Comprehensive Biology: Exploiting the Yeast Genome

项目摘要 自成立以来，酵母资源中心一直致力于了解基因组序列如何与 to protein蛋白质function功能.该中心成立之际，芽殖酵母成为第一个拥有基因组的真核生物 序列完成，我们建议使用一系列技术来解释这个序列。在这 应用，我们承担新的挑战，集中在了解蛋白质的变化如何影响其 层次、修饰、功能和结构。我们将在三个方面开发新技术：1）扰动和 传感复杂途径的变化; 2）通过质谱法进行蛋白质检测和定量;和3） 高级蛋白质结构。这些技术将由十个紧密结合的驱动生物医学 项目酵母仍然是开发、测试和改进这些技术的无与伦比的实验系统。 随着技术的成熟，我们将通过内部项目将其应用扩展到高等真核生物， 外部合作。此外，我们通过以下方式， 广泛的合作以及良好的传播和培训记录。

Project Summary Since its inception, the Yeast Resource Center has focused on understanding how genome sequence relates to protein function. The Center began just as budding yeast became the first eukaryote to have its genome sequence completed, and we proposed to use an array of technologies to interpret this sequence. In this application, we undertake novel challenges, centered on understanding how variation in proteins affects their levels, modification, function and structure. We will develop new technologies in three areas: 1) Perturbing and sensing changes to complex pathways; 2) Protein detection and quantitation by mass spectrometry; and 3) Higher order protein structure. These technologies will be driven by ten closely integrated Driving Biomedical Projects. Yeast remains an unparalleled experimental system to develop, test and refine these technologies. As the technologies mature, we will extend their application to higher eukaryotes through internal projects and external collaborations. Furthermore, we make our technologies available as they become robust, through broad collaboration and a well-established track record of dissemination and training.