Solution-Phase Biomolecular NMR at 24 T Fields Using New High Temperature Superco

使用新型高温 Superco 在 24 T 场进行溶液相生物分子 NMR

• 批准号: 8898853
• 负责人: William W Brey
• 金额: $ 29.75万
• 依托单位: FLORIDA STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2014
• 资助国家: 美国
• 起止时间: 2014-08-01 至 2017-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8898853
• 关键词: Awareness; Biomedical Research; Biomolecular Nuclear Magnetic Resonance; Calculi; Cells; Communities; Community Healthcare; Complex; Crystallization; Data; Dependence; Development; Drug Industry; Environmental Wind; Face; Feedback; Filament; Future; Goals; Health; Heating; High temperature of physical object; Joints; Lead; Length; Location; Magnetic Resonance; Magnetic Resonance Imaging; Magnetic Resonance Spectroscopy; Membrane Proteins; Nucleic Acids; Operating System; Pharmacologic Substance; Phase; Physiological; Proteins; Radiology Specialty; Recording of previous events; Research; Research Personnel; Resolution; Resources; Rest; Role; Running; Sampling; Science; Signal Transduction; Societies; Solutions; Spottings; Structure; System; Techniques; Technology; Temperature; Time; United States National Institutes of Health; base; clinical Diagnosis; cold temperature; density; design; drug development; improved; insight; instrument; magnetic field; next generation; prevent; prototype; research study; screening; tool; wound

DESCRIPTION (provided by applicant): NMR is central to both the academic and industrial biomedical communities. Further progress in NMR is challenged by ultimate limitations in the low-temperature superconductor (LTS) wires used in NMR/MRI magnets since the 1970s, that prevent them from operating beyond 24T in a persistent, non-driven mode. This proposal builds on a recent NHMFL-based development that has delivered the first coil operating in a stable superconducting state at fields e34 T, and proposes to use this breakthrough for building a fully- superconducting solution NMR system operating above 1 GHz. To achieve this, and to demonstrate the feasibility of exploiting these ideas to carry biomolecular solution-state NMR to this and at higher (H30T) magnetic fields, we aim to: (i) develop a high homogeneity coil of round multifilamentary Bi-2212 wire, a new kind of high-temperature superconductor (HTS) developed by Larbalestier et al in 2012 and that unlike previously proposed HTSs can carry a high current density beyond 30 T; (ii) operate this coil within an existing Oxford Instrument LTS outsert magnet running in persistent mode, to deliver a room-temperature magnetic field sweet spot in excess of 23.5 T over a 10 mm DSV with homogeneities and relevant field instabilities of H1- 2 ppm; (iii) exploit the expertise that over the last decade NHMFL has amassed in field stabilization and probe construction, to demonstrate the feasibility of using such a setup at room temperature with H30 ppb stabilities and homogeneities over 150 �L samples; (iv) place this system to the disposition of the NMR community at large as part of NHMFL's facility role, in order to exploit it for paramagnetic, membrane-protein, in cell and protein bioNMR research, as well as to get critical feedback on ways to further pursue this breakthrough.

描述（由申请人提供）：NMR 是学术界和工业生物医学界的核心。自 20 世纪 70 年代以来，用于 NMR/MRI 磁体的低温超导 (LTS) 线的极限限制对 NMR 的进一步发展提出了挑战，这使得它们无法在持续、非驱动模式下运行超过 24T。该提案以最近基于 NHMFL 的开发为基础，该开发已在 e34 T 场交付了第一个在稳定超导状态下运行的线圈，并建议利用这一突破来构建运行在 1 GHz 以上的全超导解决方案 NMR 系统。为了实现这一目标，并证明利用这些想法在更高 (H30T) 磁场下进行生物分子溶液态 NMR 的可行性，我们的目标是：(i) 开发圆形多丝 Bi-2212 线的高均匀性线圈，这是 Larbalestier 等人于 2012 年开发的一种新型高温超导体 (HTS)，与之前提出的 HTS 不同，它可以携带 超过30T的高电流密度； (ii) 在以持续模式运行的现有牛津仪器 LTS 外置磁体中操作该线圈，以在 10 mm DSV 上提供超过 23.5 T 的室温磁场最佳点，并具有 H1-2 ppm 的均匀性和相关场不稳定性； (iii) 利用 NHMFL 在过去十年中在现场稳定和探针构建方面积累的专业知识，证明在室温下使用这种装置的可行性，在 150 L 样品上具有 H30 ppb 稳定性和均匀性； (iv) 作为 NHMFL 设施角色的一部分，将此系统置于整个 NMR 界的配置中，以便将其用于顺磁性、膜蛋白、细胞和蛋白质生物 NMR 研究，并获得有关进一步追求这一突破的方法的关键反馈。