Cellular Visualization and Analysis

细胞可视化和分析

• 批准号: 7985662
• 负责人: Thomas E Finger
• 金额: $ 19.37万
• 依托单位: UNIVERSITY OF COLORADO DENVER
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-09-01 至 2015-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7985662
• 关键词: 3-Dimensional; Area; Complex; Confocal Microscopy; Data; Data Set; Development; Discipline; Disease; Electron Microscope; Electron Microscopy; Electrophysiology (science); Equipment; Fingers; Functional Imaging; Grant; Health; Histology; Image; Imagery; Imaging technology; In Situ Hybridization; Individual; Laboratories; Lasers; Measures; Methodology; Methods; Microscope; Microscopy; Minor; Modification; Molecular; Neuroanatomy; Participant; Pattern; Photons; Publications; Qualifying; Quality Control; Reagent; Research; Research Personnel; Resources; Sampling; Scanning; Services; System; Techniques; Training; Training Support; Transgenic Animals; base; digital imaging; experience; genetic manipulation; immunocytochemistry; innovation; research study; software development; transmission process

This competing renewal for the RMTSC Core on Cellular Visualization and Analysis seeks to continue support services for histology, neuroanatomy and advanced microscopy for RMTSC investigators. This Core offers 3 types of services: 1) training and expertise in specialized histology (e.g. immunocytochemistry) and neuroanatomy, 2) Specialized microscopy facilities and training, including confocal microscopy and digital imaging, and 3) Quantitative analysis and representation of 3D anatomical data. This Core has been highly successful in the past grant period, being involved in experiments from all RMTSC participating laboratories and assisting in most publications arising from the Center. We propose to continue these functions into the next grant period with only minor modifications to the approach and services offered. The principal functions of this Core include provision of training and assistance in the development and application of contemporary histological methods with an emphasis on immunocytochemistry and in situ hybridization. The Core offers quality control and advice in proper utilization of immunological reagents. In addition, the Core provides anatomical expertise to enable investigators from other fields to properly analyze and interpret anatomical images. In terms of microscopy equipment, the Core maintains a RMTSC laser scanning confocal microscope and supports use of other advanced microscopes including spinning disk confocal, 2-photon confocal, Ca-lmaging workstations and transmission electron microscopes. Finally, the Cellular Visualization and Analysis Core offers expertise and development of software capable of rendering 3-D anatomical data in a quantitative enabled format to permit objective measures of changes in activity patterns, endorgan distribution and cellular activity.

RMTSC 细胞可视化和分析核心的竞争更新旨在继续 为 RMTSC 研究人员提供组织学、神经解剖学和高级显微镜学支持服务。这个核心 提供 3 种类型的服务：1) 专门组织学（例如免疫细胞化学）方面的培训和专业知识以及 神经解剖学，2) 专业显微镜设施和培训，包括共焦显微镜和数字显微镜 成像，3) 3D 解剖数据的定量分析和表示。该核心得到了高度评​​价 在过去的资助期内取得了成功，参与了所有 RMTSC 参与实验室的实验 并协助该中心的大多数出版物。我们建议将这些职能继续延续到 下一个资助期，只需对所提供的方法和服务进行较小的修改。主要职能 该核心内容包括在当代技术的开发和应用方面提供培训和援助 组织学方法，重点是免疫细胞化学和原位杂交。核心提供 质量控制和正确使用免疫试剂的建议。此外，该核心还提供 解剖学专业知识，使其他领域的研究人员能够正确分析和解释解剖学 图像。在显微设备方面，Core保留了RMTSC激光扫描共焦 显微镜并支持使用其他先进显微镜，包括转盘共焦、2 光子显微镜 共焦、钙成像工作站和透射电子显微镜。最后，细胞可视化 Analysis Core 提供专业知识和软件开发，能够在 3D 中呈现 3D 解剖数据 一种定量的格式，允许对活动模式的变化进行客观测量，endorgan 分布和细胞活性。