Cellular Visualization and Analysis

细胞可视化和分析

• 批准号: 7985662
• 负责人: Thomas E Finger
• 金额: $ 19.37万
• 依托单位: UNIVERSITY OF COLORADO DENVER
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-09-01 至 2015-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7985662
• 关键词: 3-Dimensional; Area; Complex; Confocal Microscopy; Data; Data Set; Development; Discipline; Disease; Electron Microscope; Electron Microscopy; Electrophysiology (science); Equipment; Fingers; Functional Imaging; Grant; Health; Histology; Image; Imagery; Imaging technology; In Situ Hybridization; Individual; Laboratories; Lasers; Measures; Methodology; Methods; Microscope; Microscopy; Minor; Modification; Molecular; Neuroanatomy; Participant; Pattern; Photons; Publications; Qualifying; Quality Control; Reagent; Research; Research Personnel; Resources; Sampling; Scanning; Services; System; Techniques; Training; Training Support; Transgenic Animals; base; digital imaging; experience; genetic manipulation; immunocytochemistry; innovation; research study; software development; transmission process

This competing renewal for the RMTSC Core on Cellular Visualization and Analysis seeks to continue support services for histology, neuroanatomy and advanced microscopy for RMTSC investigators. This Core offers 3 types of services: 1) training and expertise in specialized histology (e.g. immunocytochemistry) and neuroanatomy, 2) Specialized microscopy facilities and training, including confocal microscopy and digital imaging, and 3) Quantitative analysis and representation of 3D anatomical data. This Core has been highly successful in the past grant period, being involved in experiments from all RMTSC participating laboratories and assisting in most publications arising from the Center. We propose to continue these functions into the next grant period with only minor modifications to the approach and services offered. The principal functions of this Core include provision of training and assistance in the development and application of contemporary histological methods with an emphasis on immunocytochemistry and in situ hybridization. The Core offers quality control and advice in proper utilization of immunological reagents. In addition, the Core provides anatomical expertise to enable investigators from other fields to properly analyze and interpret anatomical images. In terms of microscopy equipment, the Core maintains a RMTSC laser scanning confocal microscope and supports use of other advanced microscopes including spinning disk confocal, 2-photon confocal, Ca-lmaging workstations and transmission electron microscopes. Finally, the Cellular Visualization and Analysis Core offers expertise and development of software capable of rendering 3-D anatomical data in a quantitative enabled format to permit objective measures of changes in activity patterns, endorgan distribution and cellular activity.

RMTSC核心在蜂窝可视化和分析方面的这种竞争续订旨在继续 RMTSC研究人员的组织学，神经解剖学和晚期显微镜的支持服务。这个核心 提供3种服务类型：1）专业组织学的培训和专业知识（例如免疫细胞化学）和 神经解剖学，2）专门的显微镜设施和培训，包括共聚焦显微镜和数字 成像和3）3D解剖数据的定量分析和表示。这个核心很高 在过去的赠款期间成功，参与了所有RMTSC参与实验室的实验 并协助大多数由中心引起的出版物。我们建议将这些功能继续 下一个赠款期只对提供的方法和服务进行了少量修改。主要功能 该核心包括提供培训和协助在当代的开发和应用中 组织学方法强调免疫细胞化学和原位杂交。核心提供 适当利用免疫学试剂的质量控制和建议。此外，核心提供 解剖专业知识使来自其他领域的研究人员能够正确分析和解释解剖学 图像。在显微镜设备方面，核心保持RMTSC激光扫描共焦 显微镜并支持使用其他高级显微镜，包括旋转磁盘共焦，2-Photon 共聚焦，CA-LMAGG工作站和透射电子显微镜。最后，细胞可视化 分析核心提供了能够在3-D解剖数据中渲染3-D解剖数据的软件的专业知识和开发 启用定量的格式，可以客观地衡量活动模式的变化，Endorgan 分布和细胞活性。