Genomics Facilities Core

基因组学设施核心

• 批准号: 8830354
• 负责人: Stuart S Levine
• 金额: $ 24.63万
• 依托单位: MASSACHUSETTS INSTITUTE OF TECHNOLOGY
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/8830354
• 关键词: Bioinformatics; Biological; Cell physiology; Computer software; Core Facility; Coupled; Data; Data Quality; Data Set; Development; Devices; Environmental Health; Environmental Impact; Environmental Risk Factor; Evolution; Fluorescence; Genomics; Image; Imaging technology; Laboratories; Massive Parallel Sequencing; Modeling; Oligonucleotide Microarrays; Procedures; Quality Control; Research; Resolution; Resources; Services; System; Technology; Time; Training; Work; analytical method; cellular imaging; data mining; data modeling; data sharing; flexibility; high throughput screening; imaging modality; large-scale database; luminescence; member; new technology; open source; response; technology development; tool

7C. GENOMICS AND IMAGING FACILITIES CORE INTRODUCTION AND SPECIFIC AIMS The CEHS Genomics and Bioinformatics Facilities Core was created in September 2001 to provide CEHS members with integrated facilities for high-throughput, data-intensive genomics as well as bioinformatics analysis, large-scale database storage and management, data mining and data modeling required to fully implement systems approaches to studying the biological impact of environmental factors. In response to the evolution of Center member research needs, a new CEHS Imaging Facility was integrated with the CEHS Genomics and Bioinformatics Facilities Core last year, and this CEHS facilities core has been renamed the "Genomics and Imaging Facilities Core." The Core now provides CEHS members with the tools and expertise for high-resolution and high-throughput imaging integrated with data-intensive genomics and bioinformatics. This powerful combination is coupled with flexible and accessible data sharing and a strong commitment to new technology development and deployment to keep the CEHS at the cutting edge of high-throughput imaging and analytical methods. The Genomics and Imaging Facilities Core operates as both a training resource and as a service and developmental laboratory, with the following Specific Aims: (1) To provide integrated support for genomics technologies including massively parallel sequencing and oligonucleotide arrays. (2) To provide customized imaging options and the resources for high-resolution images and high-throughput screening using imaging capabilities. (3) To provide integrative support for high-throughput screening of factors with environmental impact on cellular function, including support for flexible automated fluidics, highly parallel real-time PCR, fluorescence, luminescence, absorbance, and single-cell imaging and quantification. (4) To support the bioinformatic frameworks necessary to interpret and manage these large data sets. (5) To provide and maintain network servers and storage devices to facilitate the transfer and analysis of information-rich data sets. (6) To provide training with new genomic and imaging technologies as well as commercial and open-source bioinformatics software. (7) To work with Center members to develop and acquire new technologies and to apply their results to develop data models and quality control procedures and error modeling.

7C.基因组学和成像设施核心 导言和具体目标 CEHS基因组学和生物信息学设施核心创建于2001年9月，为CEHS成员提供高通量，数据密集型基因组学以及生物信息学分析，大规模数据库存储和管理，数据挖掘和数据建模所需的综合设施，以全面实施系统方法来研究环境因素的生物影响。为了满足中心成员研究需求的变化，去年，一个新的CEHS成像设施与CEHS基因组学和生物信息学设施核心相结合，该CEHS设施核心已更名为“基因组学和成像设施核心”。“核心现在为CEHS成员提供高分辨率和高通量成像的工具和专业知识，并将其与数据密集型基因组学和生物信息学相结合。这种强大的组合加上灵活和可访问的数据共享以及对新技术开发和部署的坚定承诺，使CEHS保持在高通量成像和分析方法的最前沿。基因组学和成像设施核心既作为培训资源，也作为服务和发展实验室，具有以下具体目标： (1)为基因组学技术提供综合支持，包括大规模平行测序和寡核苷酸阵列。 (2)提供定制的成像选项和资源，用于高分辨率图像和使用成像功能的高通量筛选。 (3)为高通量筛选对细胞功能有环境影响的因子提供综合支持，包括支持灵活的自动化射流、高度并行的实时PCR、荧光、发光、吸光度以及单细胞成像和定量。 (4)支持解释和管理这些大型数据集所需的生物信息学框架。 (5)提供和维护网络服务器和存储设备，以促进信息丰富的数据集的传输和分析。 (6)提供新的基因组和成像技术以及商业和开源技术的培训 生物信息学软件 (7)与中心成员合作开发和获取新技术，并将其成果应用于开发数据模型和质量控制程序以及错误建模。