TR&D1-HTS

项目概要/摘要 TR&D1 本项目的目标是开发基于超导的高灵敏度探头 共振器用于代谢组学和蛋白质结构NMR的直接检测。为 代谢组学，我们将开发一种对2 H和13 C通道都敏感的探针。这 探针将用于同位素异构体分析以评估代谢变化。蛋白质结构 NMR，探针将被优化以有效检测15 N。该探测器将被设计为 利用最近描述的基于TROSY的线窄化效应，在高场下15 N， 这对于本质无序和膜- 相关蛋白质我们还将利用这些探测器作为技术开发项目， 提高核磁共振探头中超导谐振器的性能。

Project Summary/Abstract TR&D1 The goal of this project is to develop high sensitivity probes based on superconducting resonators for direct detection in metabolomics and in protein structure NMR. For metabolomics, we will develop a probe which is sensitive on both 2H and 13C channels. This probe will be used for isotopomer analysis to assess metabolic changes. For protein structure NMR, the probe will be optimized for efficient detection of 15N. This probe will be designed to exploit the recently described TROSY-based line narrowing effect for 15N at high field, an effect which will be important for structure characterization of intrinsically disordered and membrane- associated proteins. We will also use these probes as technology development projects to improve the performance of superconducting resonators in NMR probes.