Scanning Blockface EM of Murine and Human Taste Buds

扫描小鼠和人类味蕾的块面电镜

• 批准号: 9088450
• 负责人: Thomas E Finger
• 金额: $ 32.98万
• 依托单位: UNIVERSITY OF COLORADO DENVER
• 依托单位国家: 美国
• 财政年份: 2015
• 资助国家: 美国
• 起止时间: 2015-07-01 至 2020-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9088450
• 关键词: 3-Dimensional; Anatomy; Architecture; Brain; Cell Communication; Cells; Code; Complex; Data; Databases; Detection; Elements; Exhibits; Face; Funding; Goals; Human; Image; Imagery; Individual; Investigation; Knowledge; Label; Lead; Mitochondria; Molecular; Morphology; Mus; Nerve; Nerve Fibers; Neurotransmitters; Nutrient; Nutritional; Pattern; Peripheral; Peripheral Nervous System; Physiological; Property; Receptor Cell; Receptor Signaling; Reporting; Research Design; Research Personnel; Retinal; Rodent; Scanning; Sensory; Signal Transduction; Site; Source; Specificity; Structure; Synapses; System; Taste Buds; Taste Perception; Technology; Testing; Time; Toxin; Type I Epithelial Receptor Cell; Type II Epithelial Receptor Cell; Visual; afferent nerve; cell type; connectome; detector; information processing; light microscopy; new technology; novel; receptor; reconstruction; relating to nervous system; response; taste system; taste transduction; theories; transmission process; vesicular release

 DESCRIPTION (provided by applicant): Taste buds are complex multicellular endorgans essential for detection of taste. The cells of a taste bud distinguish between the nutritious and the potentially toxic, and communicate this information to the taste nerves innervating them. Despite recent advances in understanding the molecular signaling underlying taste transduction, a complete understanding of cell-cell interactions and transmission of information to the peripheral nervous system is lacking. We propose to use a newly invented technology, serial blockface scanning EM, to generate a complete 3D picture of taste bud structure from both mice and humans in order to test whether taste information is transmitted along dedicated "labeled lines" dedicated to an individual taste quality, or whether the brain receives a more complex signal from the taste buds. The novel EM technology will permit detailed 3D reconstruction of the taste bud connectome allowing for analysis of key features including: 1) whether individual nerve fibers make functional contact with more than one cell type in a bud, and 2) whether the glial-like Type I cells fully separate the other cell types or whether there is potential for functonal interactions between receptor cells encoding the different taste qualities. This greater understanding of the detailed structure of taste buds will help investigators formulate data-based hypotheses regarding functional interactions between the different cellular elements and a more clear understanding of how taste information is transmitted from taste buds to brain. RELEVANCE: Taste buds are complex sensory endorgans crucial for detection of both nutrients and toxins. While many of the molecular mechanisms underlying taste have been elucidated during the last decade, we still do not understand the functional organization and interrelationships between the diverse cells of a taste bud. Investigations in this proposal will rely on new technology, serial blockface EM, to generate detailed 3- dimensional images of taste buds to resolve questions about neural connectivity and cell-cell interactions in transmission of taste information from taste bud to brain.

 描述（由申请人提供）：味蕾是复杂的多细胞终末器官，对味觉检测至关重要。味蕾细胞区分营养和潜在的毒性，并将此信息传达给支配它们的味觉神经。尽管最近在理解味觉传导的分子信号传导方面取得了进展，但缺乏对细胞-细胞相互作用和向外周神经系统传递信息的完整理解。我们建议使用一种新发明的技术，串行块面扫描EM，以生成一个完整的3D图片的味蕾结构从小鼠和人类，以测试是否味道信息是沿着沿着专用的“标记线”专门用于个人的味道质量，或者是否大脑接收到一个更复杂的信号从味蕾。新的EM技术将允许味蕾连接体的详细3D重建，允许分析关键特征，包括：1）单个神经纤维是否与芽中的一种以上细胞类型进行功能性接触，以及2）胶质样I型细胞是否完全分离其他细胞类型，或者编码不同味觉品质的受体细胞之间是否存在功能性相互作用的潜力。对味蕾详细结构的更深入了解将有助于研究人员制定关于不同细胞元素之间功能相互作用的基于数据的假设，并更清楚地了解味觉信息如何从味蕾传递到大脑。 相关性：味蕾是复杂的感觉器官，对检测营养物质和毒素至关重要。虽然在过去的十年中已经阐明了许多味觉的分子机制，但我们仍然不了解味蕾不同细胞之间的功能组织和相互关系。该提案的研究将依赖于新技术，串行块面EM，以生成味蕾的详细三维图像，以解决有关味蕾到大脑的味觉信息传输中的神经连接和细胞间相互作用的问题。