CSHL X-Ray Methods in Structural Biology Course

CSHL 结构生物学 X 射线方法课程

• 批准号: 9767812
• 负责人: TERRI I. GRODZICKER
• 金额: $ 9.07万
• 依托单位: COLD SPRING HARBOR LABORATORY
• 依托单位国家: 美国
• 财政年份: 2012
• 资助国家: 美国
• 起止时间: 2012-09-01 至 2023-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9767812
• 关键词: Academia; Area; Automation; Awareness; Biochemistry; Biological; Complement; Complex; Computing Methodologies; Course Content; Cryoelectron Microscopy; Crystallization; Crystallography; Data Collection; Deposition; Development; Discipline; Disease; Disease Progression; Doctor of Medicine; Doctor of Philosophy; Drug Design; Ensure; Faculty; Funding; G-Protein-Coupled Receptors; Generations; Grant; Hand; Harvest; Home environment; Immersion Investigative Technique; Industry; Infrastructure; Inherited; Knowledge; Laboratories; Learning; Lipids; Macromolecular Complexes; Malignant Neoplasms; Maps; Medical Students; Membrane Proteins; Methodology; Methods; Modeling; Modification; Molecular; Molecular Structure; New York; Nobel Prize; Nucleic Acids; Onset of illness; Optics; Participant; Pharmaceutical Preparations; Phase; Postdoctoral Fellow; Procedures; Process; Research; Research Personnel; Resolution; Roentgen Rays; Running; Scientist; Series; Source; Structure; Students; Synchrotrons; Talents; Techniques; Therapeutic antibodies; Time; Training; United States; Universities; Update; Vaccines; Validation; Virus; Work; X ray diffraction analysis; X-Ray Crystallography; base; biological research; computerized data processing; density; design; electron density; experience; experimental study; falls; graduate student; instructor; lecturer; lectures; macromolecule; medical schools; method development; model building; programs; protein structure function; small molecule; structural biology; theories; translational approach; work-study; x-ray free-electron laser

ABSTRACT Cold Spring Harbor Laboratory proposes to continue the course on X-Ray Methods in Structural Biology to be held in the fall of 2017-2021. X-ray methods, spearheaded by X-ray crystallography, yield a wealth of structural information unobtainable through other methods. This intensive 16-day course combines a thorough theoretical approach with practical experience, including extensive laboratory and computational training. Lectures and practical sessions focus on X-ray crystallographic techniques, which remain indispensable in yielding atomic level structural detail of macromolecules, but also extend to X-ray solution scattering (SAXS), and serial crystallography in X-ray free electron lasers (XFEL) and synchrotron sources. The course aspires to provide the opportunity for researchers working in different biological disciplines to become grounded in the techniques and principles of macromolecular crystallography and complementary methods. Learning the theory and practice of the methods at hand, allows participants with a working knowledge of macromolecular structure and function, but who are new to X-ray methods, to harvest the power of recent developments in automation and apply the methods to their current research questions. Topics in X-ray crystallography include basic diffraction theory, crystallization of soluble and also membrane proteins (including lipidic cubic phase techniques), nucleic acids, macromolecular complexes and viruses, crystal cryo-protection and characterization, home, synchrotron, XFEL X-ray sources and optics, X-ray diffraction data collection and data processing, experimental phasing methods including anomalous diffraction, molecular replacement, density modification, electron density map interpretation and model building, structure refinement and validation, as well as coordinate deposition and structure presentation. Topics in X-ray scattering include theory lectures and computational training to derive SAXS invariants and models to understand macromolecular domain organization. Following three decades that have seen two Nobel prize winners graduating from the course, it is time for change: within the duration of the grant, the organization and running of the course will be handed off to a new generation. Two new instructors will participate in the team from the start, and by the end of the grant period a new set of instructors will ensure continuity and renewal. The course will continue to teach participants through extensive hands-on experiments along with lectures and informal discussions on the theory behind the techniques and the latest developments in automating experimental and computational procedures, from world leaders in methods development. Applications are open to a wide range of students including advanced graduate students, medical students, post-doctoral fellows, faculty at universities and medical schools, as well as Ph.D. and M.D. scientists from industry, aiming to allow students to learn techniques that can be immediately incorporated into their own research, also enabling translational approaches including rational drug design.

摘要 冷泉港实验室建议继续X射线方法在结构中的课程 生物学将于2017-2021年秋季举行。以X射线为先导的X射线方法 结晶学，产生了其他方法无法获得的丰富的结构信息。 这门为期16天的强化课程将透彻的理论方法与实践相结合。 经验，包括广泛的实验室和计算培训。讲授和实践 会议的重点是X射线结晶学技术，这些技术在生产过程中仍然不可或缺 大分子的原子级结构细节，还延伸到X射线溶液散射 (SAXS)，以及X射线自由电子激光(XFEL)和同步加速器中的系列结晶学 消息来源。该课程致力于为在不同领域工作的研究人员提供机会 生物学科将以大分子的技术和原理为基础 结晶学和补充方法。学习方法的理论和实践 在手边，允许参与者具有大分子结构和功能的实用知识， 但谁是X射线方法的新手，才能从自动化的最新发展中获得力量 并将这些方法应用于他们目前的研究问题。X射线结晶学专题 包括基本衍射理论、可溶性和膜蛋白的结晶(包括 脂质立方相技术)、核酸、大分子络合物和病毒、晶体 低温防护和表征，家用，同步加速器，XFEL X射线源和光学，X射线 衍射数据采集和数据处理，实验定相方法包括 反常衍射、分子置换、密度修正、电子密度图 解释和模型建立、结构改进和验证以及协调 沉积和结构展示。X射线散射的主题包括理论讲座和 计算培训以获得理解大分子的SAXS不变量和模型 域组织。 在经历了30年的时间里，两位诺贝尔奖获得者从这门课程中毕业，它 是时候改变了：在赠款期限内，课程的组织和运行 将被移交给新一代。两名新的教官将从 开始，在授权期结束时，一批新的教官将确保连续性和 更新。课程将继续通过广泛的动手实验来教授参与者 以及关于技术背后的理论和最新技术的讲座和非正式讨论 在自动化实验和计算过程方面的进展，来自世界领先的 方法发展。申请范围很广，包括高级 研究生、医学生、博士后研究员、大学和医学院的教职员工 学校，以及来自工业界的博士和医学博士科学家，旨在让学生学习 这些技术可以立即并入他们自己的研究中，也使 包括合理药物设计在内的转化方法。