Scanning Blockface EM of Murine and Human Taste Buds

扫描小鼠和人类味蕾的块面电镜

• 批准号: 9088450
• 负责人: Thomas E Finger
• 金额: $ 32.98万
• 依托单位: UNIVERSITY OF COLORADO DENVER
• 依托单位国家: 美国
• 财政年份: 2015
• 资助国家: 美国
• 起止时间: 2015-07-01 至 2020-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9088450
• 关键词: 3-Dimensional; Anatomy; Architecture; Brain; Cell Communication; Cells; Code; Complex; Data; Databases; Detection; Elements; Exhibits; Face; Funding; Goals; Human; Image; Imagery; Individual; Investigation; Knowledge; Label; Lead; Mitochondria; Molecular; Morphology; Mus; Nerve; Nerve Fibers; Neurotransmitters; Nutrient; Nutritional; Pattern; Peripheral; Peripheral Nervous System; Physiological; Property; Receptor Cell; Receptor Signaling; Reporting; Research Design; Research Personnel; Retinal; Rodent; Scanning; Sensory; Signal Transduction; Site; Source; Specificity; Structure; Synapses; System; Taste Buds; Taste Perception; Technology; Testing; Time; Toxin; Type I Epithelial Receptor Cell; Type II Epithelial Receptor Cell; Visual; afferent nerve; cell type; connectome; detector; information processing; light microscopy; new technology; novel; receptor; reconstruction; relating to nervous system; response; taste system; taste transduction; theories; transmission process; vesicular release

 DESCRIPTION (provided by applicant): Taste buds are complex multicellular endorgans essential for detection of taste. The cells of a taste bud distinguish between the nutritious and the potentially toxic, and communicate this information to the taste nerves innervating them. Despite recent advances in understanding the molecular signaling underlying taste transduction, a complete understanding of cell-cell interactions and transmission of information to the peripheral nervous system is lacking. We propose to use a newly invented technology, serial blockface scanning EM, to generate a complete 3D picture of taste bud structure from both mice and humans in order to test whether taste information is transmitted along dedicated "labeled lines" dedicated to an individual taste quality, or whether the brain receives a more complex signal from the taste buds. The novel EM technology will permit detailed 3D reconstruction of the taste bud connectome allowing for analysis of key features including: 1) whether individual nerve fibers make functional contact with more than one cell type in a bud, and 2) whether the glial-like Type I cells fully separate the other cell types or whether there is potential for functonal interactions between receptor cells encoding the different taste qualities. This greater understanding of the detailed structure of taste buds will help investigators formulate data-based hypotheses regarding functional interactions between the different cellular elements and a more clear understanding of how taste information is transmitted from taste buds to brain. RELEVANCE: Taste buds are complex sensory endorgans crucial for detection of both nutrients and toxins. While many of the molecular mechanisms underlying taste have been elucidated during the last decade, we still do not understand the functional organization and interrelationships between the diverse cells of a taste bud. Investigations in this proposal will rely on new technology, serial blockface EM, to generate detailed 3- dimensional images of taste buds to resolve questions about neural connectivity and cell-cell interactions in transmission of taste information from taste bud to brain.

 描述（由适用提供）：味蕾是对检测味道必不可少的复杂多细胞内膜。味蕾的细胞区分营养和潜在的有毒物质，并将这些信息传达给味觉神经。尽管最近了解味道传播的分子信号传导方面取得了进步，但仍缺乏对细胞细胞相互作用和信息传播到周围神经系统的完全理解。我们建议使用新发明的技术，即串行块扫描EM，以产生来自小鼠和人类味蕾结构的完整的3D图片，以测试味觉信息是否沿专用的“标记线”传输到专门的“标签线”，或者是从个人味道质量的专用“标签线”，或者是否从味蕾中接收到更复杂的脑部信号。新颖的EM技术将允许对味蕾连接组的详细3D重建，以分析关键特征，包括：1）单个神经纤维是否与芽中的多种细胞类型进行功能接触，以及2）2）是否完全分离出其他细胞类型的胶质样类型的细胞，或者是否有能力在构造不同味道质量的受体相互作用之间具有函数相互作用。对味蕾的详细结构的更深入的理解将有助于研究人员对不同的细胞元素之间功能相互作用以及对味觉信息如何从味蕾传播到大脑之间的功能相互作用的假设。 相关性：味蕾是复杂的感觉内虫，对于检测营养和毒素至关重要。尽管在过去的十年中已经阐明了许多分子机制，但我们仍然不了解味蕾的潜水细胞之间的功能组织和相互关系。该提案中的调查将依靠新技术，即串行区块EM来生成详细的味蕾的3维图像，以解决有关神经连通性和细胞电池相互作用在味觉信息从味蕾传播到大脑中的问题。