Bloomington Drosophila Stock Center at Indiana University

描述（由申请人提供）：布卢明顿果蝇库存中心（BDSC）支持一个大型的，世界范围内的科学家社区使用昆虫果蝇作为生物医学实验的模式生物。BDSC的目标是提供一系列对当前研究具有广泛价值的记录活种群，保存具有明确未来价值的记录菌株，并提供信息和支持服务，促进最大限度地利用这些材料。这些目标通过提供普遍和快速获得最普遍有用的种群，通过保存具有特殊特征的特殊基因型，以及通过提供帮助研究人员确定适合其需要的种群的信息，促进研究。果蝇被广泛用于生物学研究 果蝇研究人员所掌握的遗传技术是所有多细胞生物中最先进的技术之一，因此，果蝇研究人员可以利用这些技术研究与人类健康相关的过程，并研究疾病的分子机制。作为果蝇遗传实验最全面的库存来源，BDSC对于许多研究项目（包括大量NIH赠款）的成功至关重要。该提案的第一个具体目标是继续获取、维护和分发果蝇品系，并继续开发相关的信息资源，以满足果蝇科学家的研究需求，同时保持和促进优秀的用户支持。实现这一目标的关键是管理和推进非常成功的成本回收计划，该计划从用户费中为业务费用提供资金。因此，该提案侧重于支持和发展核心管理团队，作为利用NIH资源投资的最有效方式。第二个具体目标是进行研究，以增加BDSC股票的一个子集，已被保存其独特的突变表型的效用。这项工作将实验性地将这些股票中的突变映射到基因组序列中的特定转录单位，并将大大增加股票的有用性和相关性，以研究分子定义基因的功能意义。

DESCRIPTION (provided by applicant): The Bloomington Drosophila Stock Center (BDSC) supports a large, worldwide community of scientists using the insect Drosophila melanogaster as a model organism for biomedical experimentation. The goals of the BDSC are to provide a collection of documented living stocks of broad value to current research, to preserve documented strains with clear future value, and to provide information and support services that promote maximal exploitation of these materials. These goals facilitate research by providing universal and rapid access to the most generally useful stocks, by preserving specialty genotypes with exceptional characteristics, and by providing information that helps researchers identify stocks appropriate to their needs. Drosophila is used extensively in studies of biological processes relevant to human health and investigations of molecular mechanisms underlying disease, because genetic technologies available to Drosophila researchers are among the most sophisticated in any multicellular organism. As the most comprehensive source of stocks for genetic experimentation with Drosophila, the BDSC is central to the success of many research projects including a large number of NIH grants. The first specific aim of this proposal is to continue acquiring, maintaining and distributing Drosophila strains and to continue developing associated information resources to meet the research needs of Drosophila scientists while maintaining and promoting excellent user support. Key to this aim is the administration and advancement of the highly successful cost recovery program that finances operational expenses from user fees. Consequently, the proposal focuses on support and development of the core management team as the most effective way to leverage the investment of NIH resources. The second specific aim is to undertake research to increase the utility of a subset of BDSC stocks which have been preserved for their distinctive mutant phenotypes. The work will experimentally map mutations in these stocks to specific transcription units in the genome sequence and will substantially increase the usefulness and relevance of the stocks to researchers investigating the functional significance of molecularly defined genes.