Solution-Phase Biomolecular NMR at 24 T Fields Using New High Temperature Superco

使用新型高温 Superco 在 24 T 场进行溶液相生物分子 NMR

• 批准号: 8752012
• 负责人: William W Brey
• 金额: $ 17万
• 依托单位: FLORIDA STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2014
• 资助国家: 美国
• 起止时间: 2014-08-01 至 2017-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8752012
• 关键词: Awareness; Biomedical Research; Biomolecular Nuclear Magnetic Resonance; Calculi; Cells; Communities; Community Healthcare; Complex; Crystallization; Data; Dependence; Development; Drug Industry; Environmental Wind; Face; Feedback; Filament; Future; Goals; Heating; High temperature of physical object; Joints; Lead; Length; Location; Magnetic Resonance; Magnetic Resonance Imaging; Magnetic Resonance Spectroscopy; Membrane Proteins; Nucleic Acids; Operating System; Pharmacologic Substance; Phase; Physiological; Proteins; Radiology Specialty; Recording of previous events; Research; Research Personnel; Resolution; Resources; Rest; Role; Running; Sampling; Science; Signal Transduction; Societies; Solutions; Spottings; Structure; System; Techniques; Technology; Temperature; Time; United States National Institutes of Health; base; clinical Diagnosis; cold temperature; density; design; drug development; improved; insight; instrument; magnetic field; next generation; prevent; prototype; public health relevance; research study; screening; tool; wound

DESCRIPTION (provided by applicant): NMR is central to both the academic and industrial biomedical communities. Further progress in NMR is challenged by ultimate limitations in the low-temperature superconductor (LTS) wires used in NMR/MRI magnets since the 1970s, that prevent them from operating beyond 24T in a persistent, non-driven mode. This proposal builds on a recent NHMFL-based development that has delivered the first coil operating in a stable superconducting state at fields e34 T, and proposes to use this breakthrough for building a fully- superconducting solution NMR system operating above 1 GHz. To achieve this, and to demonstrate the feasibility of exploiting these ideas to carry biomolecular solution-state NMR to this and at higher (H30T) magnetic fields, we aim to: (i) develop a high homogeneity coil of round multifilamentary Bi-2212 wire, a new kind of high-temperature superconductor (HTS) developed by Larbalestier et al in 2012 and that unlike previously proposed HTSs can carry a high current density beyond 30 T; (ii) operate this coil within an existing Oxford Instrument LTS outsert magnet running in persistent mode, to deliver a room-temperature magnetic field sweet spot in excess of 23.5 T over a 10 mm DSV with homogeneities and relevant field instabilities of H1- 2 ppm; (iii) exploit the expertise that over the last decade NHMFL has amassed in field stabilization and probe construction, to demonstrate the feasibility of using such a setup at room temperature with H30 ppb stabilities and homogeneities over 150 ¿L samples; (iv) place this system to the disposition of the NMR community at large as part of NHMFL's facility role, in order to exploit it for paramagnetic, membrane-protein, in cell and protein bioNMR research, as well as to get critical feedback on ways to further pursue this breakthrough.

描述（由申请人提供）：NMR是学术和工业生物医学界的核心。自20世纪70年代以来，NMR/MRI磁体中使用的低温超导体（LTS）导线的极限限制对NMR的进一步进展提出了挑战，这阻止了它们以持久的非驱动模式在24 T以上运行。该提议建立在最近的基于NHMFL的开发的基础上，该开发已经交付了在场e34 T处以稳定超导状态操作的第一线圈，并且提议使用该突破来构建在1GHz以上操作的全超导溶液NMR系统。为了实现这一点，并证明利用这些想法将生物分子溶液状态NMR带到这个和更高（H30 T）磁场的可行性，我们的目标是：（i）开发高均匀性的圆形多芯Bi-2212线线圈，一种新型高温超导体由Larbalestier等人在2012年开发，并且与先前提出的HTS不同，HTS可以承载超过30 T的高电流密度;（ii）在以持续模式运行的现有牛津仪器LTS外插磁体内操作该线圈，在10 mm DSV上提供超过23.5 T的室温磁场最佳点，均匀性和相关场不稳定性为H1- 2 ppm;（iii）利用过去十年NHMFL在磁场稳定和探针结构方面积累的专业知识，证明在室温下使用这种装置的可行性，H30 ppb的稳定性和150升样品的均匀性;（iv）将该系统作为NHMFL设施作用的一部分，供广大NMR界使用，以便将其用于细胞和蛋白质生物NMR研究中的顺磁性、膜蛋白质，并就如何进一步实现这一突破获得重要反馈。