National Resource For Advanced NMR Technology

国家先进核磁共振技术资源

• 批准号: 9280017
• 负责人: William W Brey
• 金额: $ 166.39万
• 依托单位: FLORIDA STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2017
• 资助国家: 美国
• 起止时间: 2017-08-01 至 2022-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9280017
• 关键词: 2,4-Dinitrophenol; Address; Alzheimer' s Disease; Applied Research; Automobile Driving; Bacterial Drug Resistance; Basic Science; Biological; Biology; Biomedical Research; Biomedical Technology; Chemistry; Communities; Complement; Complex; Complex Mixtures; Crystallography; Diabetes Mellitus; Disease; Drug Targeting; Educational workshop; Electrons; Funding; Generations; Goals; HIV; High temperature of physical object; Hybrids; Insulin Resistance; International; Kinetics; Lead; Liquid substance; Macromolecular Complexes; Mass Spectrum Analysis; Membrane Proteins; Metabolic; Microbial Biofilms; Modeling; NMR Spectroscopy; Nuclear; Nuclear Magnetic Resonance; Proteins; Protons; Publications; RF coil; Research; Resolution; Resources; Roentgen Rays; Science; Series; Services; Signal Transduction; Structure; Techniques; Technology; Temperature; Time; Training; Training Activity; Tuberculosis; United States National Institutes of Health; Virus Replication; X-Ray Crystallography; design and construction; drug development; frontier; insight; instrument; instrumentation; interest; lectures; macromolecule; meetings; metabolomics; new technology; news; novel; open data; programs; protein complex; scientific organization; solid state nuclear magnetic resonance; structural biology; tool; web site

National Resource for Advanced NMR Technology OVERALL - Project Summary/Abstract Nuclear magnetic resonance (NMR) spectroscopy is a unique set of experimental tools for understanding the intricacies of biology from macromolecular complexes to complex mixtures, from atomic resolution structure to dynamics on timescales of picoseconds to kiloseconds, from chemistry to functional mechanisms and kinetic rates. No other technology has such breadth and potential for basic and applied research and for interfacing with other technologies, such as X-ray crystallography, small angle X-ray scattering, Cryo-EM, and many other spectroscopic tools. Structural characterization serves as the starting point, providing a framework for understanding biological activities, functional mechanisms and kinetic models that can be added by NMR. Dynamics can be exceptionally well characterized by NMR and this can lead to detailed understanding about how proteins and other macromolecules function, how complexes are formed, and sometimes how kinetic rates are achieved. The solution NMR spectroscopy of complex mixtures has been shown to be particularly useful in combination with mass spectrometry for metabolomics and other complex mixtures. Here, we focus on the frontiers of NMR technology made possible by recent breakthroughs in materials research and instrumentation, and their implementation for a broad user community interested in pursuing fundamental questions at atomic resolution at the forefront of biomedical research. Three TR&Ds advance the sensitivity of NMR each with novel technology – the first through use of high temperature superconductors for RF coils leading to unique sensitivity for solution NMR spectroscopy. TR&D2 takes advantage of a 600 MHz DNP instrument recently installed at the Magnet Lab that will provide enhanced sensitivity through the transfer of magnetization from electrons to protons. New and much more robust DNP probes with an expanded temperature range will be developed. TR&D3 takes advantage of the 36T Series Connected Hybrid for NMR spectroscopy – a jump in field strength of more that 50% equivalent to a jump in field strength from 17T (1990) to 23.5T (2016) that occurred over the past 26 years! This will lead to dramatic enhancements in sensitivity and even more spectacular reductions in signal averaging time. The science will be driven by an excellent team of DBPs and even more C&Ss that span a very broad range of science. A major team effort will be placed on training a new generation of NMR users through an annual pair of workshops and dissemination through publications and presentations at meetings, through a wide variety of scientific organizations and the news media; through a dedicated website for this Resource and through our training activities; as well as posting of our training lectures and video of demonstrations.

国家先进核磁共振技术资源 总体-项目摘要/摘要 核磁共振光谱学是一套独特的实验工具 从大分子复合体到复杂混合物，从原子分辨率，生物学的错综复杂 从结构到动力学在皮秒到千秒的时间尺度上，从化学到功能机制 和动态率。没有其他技术在基础和应用研究方面具有如此广泛的广度和潜力 与其他技术对接，如X射线结晶学、小角X射线散射、冷冻-EM和 还有许多其他的光谱工具。结构表征作为起点，提供了一个框架 以了解可由核磁共振添加的生物活性、作用机制和动力学模型。 核磁共振可以非常好地描述动力学，这可以导致对 蛋白质和其他大分子是如何发挥作用的，复合体是如何形成的，有时是如何动力学的 达到了收费率。复杂混合物的溶液核磁共振波谱已被证明是特别的 可与代谢组学和其他复杂混合物的质谱学结合使用。在这里，我们专注于 关于核磁共振技术的前沿，这是由于最近材料研究和 工具及其实现，面向对追求基本原理感兴趣的广大用户社区 原子分辨率问题是生物医学研究的前沿问题。 三项研发提高了核磁共振的灵敏度，每一项都采用了新技术-第一项是通过使用High 射频线圈的温度超导体，使溶液核磁共振光谱具有独特的灵敏度。Tr&d2 利用磁铁实验室最近安装的600 MHz DNP仪器，该仪器将提供增强的 通过将磁化从电子转移到质子而产生的灵敏度。新的和更强大的DNP 将开发具有扩展温度范围的探头。Tr&d3利用36T系列 用于核磁共振波谱的互联混合-场强跃升超过50%，相当于 从17T(1990年)到23.5T(2016年)的场强发生在过去的26年里！这将导致戏剧性的 提高了灵敏度，信号平均时间甚至显著缩短。科学将会 由一支优秀的DBP团队以及更多跨越非常广泛的科学领域的C&SS团队推动。一个 团队的主要工作将放在培训新一代核磁共振用户上，通过每年一对的 讲习班，并通过出版物和在会议上的发言，通过各种 科学组织和新闻媒体；通过本资源的专用网站和我们的 培训活动；以及张贴我们的培训讲座和演示视频。