Nanoparticle-driven systems for tunable local pain relief

总结 用于长时间或可耐受局部麻醉的药物输送系统的研究总是 包括在体外和体内测量药物释放到液体中。在这两种情况下，但 特别是在体内，药物可能以非常低的浓度存在-太低而不能被 通过色谱法检测。同样在这两种情况下，可能存在感兴趣的药物 与其他可能混淆药物检测和定量的化合物。这 这个问题通常用液相色谱-质谱法（LC-MS）来克服。LC- MS还可用于真实的时间中的化学反应监测，以确定我们是否获得 用于持续和/或触发释放系统的所需产品，并优化化学品 合成路线LC-MS是我们在研究中经常使用的技术， 黑鬼。进入LC-MS一直是我们研究的严重瓶颈，因为只有一个 我们的机构已经严重超额认购了使用其他机构的LC-MS是昂贵的，时间- 消耗（行程），样品稳定性欠佳，真实的时间反应不切实际 监测.因此，我们正在申请行政补充购买LC-MS 为我们的实验室。

Summary The study of drug delivery systems for prolonged or triggerable local anesthesia invariably involves measuring the release of drugs into fluids, in vitro and in vivo. In both cases, but particularly in vivo, the drugs may be present at very low concentrations – too low to be detected by chromatographic means. Also in both cases, the drugs of interest may be present with other compounds that can confound the detection and quantitation of the drug. This problem is commonly overcome with liquid chromatography-mass spectrometry (LC-MS). LC- MS is also useful for chemical reaction monitoring in real time to determine whether we obtain the desired product for sustained and/or triggered release systems and to optimize the chemical synthesis routes. LC-MS is a technique that we have frequently used in our research funded by NIGMS. Access to LC-MS has been a serious bottleneck to our research since there is only one at our institution and it is heavily oversubscribed. Using other institutions' LC-MS is costly, time- consuming (travel), suboptimal for sample stability, and impractical for real time reaction monitoring. We are therefore applying for an administrative supplement to purchase an LC-MS for our lab.