Long Lasting Organic Semiochemical Formulation to Effectively Manage Blood Sucking Diptera

持久的有机化学制剂可有效控制吸血双翅目

• 批准号: 9409577
• 负责人: Agenor Mafra-Neto
• 金额: $ 50万
• 依托单位: ISCA TECHNOLOGIES, INC.
• 依托单位国家: 美国
• 财政年份: 2017
• 资助国家: 美国
• 起止时间: 2017-07-12 至 2019-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9409577
• 关键词: Address; Adopted; Adoption; Aedes; Affect; Africa; African; Aging; Anopheles Genus; Application procedure; Area; Bacillus (bacterium); Bacillus thuringiensis; Beds; Behavior; Biological Assay; Blood; Breeding; Cessation of life; Characteristics; Chemicals; Child; Collaborations; Culex (Genus); Culicidae; Data; Deposition; Developed Countries; Developing Countries; Development; Diptera; Disease; Disease Vectors; Ensure; Environment; Environmental Monitoring; Equipment; Event; Female; Floods; Formulation; Geographic Locations; Government; Gravid; Gravidity; Growth; Health; Human; Industrialization; Ingestion; Insecta; Insecticide Resistance; Insecticides; Intervention; Laboratories; Larva; Life; Life Cycle Stages; Location; Malaria; Manuals; Mechanics; Medical; Methods; Mosquito Control; Mosquito-borne infectious disease; Oviposition; Pattern; Performance; Pest Control; Pesticides; Phase; Pheromone; Population; Practice Management; Prevalence; Privatization; Rain; Reproduction; Resistance development; Resources; Rest; Risk; Science; Seasons; Semiochemicals; Site; Small Business Innovation Research Grant; Source; Submersion; Surface; Technology; Testing; Time; United States; United States National Institutes of Health; Vector-transmitted infectious disease; Water; Work; Yeasts; aged; base; commercialization; controlled release; cost effective; design; disease transmission; efficacy study; egg; feeding; improved; innovation; killings; novel; novel strategies; offspring; pathogen; preference; programs; sucking; tool; toxicant; transmission process; vector; vector control; vector mosquito

7. PROJECT SUMMARY/ABSTRACT We propose to develop SPLAT BAC, a novel mosquito control formulation targeting multiple species of disease vectors around the world, through a controlled-release formulation of bacterial larvicides, Bacillus thuringiensis subsp. israelensis and Bacillus sphaericus. SPLAT BAC is intended for outdoor application prior to the rainy season, a characteristic that represents an invaluable advantage over previous mosquito control methods, most of which target only indoor-feeding mosquitoes, and rely upon conventional insecticides. SPLAT BAC is an environmentally friendly, sprayable formulation that will be applied to flood plains and other mosquito breeding sites up to one month before the start of the rain season, and will affix to the application site until being submerged by a flooding event. The SPLAT BAC point sources will detach from their substrates following a submersion period designed to correspond with peak larval emergence, and float to the surface of the water, where most mosquito larvae rest and feed, thereby ensuring the release of the bacterial larvicides into the larvae's environment at the precise time and location that they will be most likely to respond to and feed upon them. The Phase II objectives designed to further the development of this novel pest control solution are as follows: 1. Create a universal SPLAT BAC mosquito larvicide formulation, effective against multiple mosquito vectors worldwide. Laboratory bioassays and efficacy studies will be conducted against several mosquito vector species, evaluating the performance of SPLAT BAC with additional attractive components: oviposition attractants and two promising phagostimulants, blood meal and yeast, as well as field aging and environmental tests to ensure that SPLAT BAC behaves as required for a preemptive mosquito control product. 2. Optimize SPLAT BAC for ultra-versatile and cost-effective application with manual, mechanical, ground, and aerial equipment. A broad range of manual and mechanical application equipment will be tested with SPLAT BAC in the lab, both for ease of use and to determine the impact of application method upon the product's efficacy and field life. Specialized Backpack and ATV-based equipment will also be designed specifically for SPLAT BAC. 3. Demonstrate the potential of SPLAT BAC for adoption by mosquito control programs in the United States. This work will be conducted in collaboration with vector control professionals in multiple states to evaluate the potential of SPLAT BAC for incorporation into public and private mosquito control programs. 4. Develop SPLAT BAC application procedures for effective, affordable mosquito control in the developing world where MBDs have the most devastating impacts BAC will be tested for its efficacy against key African vector species, as well as its ability to function as designed under the more challenging environmental conditions present in tropical regions. Large-scale field trials will also be conducted with the cooperation of mosquito control professionals in east Africa, to determine SPLAT BAC's capacity to suppress vector populations in this region, as well as the most effective application methods for these environments.

7.项目总结/摘要 我们建议开发SPLAT BAC，一种针对世界各地多种疾病媒介的新型蚊虫控制制剂，通过控制释放的细菌杀幼虫剂苏云金芽孢杆菌亚种。israelensis和球形芽孢杆菌。SPLAT BAC旨在雨季前用于室外应用，这一特性代表了以往蚊子控制方法的宝贵优势，其中大多数仅针对室内喂养的蚊子，并依赖于传统杀虫剂。SPLAT BAC是一种环保的可喷雾制剂，将在雨季开始前一个月应用于洪泛平原和其他蚊子繁殖地，并将附着在应用地点，直到被洪水淹没。SPLAT BAC点源将在设计为与幼虫出现高峰相对应的浸没期之后从其基质分离，并漂浮到水面，大多数蚊子幼虫在那里休息和进食，从而确保在幼虫最有可能对其作出反应并以其为食的精确时间和位置将细菌杀幼虫剂释放到幼虫的环境中。第二阶段的目标是进一步开发这种新型的害虫控制解决方案，具体如下：1.创建一种通用的SPLAT BAC杀蚊幼虫剂配方，可有效杀灭全球多种蚊媒。将对几种蚊子媒介物种进行实验室生物测定和功效研究，评估SPLAT BAC与其他吸引成分的性能：产卵引诱剂和两种有前途的诱食剂，血粉和酵母，以及现场老化和环境测试，以确保SPLAT BAC的行为符合先发制人的蚊子控制产品的要求。2.优化SPLAT BAC，实现手动、机械、地面和空中设备的超多功能和经济高效的应用。将在实验室中使用SPLAT BAC对各种手动和机械施用设备进行测试，以便于使用并确定施用方法对产品功效和田间寿命的影响。专门的背包和基于ATV的设备也将专门为SPLAT BAC设计。3.展示SPLAT BAC在美国蚊子控制项目中的应用潜力。这项工作将与多个州的病媒控制专业人员合作进行，以评估SPLAT BAC纳入公共和私人蚊虫控制计划的潜力。4.制定SPLAT生物活性炭应用程序，以便在发展中国家有效、负担得起地控制蚊子，在这些国家，多溴联苯具有最具破坏性的影响。还将与东非的蚊虫控制专业人员合作进行大规模的实地试验，以确定SPLAT BAC在该地区抑制病媒种群的能力，以及最有效的 适用于这些环境。