Cellular Visualization and Analysis

这种竞争性的更新RMTSC核心细胞可视化和分析寻求继续 为RMTSC研究者提供组织学、神经解剖学和先进显微镜的支持服务。这一核心 提供3种类型的服务：1）专业组织学（如免疫细胞化学）的培训和专业知识， 神经解剖学，2）专门的显微镜设施和培训，包括共聚焦显微镜和数字显微镜， 成像，以及3）3D解剖数据的定量分析和表示。这一核心一直高度 在过去的资助期内取得了成功，参与了所有RMTSC参与实验室的实验 并协助中心的大多数出版物。我们建议把这些职能延续至 在下一个资助期内，只对所提供的方法和服务作了微小的修改。主要职能 包括提供培训和援助，以发展和应用当代 组织学方法，重点是免疫细胞化学和原位杂交。核心报价 质量控制和正确使用免疫试剂的建议。此外，核心提供 解剖专业知识，使其他领域的研究人员能够正确分析和解释解剖 图像.在显微镜设备方面，核心保持了RMTSC激光扫描共聚焦 显微镜，并支持使用其他先进的显微镜，包括旋转盘共聚焦，2-光子 共焦、钙成像工作站和透射电子显微镜。最后，细胞可视化 和分析核心提供专业知识和软件开发，能够渲染三维解剖数据， 一种量化的使能格式，以便客观衡量活动模式的变化， 分布和细胞活性。

This competing renewal for the RMTSC Core on Cellular Visualization and Analysis seeks to continue support services for histology, neuroanatomy and advanced microscopy for RMTSC investigators. This Core offers 3 types of services: 1) training and expertise in specialized histology (e.g. immunocytochemistry) and neuroanatomy, 2) Specialized microscopy facilities and training, including confocal microscopy and digital imaging, and 3) Quantitative analysis and representation of 3D anatomical data. This Core has been highly successful in the past grant period, being involved in experiments from all RMTSC participating laboratories and assisting in most publications arising from the Center. We propose to continue these functions into the next grant period with only minor modifications to the approach and services offered. The principal functions of this Core include provision of training and assistance in the development and application of contemporary histological methods with an emphasis on immunocytochemistry and in situ hybridization. The Core offers quality control and advice in proper utilization of immunological reagents. In addition, the Core provides anatomical expertise to enable investigators from other fields to properly analyze and interpret anatomical images. In terms of microscopy equipment, the Core maintains a RMTSC laser scanning confocal microscope and supports use of other advanced microscopes including spinning disk confocal, 2-photon confocal, Ca-lmaging workstations and transmission electron microscopes. Finally, the Cellular Visualization and Analysis Core offers expertise and development of software capable of rendering 3-D anatomical data in a quantitative enabled format to permit objective measures of changes in activity patterns, endorgan distribution and cellular activity.