Genomics and Imaging Facilities Core

基因组学和成像设施核心

• 批准号: 9913528
• 负责人: Stuart S Levine
• 金额: $ 17.46万
• 依托单位: MASSACHUSETTS INSTITUTE OF TECHNOLOGY
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至 2023-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9913528
• 关键词: Address; Adoption; Area; Bioinformatics; Biological; Biological Assay; Biological Sciences; Biology; Cell physiology; Cells; Chromatin; Collaborations; Computer software; Consultations; Core Facility; Custom; DNA Microarray Chip; Data; Emerging Technologies; Engineering; Environment; Environmental Health; Environmental Impact; Equipment; Fee-for-Service Plans; Flow Cytometry; Funding; Genomics; Human; Image; Imaging technology; Infrastructure; Institutes; Investments; Joints; Libraries; Liquid substance; Massive Parallel Sequencing; Microscopy; Mission; Modeling; Nature; Oligonucleotide Microarrays; Preparation; Procedures; Process; Quality Control; Reader; Reading; Research Personnel; Resolution; Resource Sharing; Resources; Sampling; Services; Source; Systems Biology; Technology; Time; Training; Work; anticancer research; bioinformatics resource; data modeling; data quality; equipment acquisition; high resolution imaging; high throughput screening; imaging capabilities; imaging facilities; instrument; instrumentation; large datasets; member; microbiome; new technology; open source; protein expression; tool

GENOMICS AND IMAGING FACILITIES CORE: PROJECT SUMMARY/ABSTRACT The CEHS Genomics and Imaging Facilities Core (GIFC) provides CEHS members with a broad set of instrumentation, training and expertise to address environmental health using systems biology approaches. This Core provides access to massively parallel sequencing, oligonucleotide arrays, automated fluidics, microscopy and bioinformatics and IT support. Over the previous funding period, this Core has expanded significantly with over $3 million in additional equipment and a significant increase in space allocation. These expansions include the acquisition of several Illumina sequencers and significant investments in bioinformatics and bioIT. The GIFC is comprised of the CEHS Imaging Facility, overseen by Dr. Robert Croy and the MIT BioMicro Center, a joint institutional shared resource that provides the genomics and bioinformatics resources in the core, overseen by Dr. Stuart Levine. The BioMicro Center is the central genomics and bioinformatics technology resource at MIT and it is jointly supported by the CEHS, the Departments of Biological Engineering and Biology, and the David H. Koch Institute for Integrative Cancer Research. While over half of all CEHS members have utilized the GIFC in the current funding period, the CEHS only provides 5% of the support for the Facilities Core, and members have benefitted extensively from equipment donated from a broad variety of sources including charitable and institutional support. The GIFC activities can be divided into four major areas of technology for which the core provides both expertise and training. Genomics assays, including Affymetrix and Agilent microarrays, Illumina and Pacific Biosciences sequencing and library construction, offer CEHS members the assay a broad variety of questions from how cells respond to environmental challenges to how the environment impacts the microbiome. High- throughput assays, including qPCR, plate readers and automated liquid handling allow CEHS members to significantly increase sample throughput. Microscopy, including flow-cytometry and the Cellomics Arrayscan, provide assays of changes in protein expression and localization, and bioinformatics and bioIT provide both infrastructure as well as assistance in the analyses of these large datasets. Services are offered as walkup services where the investigator will directly operate the instrument following training, fee-for-service services, where samples are processed or analyzed for the user on highly specialized equipment by the GIFC staff and instruments that can be operated in either way.

基因组学和成像设施核心：项目摘要/摘要 CEHS基因组学和成像设施核心（GIFC）为CEHS成员提供了广泛的 使用系统生物学方法来解决环境健康的仪器，培训和专业知识。 该核心可访问大量并行测序，寡核苷酸阵列，自动流体，， 显微镜和生物信息学及其支持。在过去的资金期间，此核心已扩大 显着的额外设备超过300万美元，太空分配的大幅增加。这些 扩展包括收购几个Illumina测序仪以及对生物信息学的大量投资 和生物。 GIFC由CEHS成像设施组成，由Robert Croy博士和MIT BioMicro监督 中心是一种共同的机构共享资源，可提供基因组学和生物信息学资源 核心，由Stuart Levine博士监督。生物学中心是中心基因组学和生物信息学 麻省理工学院的技术资源，并由CEHS共同支持生物工程部门 和生物学，以及大卫·科赫（David H. Koch）综合癌症研究所。而CEH的一半以上 成员在当前的资金期间使用了GIFC，CEHS仅提供5％的支持 设施核心和成员从各种各样的设备中受益匪浅 包括慈善和机构支持在内的资料。 GIFC活动可以分为四个主要技术领域 专业知识和培训。基因组学测定，包括Affymetrix和Agilent微阵列，Illumina和Pacific 生物科学测序和图书馆建设，为CEHS成员提供分析的各种问题 从细胞对环境挑战的反应到环境如何影响微生物组。高的- 吞吐量测定，包括QPCR，板块读取器和自动液体处理，使CEHS成员得以 显着增加样品吞吐量。显微镜，包括流程度仪和大提琴学阵列， 提供蛋白质表达和定位变化的测定，生物信息学和生物IT既提供 基础架构以及对这些大数据集的分析的帮助。服务作为步行 在培训后，调查人员将直接操作该工具的服务，费用服务服务， GIFC员工和 可以以两种方式操作的仪器。