Crystallography pp.623-710

晶体学第 623-710 页

• 批准号: 9233144
• 负责人: KEITH O HODGSON
• 金额: $ 67.85万
• 依托单位: STANFORD UNIVERSITY
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/9233144
• 关键词: Automobile Driving; Biomedical Research; Biophysics; Chemicals; Communities; Complex; Computer software; Crystallography; Data Analyses; Data Collection; Development; Funding; Future; Generations; Methodology; Methods; Molecular Biology; Molecular Machines; National Institute of General Medical Sciences; Performance; Research; Resolution; Resources; Roentgen Rays; Scientific Advances and Accomplishments; Scientist; Services; Source; Spectrum Analysis; Structure; Synchrotrons; System; Techniques; Time; Training Support; United States National Institutes of Health; absorption; biological systems; detector; emission spectroscopy; improved; innovation; instrument; instrumentation; new technology; operation; programs; protein folding; structural biology; synchrotron radiation

Synchrotron radiation (SR) is an extremely bright and tunable x-ray source that enables forefront research in structural molecular biology (SMB). A “Synchrotron Structural Biology Resource" is proposed for continuing support at the Stanford Synchrotron Radiation Lightsource (SSRL) by the NIH NIGMS and DOE BER to develop new technologies in macromolecular crystallography, x-ray absorption/emission spectroscopy and small angle x-ray scattering/diffraction, to train/support users, and to disseminate the newly developed capabilities to the biomedical research community. This proposal is for the continued funding, operation and future development of this SMB Resource. New initiatives will capitalize on the increasing SR performance of SSRL’s 3rd generation storage ring SPEAR3. Proposed also is the development of selected SMB applications of LCLS. A principal aim is to optimize experimental facilities and instrumentation, detectors, software and compute performance on the SMB Resource’s 9+ beam lines at SSRL (with another two in construction) to take full advantage of the high brightness provided by SPEAR3 at 500 mA current and provide innovative new instrumentation and methodologies. This will enable the SMB Resource to advance the scientific forefront with new initiatives built upon state-of-the-art instrumentation and methodologies, innovative software and automated/high-throughput systems for: studying high resolution structures/function of large, complex biomolecules and molecular machines; investigating fundamental questions in biophysics such as protein folding; and developing/improving methods for studying very fast time-resolved structural changes in chemical and biological systems with ultrafast or fast scattering and spectroscopy techniques. These scientific advancements will be facilitated by parallel developments in software to provide expanded capabilities for instrument and detector control, remote data collection and real-time data analysis. Driving biomedical projects and collaborative research and service programs involving a large number of outside scientists will drive and support core technological developments.

同步辐射(SR)是一种非常明亮和可调的X射线源，能够 结构分子生物学(SMB)前沿研究。《同步加速器结构生物学》 提议为斯坦福同步辐射光源提供持续支持 (SSRL)由NIH NIGMS和DOE BER共同开发高分子新技术 结晶学、x射线吸收/发射光谱和小角x射线 散射/绕射，培训/支持用户，传播新开发的能力 给生物医学研究界。这项建议是为了继续提供资金、运营 以及这一中小企业资源的未来发展。新的计划将利用不断增加的 SSRL第三代存储环SPEAR3的SR性能。提出的也是 开发精选的LCLS中小企业应用程序。一个主要目标是优化实验 中小型企业的设施和仪器、探测器、软件和计算性能 资源在SSRL的9条以上的光束线(另外两条正在建设中)，以充分利用 SPEAR3在500 mA电流下提供高亮度，并提供创新的新 仪器和方法。这将使SMB资源能够推进 科学前沿，新举措建立在最先进的仪器和 方法、创新软件和自动化/高通量系统：研究高 大而复杂的生物分子和分子机器的拆分结构/功能； 研究生物物理学的基本问题，如蛋白质折叠；以及 开发/改进研究快速时间分辨结构变化的方法 具有超快或快速散射和光谱技术的化学和生物系统。 这些科学进步将通过软件的并行开发来促进，以提供 扩展了仪器和探测器控制、远程数据采集和实时功能 数据分析。推动生物医学项目以及协作研究和服务计划 大量外部科学家的参与将推动和支持核心技术 事态的发展。