model building; programs; protein structure function; small molecule; structural biology; theories; translational approach; work-study; x-ray free-electron laser

ABSTRACT Cold Spring Harbor Laboratory proposes to continue the course on X-Ray Methods in Structural Biology to be held in the fall of 2017-2021. X-ray methods, spearheaded by X-ray crystallography, yield a wealth of structural information unobtainable through other methods. This intensive 16-day course combines a thorough theoretical approach with practical experience, including extensive laboratory and computational training. Lectures and practical sessions focus on X-ray crystallographic techniques, which remain indispensable in yielding atomic level structural detail of macromolecules, but also extend to X-ray solution scattering (SAXS), and serial crystallography in X-ray free electron lasers (XFEL) and synchrotron sources. The course aspires to provide the opportunity for researchers working in different biological disciplines to become grounded in the techniques and principles of macromolecular crystallography and complementary methods. Learning the theory and practice of the methods at hand, allows participants with a working knowledge of macromolecular structure and function, but who are new to X-ray methods, to harvest the power of recent developments in automation and apply the methods to their current research questions. Topics in X-ray crystallography include basic diffraction theory, crystallization of soluble and also membrane proteins (including lipidic cubic phase techniques), nucleic acids, macromolecular complexes and viruses, crystal cryo-protection and characterization, home, synchrotron, XFEL X-ray sources and optics, X-ray diffraction data collection and data processing, experimental phasing methods including anomalous diffraction, molecular replacement, density modification, electron density map interpretation and model building, structure refinement and validation, as well as coordinate deposition and structure presentation. Topics in X-ray scattering include theory lectures and computational training to derive SAXS invariants and models to understand macromolecular domain organization. Following three decades that have seen two Nobel prize winners graduating from the course, it is time for change: within the duration of the grant, the organization and running of the course will be handed off to a new generation. Two new instructors will participate in the team from the start, and by the end of the grant period a new set of instructors will ensure continuity and renewal. The course will continue to teach participants through extensive hands-on experiments along with lectures and informal discussions on the theory behind the techniques and the latest developments in automating experimental and computational procedures, from world leaders in methods development. Applications are open to a wide range of students including advanced graduate students, medical students, post-doctoral fellows, faculty at universities and medical schools, as well as Ph.D. and M.D. scientists from industry, aiming to allow students to learn techniques that can be immediately incorporated into their own research, also enabling translational approaches including rational drug design.

摘要 冷泉港实验室建议继续进行结构中的X射线方法课程 生物学将于2017-2021年秋季举行。X射线方法，以X射线 晶体学，产生丰富的结构信息无法通过其他方法获得。 这门为期16天的密集课程将彻底的理论方法与实践相结合 经验，包括广泛的实验室和计算培训。讲座和实践 会议的重点是X射线晶体学技术，这仍然是必不可少的生产 大分子的原子水平结构细节，而且还扩展到X射线溶液散射 （SAXS）和X射线自由电子激光（XFEL）和同步加速器中的系列晶体学 源该课程旨在为在不同领域工作的研究人员提供机会， 生物学科将以大分子生物学的技术和原理为基础， 晶体学和互补方法。学习方法的理论和实践 在手，允许参与者与大分子结构和功能的工作知识， 但他们是X射线方法的新手，他们收获了自动化最新发展的力量， 并将这些方法应用于他们当前的研究问题。X射线晶体学专题 包括基本衍射理论、可溶性和膜蛋白结晶（包括 立方相技术），核酸，大分子复合物和病毒，晶体 低温保护和表征，家用，同步加速器，XFEL X射线源和光学，X射线 衍射数据收集和数据处理，实验定相方法，包括 反常衍射，分子置换，密度修正，电子密度图 解释和模型建立，结构细化和验证，以及协调 沉积和构造表现。X射线散射的主题包括理论讲座和 计算训练，以获得SAXS不变量和模型，以了解大分子 域组织。 三十年来，两名诺贝尔奖获得者从该课程毕业， 是时候改变了：在赠款的期限内，课程的组织和运行 将被传递给下一代两名新教练将加入该团队 开始，并在赠款期结束时，一套新的导师将确保连续性， 退款该课程将继续通过广泛的动手实验来教授参与者 沿着讲座和非正式的讨论背后的技术和最新的理论 在自动化实验和计算程序的发展，从世界领导人， 方法开发申请是开放给广泛的学生，包括先进的 研究生、医学生、博士后研究员、大学和医学院教职员工 学校，以及博士学位。和医学博士来自工业界的科学家，旨在让学生学习 技术，可以立即纳入自己的研究，也使 包括合理药物设计在内的转化方法。