Comprehensive Biology: Exploiting the Yeast Genome

综合生物学：利用酵母基因组

• 批准号: 9900797
• 负责人: Michael MacCoss
• 金额: $ 187.07万
• 依托单位: UNIVERSITY OF WASHINGTON
• 依托单位国家: 美国
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-09-30 至 2022-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9900797
• 关键词: Affect; Area; Automobile Driving; Beds; Biology; Cell physiology; Cells; Collaborations; Communities; Complex; Data; Data Scientist; Detection; Development; Educational workshop; Eukaryota; Foundations; Genome; Goals; Grant; Human Resources; Infrastructure; Laboratories; Learning; Link; Mass Spectrum Analysis; Measurement; Methods; Modification; Pathway interactions; Play; Proteins; Publications; Recording of previous events; Research; Research Personnel; Resource Sharing; Resources; Role; Saccharomycetales; Scientist; Site; Source; Structure; System; Technology; Testing; Training; Training Activity; Training and Infrastructure; Variant; Visit; Work; Yeasts; data resource; design; improved; meetings; new technology; novel; protein function; protein structure; software development; technology development; technology research and development; tool; training opportunity; web site; yeast genome

Project Summary Since its inception, the Yeast Resource Center has focused on understanding how genome sequence relates to protein function. The Center began just as budding yeast became the first eukaryote to have its genome sequence completed, and we proposed to use an array of technologies to interpret this sequence. In this application, we undertake novel challenges, centered on understanding how variation in proteins affects their levels, modification, function and structure. We will develop new technologies in three areas: 1) Perturbing and sensing changes to complex pathways; 2) Protein detection and quantitation by mass spectrometry; and 3) Higher order protein structure. These technologies will be driven by ten closely integrated Driving Biomedical Projects. Yeast remains an unparalleled experimental system to develop, test and refine these technologies. As the technologies mature, we will extend their application to higher eukaryotes through internal projects and external collaborations. Furthermore, we make our technologies available as they become robust, through broad collaboration and a well-established track record of dissemination and training.

项目摘要 自成立以来，酵母资源中心一直致力于了解基因组序列如何与 to protein蛋白质function功能.该中心成立之初，芽殖酵母刚刚成为第一个拥有基因组的真核生物 序列完成，我们建议使用一系列技术来解释这个序列。在这 应用，我们承担新的挑战，集中在了解蛋白质的变化如何影响其 层次、修饰、功能和结构。我们将在三个方面开发新技术：1）扰动和 感测复杂途径的变化; 2）通过质谱法进行蛋白质检测和定量;以及3） 高级蛋白质结构。这些技术将由十个紧密结合的驱动生物医学 项目酵母仍然是开发、测试和完善这些技术的无与伦比的实验系统。 随着技术的成熟，我们将通过内部项目将其应用扩展到高等真核生物， 外部合作。此外，我们通过以下方式， 广泛的合作以及良好的传播和培训记录。