SBIR Topic 359 Phase I: Highly-Efficient Isolation and Fidelity Differentiation of Exosomes (HIFiDE) from Bodily Fluids Kit

外泌体包裹并运输由其起源细胞产生的各种分子，这一过程现在被认为是细胞信号传导的一种形式。外泌体信号在细胞类型和物种中都很常见，但在具有炎症成分的疾病中尤其令人感兴趣。虽然外泌体的分离和分析有助于了解这些多方面疾病背后的机制，但外泌体也可能因其治疗潜力而被开发。特别是，来自健康细胞的外泌体已被发现能有效地改变癌细胞的转录组。尽管如此，目前还没有办法区分体液中健康细胞和癌细胞来源的外泌体。在这个项目中，我们的目标是开发一种分离外泌体和癌体的方法，以及一种外泌体群体分化的方法。

Exosomes encapsulate and transport a wide variety of molecules generated by their cell-of-origin, a process now thought to be a form of cellular signaling. Exosome signaling is common across cell types and species, but it is of particular interest in diseases with an inflammatory component. While exosome isolation and analysis is useful to understanding the mechanisms behind these multifaceted diseases, exosomes may also be exploited for their therapeutic potential. In particular, exosomes derived from healthy cells have been found to effectively alter the transcriptome of cancer cells. Nonetheless, there are no methods for differentiating between healthy- and cancer-cell derived exosomes in bodily fluids. In this project, we aim to develop a method for isolating exosomes and oncosomes, coupled with a method for the differentiation of exosome populations.