Scanning Blockface EM of Murine and Human Taste Buds

扫描小鼠和人类味蕾的块面电镜

• 批准号: 9813048
• 负责人: Thomas E Finger
• 金额: $ 3.95万
• 依托单位: UNIVERSITY OF COLORADO DENVER
• 依托单位国家: 美国
• 财政年份: 2015
• 资助国家: 美国
• 起止时间: 2015-07-01 至 2020-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9813048
• 关键词: 3-Dimensional; Anatomy; Architecture; Brain; Cell Communication; Cells; Chemicals; Code; Complex; Data; Detection; Elements; Exhibits; Face; Funding; Goals; Human; Image; Imagery; Individual; Investigation; Knowledge; Label; Mitochondria; Molecular; Morphology; Mus; Nerve; Nerve Fibers; Neurotransmitters; Nutrient; Nutritional; Pattern; Peripheral; Peripheral Nervous System; Physiological; Property; Receptor Cell; Receptor Signaling; Reporting; Research Personnel; Retinal; Rodent; Scanning; Sensory; Signal Transduction; Site; Source; Specificity; Structure; Synapses; System; Taste Buds; Taste Perception; Technology; Testing; Time; Toxin; Type I Epithelial Receptor Cell; Type II Epithelial Receptor Cell; Visual; afferent nerve; base; comparative; connectome; design; detector; information processing; light microscopy; neurotransmitter release; new technology; novel; receptor; reconstruction; relating to nervous system; response; taste system; taste transduction; theories; transmission process; vesicular release

PROJECT SUMMARY Taste buds are complex multicellular endorgans essential for detection of taste. The cells of a taste bud distinguish between the nutritious and the potentially toxic, and communicate this information to the taste nerves innervating them. Despite recent advances in understanding the molecular signaling underlying taste transduction, a complete understanding of cell-cell interactions and transmission of information to the peripheral nervous system is lacking. We propose to use a newly invented technology, serial blockface scanning EM, to generate a complete 3D picture of taste bud structure from both mice and humans in order to test whether taste information is transmitted along dedicated “labeled lines” dedicated to an individual taste quality, or whether the brain receives a more complex signal from the taste buds. The novel EM technology will permit detailed 3D reconstruction of the taste bud connectome allowing for analysis of key features including: 1) whether individual nerve fibers make functional contact with more than one cell type in a bud, and 2) whether the glial-like Type I cells fully separate the other cell types or whether there is potential for functional interactions between receptor cells encoding the different taste qualities. This greater understanding of the detailed structure of taste buds will help investigators formulate data-based hypotheses regarding functional interactions between the different cellular elements and a more clear understanding of how taste information is transmitted from taste buds to brain.

项目概要 味蕾是复杂的多细胞内器官，对于味觉检测至关重要。味蕾的细胞 区分有营养的和潜在有毒的，并将此信息传达给口味 支配他们的神经。尽管最近在理解味道背后的分子信号方面取得了进展 转导，对细胞间相互作用和信息传递的完整理解 周围神经系统缺乏。我们建议使用一项新发明的技术，串行blockface 扫描 EM，生成小鼠和人类味蕾结构的完整 3D 图片，以便 测试口味信息是否沿着专用于个人口味的专用“标记线”传输 质量，或者大脑是否从味蕾接收到更复杂的信号。新颖的 EM 技术将 允许对味蕾连接组进行详细的 3D 重建，从而分析关键特征，包括： 1) 单个神经纤维是否与芽中的一种以上细胞类型进行功能性接触，以及 2) 神经胶质样 I 型细胞是否完全分离其他细胞类型，或者是否有功能性潜力 编码不同味道品质的受体细胞之间的相互作用。这样更深入地了解了 味蕾的详细结构将帮助研究人员制定有关功能的基于数据的假设 不同细胞元素之间的相互作用以及对味觉信息如何产生的更清晰的理解 从味蕾传递到大脑。