Solution-Phase Biomolecular NMR at 24 T Fields Using New High Temperature Superco

使用新型高温 Superco 在 24 T 场进行溶液相生物分子 NMR

• 批准号: 9058563
• 负责人: William W Brey
• 金额: $ 17万
• 依托单位: FLORIDA STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2014
• 资助国家: 美国
• 起止时间: 2014-08-01 至 2018-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9058563
• 关键词: Awareness; Biomedical Research; Biomolecular Nuclear Magnetic Resonance; Cells; Communities; Complex; Crystallization; Data; Dependence; Development; Drug Industry; Environmental Wind; Face; Feedback; Filament; Future; Goals; Health; Heating; High temperature of physical object; Joints; Lead; Length; Location; Magnetic Resonance; Magnetic Resonance Imaging; Magnetic Resonance Spectroscopy; Membrane Proteins; NMR Spectroscopy; Nucleic Acids; Operating System; Pharmacologic Substance; Phase; Physiological; Proteins; Radiology Specialty; Recording of previous events; Research; Research Personnel; Resolution; Resources; Rest; Role; Running; Sampling; Science; Signal Transduction; Societies; Spottings; Structure; System; Techniques; Technology; Temperature; Time; United States National Institutes of Health; base; clinical Diagnosis; cold temperature; density; design; drug development; healthcare community; improved; insight; instrument; magnetic field; next generation; prevent; prototype; research study; screening; tool; wound

DESCRIPTION (provided by applicant): NMR is central to both the academic and industrial biomedical communities. Further progress in NMR is challenged by ultimate limitations in the low-temperature superconductor (LTS) wires used in NMR/MRI magnets since the 1970s, that prevent them from operating beyond 24T in a persistent, non-driven mode. This proposal builds on a recent NHMFL-based development that has delivered the first coil operating in a stable superconducting state at fields e34 T, and proposes to use this breakthrough for building a fully- superconducting solution NMR system operating above 1 GHz. To achieve this, and to demonstrate the feasibility of exploiting these ideas to carry biomolecular solution-state NMR to this and at higher (H30T) magnetic fields, we aim to: (i) develop a high homogeneity coil of round multifilamentary Bi-2212 wire, a new kind of high-temperature superconductor (HTS) developed by Larbalestier et al in 2012 and that unlike previously proposed HTSs can carry a high current density beyond 30 T; (ii) operate this coil within an existing Oxford Instrument LTS outsert magnet running in persistent mode, to deliver a room-temperature magnetic field sweet spot in excess of 23.5 T over a 10 mm DSV with homogeneities and relevant field instabilities of H1- 2 ppm; (iii) exploit the expertise that over the last decade NHMFL has amassed in field stabilization and probe construction, to demonstrate the feasibility of using such a setup at room temperature with H30 ppb stabilities and homogeneities over 150 �L samples; (iv) place this system to the disposition of the NMR community at large as part of NHMFL's facility role, in order to exploit it for paramagnetic, membrane-protein, in cell and protein bioNMR research, as well as to get critical feedback on ways to further pursue this breakthrough.

描述(由申请人提供)：核磁共振是学术和工业生物医学界的核心。核磁共振的进一步进展受到了自20世纪70年代以来用于核磁共振/磁共振磁体的低温超导(LTS)导线的极限限制的挑战，这些限制阻止了它们以持续的非驱动模式在24T以上运行。这项提议建立在最近一项基于NHMFL的发展的基础上，该发展已经提供了第一个在e34T场以稳定超导状态工作的线圈，并建议利用这一突破来建立一个工作在1 GHz以上的完全超导溶液核磁共振系统。为了实现这一点，并证明利用这些想法将生物分子溶液态核磁共振携带到这个和更高(H30T)磁场中的可行性，我们的目标是：(I)开发一种高均匀度的圆形多丝Bi2212丝线圈，这是Larbalstier等人在2012年开发的一种新型高温超导体(HTS)，与以前提出的HTS不同，HTS可以携带超过30T的大电流密度；(Ii)在以持续模式运行的现有牛津仪器的输出磁体中操作该线圈，以在具有H1-2ppm的均匀性和相关的场不稳定性的10 mm数字电压上提供超过23.5T的室温磁场最佳点；(Iii)利用NHMFL在过去十年中积累的在磁场稳定和探头构造方面积累的专业知识，以证明在室温下使用具有H30 ppb稳定性和均匀性的这种设置的可行性，超过150个�L样品；(4)作为NHMFL设施作用的一部分，将该系统置于整个核磁共振社区的部署中，以便将其用于顺磁、膜-蛋白质、细胞和蛋白质生物核磁共振研究，并就进一步寻求这一突破的方法获得批判性反馈。

项目成果

Experimental Study on Potential Heat Treatment Issues of Large Bi-2212 Coils.

大型 Bi-2212 卷材潜在热处理问题的实验研究。

• DOI: 10.1109/tasc.2017.2652849
• 发表时间: 2017
• 期刊: IEEE transactions on applied superconductivity : a publication of the IEEE Superconductivity Committee
• 作者: Chen,P;Trociewitz,UP;Lu,J;Bosque,E;Jiang,J;Hellstrom,E;Larbalestier,DC
• 通讯作者: Larbalestier,DC

Process to densify Bi2Sr2CaCu2Ox round wire with overpressure before coil winding and final overpressure heat treatment.

在线圈绕制和最终过压热处理之前，通过过压使 Bi2Sr2CaCu2Ox 圆线致密化的过程。

• DOI: 10.1088/1361-6668/ab5ad6
• 发表时间: 2020
• 期刊: Superconductor science & technology
• 影响因子: 3.6
• 作者: Matras,MR;Jiang,J;Trociewitz,UP;Larbalestier,DC;Hellstrom,EE
• 通讯作者: Hellstrom,EE

Bi-2223 Test Coils for High Resolution NMR Magnets.

用于高分辨率 NMR 磁体的 Bi-2223 测试线圈。

• DOI: 10.1109/tasc.2017.2652378
• 发表时间: 2017
• 期刊: IEEE transactions on applied superconductivity : a publication of the IEEE Superconductivity Committee
• 作者: Marshall,WS;Bird,MD;Godeke,A;Larbalestier,DC;Markiewicz,WD;White,JM
• 通讯作者: White,JM

Effect of sheath material and reaction overpressure on Ag extrusions into the TiO2 insulation coating of Bi-2212 round wire.

护套材料和反应超压对 Ag 挤出到 Bi-2212 圆线 TiO2 绝缘涂层中的影响。

• DOI: 10.1088/1757-899x/279/1/012021
• 发表时间: 2017
• 期刊: IOP conference series. Materials science and engineering
• 作者: Hossain,I;Jiang,J;Matras,M;Trociewitz,UP;Lu,J;Kametani,F;Larbalestier,D;Hellstrom,E
• 通讯作者: Hellstrom,E

Fabrication and Testing of a Bi-2223 Test Coil for High Field NMR Magnets.

用于高场 NMR 磁体的 Bi-2223 测试线圈的制造和测试。

• DOI: 10.1109/tasc.2018.2801296
• 发表时间: 2018
• 期刊: IEEE transactions on applied superconductivity : a publication of the IEEE Superconductivity Committee
• 作者: Marshall,WS;Bird,MD;Larbalestier,DC;McRae,DM;Noyes,PD;Voran,AJ;Walsh,RP
• 通讯作者: Walsh,RP