Polysaccharide-Polyamine Hydrogels

 描述(申请人提供)：水凝胶是一种三维网络聚合物，能够吸收和保持大量的水。近年来，水凝胶作为药物释放的刺激响应性材料和组织工程中的支架材料已成为人们关注的焦点。在商业上，它们是各种材料的可行和不可或缺的组成部分，包括隐形眼镜和伤口敷料。Gerecht和他的同事最近的一项研究表明，一种胺功能化的葡聚糖水凝胶可以在不使用生长因子或维生素等添加剂的情况下加速血管生成和新生血管。我们最近开发了一种简便、通用的一步法，将多糖和多胺结合到水凝胶中。利用这种方法，我们提出了一项关于多糖和胺如何影响伤口愈合的研究。水凝胶将通过体外方法进行评估，使用细胞迁移和增殖分析。含有不同碳水化合物的多糖将与不同的多胺一起进行检测，并将与Gerecht聚合物进行比较。还制备了一种基于姜黄素的多胺，并将检测其对伤口愈合的影响。姜黄素是一种抗氧化剂、抗炎多酚，在体外和体内伤口愈合研究中都被证明是一种成功的伤口愈合疗法。姜黄素已被证明可以诱导应激反应，并导致密闭诱导伤口愈合。我们已经制备了一种可生物降解的姜黄素多胺聚合物，并使用我们开发的方法将其整合到水凝胶中，并将检测这些材料的伤口愈合能力。姜黄素还具有抗多种癌症的活性，这种水凝胶将 也可以作为癌症治疗药物进行检查。关键词：水凝胶、抗菌剂、伤口愈合、癌症

 DESCRIPTION (provided by applicant): Hydrogels are three-dimensional networked polymers capable of absorbing and retaining copious quantities of water. Recently, hydrogels have been prominently featured as stimuli responsive materials for drug delivery and as scaffolds in tissue engineering. Commercially, they are viable and integral components in various materials, including contact lens and wound dressings. A recent study by Gerecht and coworkers has shown an amine functionalized dextran hydrogel to speed angiogenesis and neovascularization without the use of additives like grow factors or vitamins. We have recently developed a facile universal 1-step approach to incorporate polysaccharides and polyamines into hydrogels. Using this methodology, we propose a study of how the polysaccharide and amine affects wound healing. The hydrogels are to be evaluated by in vitro methods, using cell migration and proliferation assays. Polysaccharides with varying comprising carbohydrates will be examined along with varying polyamines and will be compared against the Gerecht polymer. A curcumin based polyamine has also been prepared and its effect on wound healing will be examined. Curcumin is an anti-oxidant, anti-inflammatory polyphenol and has shown success as a wound healing therapeutic in both in vitro and in vivo wound healing studies. Curcumin has been shown to induce a stress response and results in the hermetic induction of wound healing. We have prepared a biodegradable curcumin polyamine polymer and have integrated it into a hydrogel using our developed methodology and will examine these materials for their wound healing ability. Curcumin also has activity against a broad array of cancers and this hydrogel will also be examined as a cancer therapeutic. Keywords: Hydrogels, antimicrobial, wound healing, cancer