Chemical editing of nanoscale proteoglycan architecture and organization to control cellular

化学编辑纳米级蛋白聚糖结构和组织以控制细胞

• 批准号: 9753314
• 负责人: Mia L Huang
• 金额: $ 35.7万
• 依托单位: SCRIPPS FLORIDA
• 依托单位国家: 美国
• 财政年份: 2018
• 资助国家: 美国
• 起止时间: 2018-08-01 至 2021-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9753314
• 关键词: Antifreeze; Architecture; Area; Artificial Membranes; Award; Binding; Biochemical; Biology; Catalogs; Cell surface; Cells; Cellular Membrane; Chemicals; Chemistry; Chimera organism; Communities; Complex; Development; Dimensions; Distal; Engineering; Environment; Exhibits; FGF2 gene; Fibroblast Growth Factor; Fibroblasts; Glycobiology; Glycocalyx; Glycoconjugates; Glycosaminoglycans; Glypican; Goals; Grant; Growth; Growth Factor; Interdisciplinary Study; Laboratories; Length; Mediating; Medicine; Membrane; Membrane Microdomains; Mentors; Methods; Molecular Biology; Molecular Biology Techniques; Monitor; Muscle Cells; Muscle Fibers; Musculoskeletal; Myoblasts; Natural regeneration; Oligosaccharides; Outcome; Pattern; Phase; Phenotype; Play; Polymers; Polysaccharides; Positioning Attribute; Postdoctoral Fellow; Protein Engineering; Proteoglycan; Regenerative Medicine; Research; Scholarship; Signal Transduction; Site; Skeletal Muscle; Specific qualifier value; Specificity; Stem cells; Structure; Structure-Activity Relationship; Surface; Techniques; Technology; Time; Training; Universities; Unspecified or Sulfate Ion Sulfates; Work; antimicrobial; biophysical techniques; career; career development; cellular development; collaborative environment; design; embryonic stem cell; graduate student; innovation; laboratory experience; mimetics; myogenesis; nanomaterials; nanoscale; nanoscience; neuroregulation; peptidomimetics; professor; proteoglycan core protein; receptor; regenerative; relating to nervous system; research and development; scaffold; skills; stem cell differentiation; sulfation; syndecan; tool

PROJECT SUMMARY/ABSTRACT Research: Cellular development is orchestrated by cell surface proteoglycans. Despite their potential for biomedicine, strategies that harness their functions are limited. This gap is caused by the lack of techniques that recognize their nanoscale complexity. This application outlines the development of chemical strategies to mimic and probe the nanoscale architecture and organization of proteoglycans in cellular development. In two specific aims, we will demonstrate that the techniques developed through this proposal will enable new chemical strategies to control embryonic stem cell differentiation and the regeneration of skeletal muscles. Career Goals and Background. My long-term goal is to become a professor at a major University within US. In this position, I intend to establish a research platform that encompasses the fields of chemistry, glycobiology, and nanoscience to advance biomedicine. The two aspects embedded into the K99/R00 platform will allow me to achieve my goal. Throughout my graduate and postdoctoral career, I have demonstrated my capabilities for interdisciplinary research at the interface of chemistry, nanoscience, and engineering. As a graduate student, I developed peptidomimetics molecules that exhibit antimicrobial or antifreeze activity. These projects have enabled me to be well-versed in chemical biology and biophysical techniques to characterize membranes. As a postdoctoral fellow, I have acquired skills in polymer synthesis and stem cell differentiation. Thus, my academic and research background makes me capable of achieving the goals of this proposal within the specified time frames. Training and Environment: UC San Diego has a superb academic and scientific community that facilitates a highly innovative and collaborative environment, which will promote my scholarship and professional growth. The mentored phase of this Award will allow me to acquire new skills in molecular biology, glycobiology and professional development that will be critical for my transition to an independent career. The proposed scientific training will be conducted with the tutelage of my mentor and co-mentor, Prof. Kamil Godula and Prof. Jeffrey Esko, who are pioneers in glycopolymer technology, molecular biology, and glycobiology. These new skills will be augmented by a mixture of didactic and hands-on laboratory training. I will have direct access to all the necessary components of my research and career development. There is a wealth of professional development activities within UC San Diego that will enable me to become a better grant writer and manager. I have also assembled a world-class mentoring committee who will monitor and advise on my research progress and transition into an independent career.

项目总结/摘要 研究：细胞发育是由细胞表面蛋白聚糖协调的。尽管它们有潜力 在生物医学中，利用其功能的策略是有限的。这种差距是由于缺乏技术造成的 认识到它们纳米级的复杂性。本申请概述了化学战略的发展， 模拟和探测细胞发育中蛋白聚糖的纳米级结构和组织。在两 具体目标，我们将证明，通过这项建议开发的技术将使新的 控制胚胎干细胞分化和骨骼肌再生的化学策略。 职业目标和背景。我的长期目标是成为一所重点大学的教授， 我们在这个职位上，我打算建立一个研究平台，包括化学领域， 糖生物学和纳米科学来推进生物医学。嵌入K99/R 00平台的两个方面 能让我实现目标在我的研究生和博士后生涯中，我证明了我的 在化学，纳米科学和工程的接口跨学科研究的能力。作为 作为一名研究生，我开发了具有抗菌或防冻活性的肽模拟物分子。这些 项目使我能够精通化学生物学和生物物理技术， 膜。作为博士后研究员，我获得了聚合物合成和干细胞分化方面的技能。 因此，我的学术和研究背景使我有能力实现这一建议的目标， 特定的时间框架。 培训和环境：UC San Diego拥有一流的学术和科学社区， 促进了高度创新和协作的环境，这将促进我的奖学金， 专业成长。该奖项的指导阶段将使我获得分子生物学的新技能， 糖生物学和专业发展，这将是我过渡到一个独立的职业生涯的关键。的 我将在我的导师和共同导师卡米尔教授的指导下进行拟议的科学培训 Godula和Jeffrey Esko教授，他们是糖聚合物技术，分子生物学和 糖生物学这些新技能将通过教学和动手实验室培训的混合来增强。我 将直接接触到我的研究和职业发展的所有必要组成部分。有一个 在加州大学圣地亚哥分校的丰富的专业发展活动，这将使我成为一个更好的赠款 作家兼经理我还组建了一个世界级的指导委员会， 我的研究进展和向独立职业的过渡。