TR&D1-HTS

TR

• 批准号: 10217177
• 负责人: William W Brey
• 金额: $ 12.67万
• 依托单位: FLORIDA STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2017
• 资助国家: 美国
• 起止时间: 2017-08-01 至 2023-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10217177
• 关键词: Area; Automobile Driving; Barium; Biological; Complex Mixtures; Copper; Deposition; Detection; Development; Disease; Film; Florida; Frequencies; Gases; Goals; High temperature of physical object; Inosine Diphosphate; Isotope Labeling; Isotopes; Magnetic Resonance Imaging; Measurement; Membrane Proteins; Metabolic; Metabolism; Metals; Methodology; NMR Spectroscopy; Natural Products; Noise; Outcome; Oxides; Pathway interactions; Pattern; Performance; Protein Structure Initiative; Proteins; Reporting; Resources; Saline; Sampling; Sapphire; Scheme; Services; Shapes; Signal Transduction; Structure; System; Techniques; Technology; Testing; Thinness; Universities; Water; Yttrium; base; clinically relevant; cryogenics; design; experimental study; high sensitivity probe; improved; magnetic field; metabolomics; programs; protein structure; structural biology; success; technology development

Project Summary/Abstract TR&D1 The goal of this project is to develop high sensitivity probes based on superconducting resonators for direct detection in metabolomics and in protein structure NMR. For metabolomics, we will develop a probe which is sensitive on both 2H and 13C channels. This probe will be used for isotopomer analysis to assess metabolic changes. For protein structure NMR, the probe will be optimized for efficient detection of 15N. This probe will be designed to exploit the recently described TROSY-based line narrowing effect for 15N at high field, an effect which will be important for structure characterization of intrinsically disordered and membrane- associated proteins. We will also use these probes as technology development projects to improve the performance of superconducting resonators in NMR probes.

项目概要/摘要 TR&D1 该项目的目标是开发基于超导的高灵敏度探针 用于直接检测代谢组学和蛋白质结构 NMR 的谐振器。为了 代谢组学方面，我们将开发一种对 2H 和 13C 通道均敏感的探针。这 探针将用于同位素异构体分析以评估代谢变化。用于蛋白质结构 NMR 探针将针对 15N 的高效检测进行优化。该探测器将被设计为 利用最近描述的基于 TROSY 的高场 15N 线窄化效应，该效应 这对于本质无序和膜结构的结构表征非常重要 相关蛋白质。我们还将使用这些探针作为技术开发项目 提高核磁共振探头中超导谐振器的性能。