Polysaccharide-Polyamine Hydrogels

 描述（由申请人提供）：水凝胶是能够吸收和保留大量水的三维网状聚合物。近年来，水凝胶作为药物释放的刺激响应性材料和组织工程支架材料得到了广泛的应用。在商业上，它们是各种材料（包括接触透镜和伤口敷料）中可行的和不可或缺的组分。Gerecht及其同事最近的一项研究表明，胺官能化的葡聚糖水凝胶可以在不使用生长因子或维生素等添加剂的情况下加速血管生成和新血管形成。我们最近开发了一种简易的通用1-步法将多糖和多胺掺入水凝胶中。使用这种方法，我们提出了一个研究多糖和胺如何影响伤口愈合。通过体外方法，使用细胞迁移和增殖试验评价水凝胶。将沿着不同的多胺检查具有不同组成的碳水化合物的多糖，并将其与Gerecht聚合物进行比较。还制备了基于姜黄素的多胺，并将检查其对伤口愈合的作用。姜黄素是一种抗氧化、抗炎的多酚，并且在体外和体内伤口愈合研究中都显示出作为伤口愈合治疗剂的成功。姜黄素已显示诱导应激反应并导致伤口愈合的密封诱导。我们已经制备了一种可生物降解的姜黄素多胺聚合物，并使用我们开发的方法将其整合到水凝胶中，并将检查这些材料的伤口愈合能力。姜黄素还具有抗多种癌症的活性，这种水凝胶将 也可以作为癌症治疗剂进行研究。关键词：水凝胶，抗菌，伤口愈合，癌症

 DESCRIPTION (provided by applicant): Hydrogels are three-dimensional networked polymers capable of absorbing and retaining copious quantities of water. Recently, hydrogels have been prominently featured as stimuli responsive materials for drug delivery and as scaffolds in tissue engineering. Commercially, they are viable and integral components in various materials, including contact lens and wound dressings. A recent study by Gerecht and coworkers has shown an amine functionalized dextran hydrogel to speed angiogenesis and neovascularization without the use of additives like grow factors or vitamins. We have recently developed a facile universal 1-step approach to incorporate polysaccharides and polyamines into hydrogels. Using this methodology, we propose a study of how the polysaccharide and amine affects wound healing. The hydrogels are to be evaluated by in vitro methods, using cell migration and proliferation assays. Polysaccharides with varying comprising carbohydrates will be examined along with varying polyamines and will be compared against the Gerecht polymer. A curcumin based polyamine has also been prepared and its effect on wound healing will be examined. Curcumin is an anti-oxidant, anti-inflammatory polyphenol and has shown success as a wound healing therapeutic in both in vitro and in vivo wound healing studies. Curcumin has been shown to induce a stress response and results in the hermetic induction of wound healing. We have prepared a biodegradable curcumin polyamine polymer and have integrated it into a hydrogel using our developed methodology and will examine these materials for their wound healing ability. Curcumin also has activity against a broad array of cancers and this hydrogel will also be examined as a cancer therapeutic. Keywords: Hydrogels, antimicrobial, wound healing, cancer