Genomics and Imaging Facilities Core

基因组学和成像设施核心

• 批准号: 9913528
• 负责人: Stuart S Levine
• 金额: $ 17.46万
• 依托单位: MASSACHUSETTS INSTITUTE OF TECHNOLOGY
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至 2023-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9913528
• 关键词: Address; Adoption; Area; Bioinformatics; Biological; Biological Assay; Biological Sciences; Biology; Cell physiology; Cells; Chromatin; Collaborations; Computer software; Consultations; Core Facility; Custom; DNA Microarray Chip; Data; Emerging Technologies; Engineering; Environment; Environmental Health; Environmental Impact; Equipment; Fee-for-Service Plans; Flow Cytometry; Funding; Genomics; Human; Image; Imaging technology; Infrastructure; Institutes; Investments; Joints; Libraries; Liquid substance; Massive Parallel Sequencing; Microscopy; Mission; Modeling; Nature; Oligonucleotide Microarrays; Preparation; Procedures; Process; Quality Control; Reader; Reading; Research Personnel; Resolution; Resource Sharing; Resources; Sampling; Services; Source; Systems Biology; Technology; Time; Training; Work; anticancer research; bioinformatics resource; data modeling; data quality; equipment acquisition; high resolution imaging; high throughput screening; imaging capabilities; imaging facilities; instrument; instrumentation; large datasets; member; microbiome; new technology; open source; protein expression; tool

GENOMICS AND IMAGING FACILITIES CORE: PROJECT SUMMARY/ABSTRACT The CEHS Genomics and Imaging Facilities Core (GIFC) provides CEHS members with a broad set of instrumentation, training and expertise to address environmental health using systems biology approaches. This Core provides access to massively parallel sequencing, oligonucleotide arrays, automated fluidics, microscopy and bioinformatics and IT support. Over the previous funding period, this Core has expanded significantly with over $3 million in additional equipment and a significant increase in space allocation. These expansions include the acquisition of several Illumina sequencers and significant investments in bioinformatics and bioIT. The GIFC is comprised of the CEHS Imaging Facility, overseen by Dr. Robert Croy and the MIT BioMicro Center, a joint institutional shared resource that provides the genomics and bioinformatics resources in the core, overseen by Dr. Stuart Levine. The BioMicro Center is the central genomics and bioinformatics technology resource at MIT and it is jointly supported by the CEHS, the Departments of Biological Engineering and Biology, and the David H. Koch Institute for Integrative Cancer Research. While over half of all CEHS members have utilized the GIFC in the current funding period, the CEHS only provides 5% of the support for the Facilities Core, and members have benefitted extensively from equipment donated from a broad variety of sources including charitable and institutional support. The GIFC activities can be divided into four major areas of technology for which the core provides both expertise and training. Genomics assays, including Affymetrix and Agilent microarrays, Illumina and Pacific Biosciences sequencing and library construction, offer CEHS members the assay a broad variety of questions from how cells respond to environmental challenges to how the environment impacts the microbiome. High- throughput assays, including qPCR, plate readers and automated liquid handling allow CEHS members to significantly increase sample throughput. Microscopy, including flow-cytometry and the Cellomics Arrayscan, provide assays of changes in protein expression and localization, and bioinformatics and bioIT provide both infrastructure as well as assistance in the analyses of these large datasets. Services are offered as walkup services where the investigator will directly operate the instrument following training, fee-for-service services, where samples are processed or analyzed for the user on highly specialized equipment by the GIFC staff and instruments that can be operated in either way.

基因组学和成像设施核心：项目摘要/摘要 CEHS 基因组学和成像设施核心 (GIFC) 为 CEHS 成员提供了广泛的 利用系统生物学方法解决环境健康问题的仪器、培训和专业知识。 该核心提供了大规模并行测序、寡核苷酸阵列、自动流体学、 显微镜、生物信息学和 IT 支持。在上一个资助期间，该核心已扩大 显着增加了超过 300 万美元的额外设备，并显着增加了空间分配。这些 扩张包括收购数台 Illumina 测序仪以及对生物信息学的重大投资 和生物信息技术。 GIFC 由 CEHS 成像设施组成，由 Robert Croy 博士和麻省理工学院 BioMicro 监督 中心，一个联合机构共享资源，提供基因组学和生物信息学资源 核心，由 Stuart Levine 博士监督。 BioMicro 中心是基因组学和生物信息学的中心 麻省理工学院的技术资源，并得到 CEHS、生物工程系的联合支持 和生物学，以及大卫·H·科赫综合癌症研究所。虽然超过一半的 CEHS 成员在当前资助期内使用了GIFC，CEHS仅提供5%的支持 设施核心，成员从各种捐赠的设备中受益匪浅 来源包括慈善和机构支持。 GIFC 活动可分为四个主要技术领域，其核心提供了 专业知识和培训。基因组学检测，包括 Affymetrix 和 Agilent 微阵列、Illumina 和 Pacific 生物科学测序和文库构建为 CEHS 成员提供了多种分析问题 从细胞如何应对环境挑战到环境如何影响微生物组。高的- 通量测定，包括 qPCR、读板器和自动液体处理，使 CEHS 成员能够 显着提高样品通量。显微镜，包括流式细胞术和 Cellomics Arrayscan， 提供蛋白质表达和定位变化的分析，生物信息学和生物IT提供两者 基础设施以及分析这些大型数据集的帮助。服务以无预约形式提供 研究者在培训后直接操作仪器的服务、按服务收费的服务、 GIFC 工作人员在高度专业化的设备上为用户处理或分析样品，并且 可以以任一方式操作的仪器。