Project 4: Neural Basis of Behavioral Sequences

项目4：行为序列循环的神经基础 摘要 项目4的目标是了解离散的行动主题是如何组织成目标导向的 在几秒到几分钟的时间尺度上进化的行为序列。行为序列循环是 在我们控制回路层级的最高层，它们整合了高级感官， 表示，并提供下行信号的指导和运动回路。的执行 行为序列需要维持活动的能力（持久性），以及转换的能力 流动性（Fluidity）为了研究持久性和流动性的神经机制，我们将 研究苍蝇大脑中控制顺序行为的回路，如视觉引导行走， 空间导航和工作记忆我们将联合收割机细胞类型特异性遗传驱动系与 体内双光子成像和电生理学，以了解这些电路如何平衡竞争 选择和驱动顺序行为的需求。然后，我们将使用光遗传学工具来测试特定的 在自由行为的苍蝇中产生行为序列的电路模型。这些研究分为 以下三个具体目标： 具体目标1：定义步行随机控制的细胞基础。 具体目标2：识别路径整合背后的神经回路。 具体目标3：研究反转向行为的网络机制。

Project 4: Neural Basis of Behavioral Sequence Loops Abstract The goal of Project 4 is to understand how discrete action motifs are organized into goal-directed behavioral sequences that evolve over timescales of seconds to minutes. Behavioral sequence loops are at the highest level of our control-loop hierarchy, where they integrate high-level sensory representations and provide descending signals to guidance and motor loops. The execution of a behavioral sequence requires the ability to sustain activity (persistence), as well as the ability to switch between behaviors (fluidity). To investigate neural mechanisms of persistence and fluidity, we will study the circuits in the fly brain that control sequential behaviors such as visually guided walking, spatial navigation, and working memory. We will combine cell-type specific genetic driver lines with in vivo 2-photon imaging and electrophysiology to understand how these circuits balance competing demands to select and drive sequential behaviors. We will then use optogenetic tools to test specific circuit models of behavioral sequence generation in freely behaving flies. These studies are divided into the following three Specific Aims: Specific Aim 1: Defining the cellular basis of stochastic control of walking. Specific Aim 2: Identify the neural circuitry underlying path integration. Specific Aim 3: Investigate the network mechanisms of counter-turning behavior.