Expanding access to Genomics Methods through Modern Focused Ultrasonication

通过现代聚焦超声扩大基因组学方法的应用

• 批准号: 10735363
• 负责人: Stuart S Levine
• 金额: $ 12.88万
• 依托单位: MASSACHUSETTS INSTITUTE OF TECHNOLOGY
• 依托单位国家: 美国
• 财政年份: 2023
• 资助国家: 美国
• 起止时间: 2023-07-01 至 2024-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10735363
• 关键词: Acoustics; Address; Area; Biological; Biological Assay; Biomedical Research; Cells; Chemistry; Chromatin; Complement; Complex; Coupled; Cytolysis; DNA; Deposition; Epigenetic Process; Equipment; Expenditure; Experimental Designs; Faculty; Funding; Future; Genomics; Genomics Shared Resource; Human Development; Laboratories; Libraries; Mechanics; Methodology; Methods; Modernization; Nucleic acid sequencing; Performance; Plastics; Preparation; Protocols documentation; RNA; RNA purification; Reproducibility; Research; Research Personnel; Research Project Grants; Resource Sharing; Resources; Sampling; Site; Sonication; Source; Technology; Tissues; Transducers; Update; Work; bioinformatics resource; biological research; data management; epigenomics; experimental study; genome resource; human disease; improved; instrument; instrumentation; novel; nucleic acid purification; prevent; success; timeline; transcriptome sequencing; transcriptomics

SUMMARY The MIT BioMicro Center serves as a centralized resource for MIT researchers to access genomic and bioinformatics resources as well as expertise in related methodologies. As an integrated resource, the BioMicro Center is able to support researchers over the entire timeline of their experiment with assistance available in experimental design, sample extraction and preparation, sequencing, analysis, and deposition and data management. The facility supports over 120 laboratories each year and approximately $4.2m of funds in total research expenditure. The large diversity of research projects supported by the BioMicro Center creates unique challenges for the shared resource. While many genomics shared resources specialize in specific assays or focus on a small number of species, no specific methodology represents a disproportionate fraction of the work supported by the center. Improvements in sequencing capacity and the success of the BioMicro Center in developing novel protocols to prepare nucleic acids for sequencing has exposed a number of critical barriers that are limiting researchers at MIT, preventing them from taking full advantage of new methods as well as constraining other areas of biological and biomedical research. Foremost among these is a lack of high-throughput processing of biological samples for extraction and purification of nucleic acids. Equipment requested in this proposal, a Covaris R230 Focused Ultrasonicator, will modernize the Center’s ability to isolate biomolecules from a broad spectrum of biological sources, and to prepare those molecules for genomic, transcriptomic, and epigenomic analysis using state-of-the-art sequencing technologies. The new equipment will do so by impacting the workflows in the BioMicro Center in three ways. First, it will enable high-throughput RNAseq of degraded samples. Second, it will enhance high-throughput preparation of diverse samples including FFPE tissue. Third, novel plastics used in the instrument will improve epigenetic assay access and performance. The Covaris R230 will complement the existing portfolio of instrumentation within the BioMicro Center, leveraging that equipment to enable faculty across several research centers at MIT to routinely ask highly powered questions to address challenging biological and biomedical problems. Access to high-throughput and updated sonication will expand and enhance the capabilities of the Center to support existing research projects, improving their rigor and reproducibility, while enabling future projects to leverage the instrumentation to ask broader questions due to the ability to easily handle a larger spectrum of biospecimens.

概括 麻省理工学院BioMicro Center是麻省理工学院研究人员访问的集中资源 基因组和生物信息学资源以及相关方法方面的专业知识。作为 综合资源，BioMicro中心能够在整个时间表中支持研究人员 在实验设计，样品提取和 准备，测序，分析，沉积和数据管理。设施支持 每年超过120多个实验室，总研究的资金约为420万美元 支出。 BioMicro Center支持的大量研究项目创造了 共享资源的独特挑战。虽然许多基因组学共享资源专业 在特定的测定或专注于少量规格中，没有特定方法代表 中心支持的工作的不成比例。测序的改进 能力和生物学中心在制定新方案准备准备的能力和成功 用于测序的核酸暴露了许多限制的关键障碍 麻省理工学院的研究人员，阻止他们充分利用新方法以及 限制生物和生物医学研究的其他领域。其中最重要的是缺乏 生物样品的高通量加工以提取和纯化核能 酸。该提案中要求的设备是Covaris R230专注的超声波，将 现代化该中心将生物分子与广泛生物学分离的能力现代化 来源，并准备这些分子进行基因组，转录组和表观基因组分析 使用最先进的测序技术。新设备将通过影响 生物学中心的工作流以三种方式。首先，它将启用高通量RNASEQ 降级样品。其次，它将增强各种样品的高通量准备 包括FFPE组织。第三，仪器中使用的新型塑料将改善表观遗传测定 访问和性能。 Covaris R230将完成现有的投资组合 生物学中心内的仪器，利用该设备使教职员工跨过 麻省理工学院的几个研究中心通常提出高电力的问题以解决 具有挑战性的生物学和生物医学问题。访问高通量和更新 超声处理将扩大和增强中心支持现有研究的能力 项目，改善其严格性和可重复性，同时使未来的项目能够利用 由于能够轻松处理更大范围 生物测量。