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CSHL Advanced Techniques in Molecular Neuroscience Course

CSHL 分子神经科学高级技术课程

基本信息

批准号：
10355515
负责人：
DAVID J. STEWART
金额：
$ 12.86万
依托单位：
COLD SPRING HARBOR LABORATORY
依托单位国家：
美国
项目类别：
财政年份：
2015
资助国家：
美国
起止时间：
2015-07-06 至 2025-02-28
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10355515
关键词：
ATAC-seq Applied Research Area Attention Bacterial Artificial Chromosomes Basic Science Binding Biological Assay Cell Culture Techniques Chromatin Clustered Regularly Interspaced Short Palindromic Repeats Collaborations Communities Course Content Detection Disease Electrophysiology (science)Engineering Ensure Environment Exercise Faculty Female Funding Future Home Image Immunoprecipitation Individual Institution Laboratories Mainstreaming Mental Health Messenger RNA Methodology Methods Molecular Molecular Neurobiology Neurobiology Neurosciences Neurosciences Research New York Participant Pharmacology Physicians Postdoctoral Fellow Preparation Process Publications RNA RNA Binding Reaction Reporting Research Research Design Research Personnel Role Running Scientist Series Specific qualifier value Students Techniques Time Tissues Training Training Programs Transgenic Organisms Translating Underrepresented Minority United States Update Viral Vector astrocyte progenitor base design experience forging gene delivery system genome editing genome-wide graduate student in vivo instructor interest laboratory curriculum laboratory manuals lecturer medical specialties member mid-career faculty nerve stem cell nervous system disorder online resource programs protein crosslink recruit relating to nervous system tool transcription factor vector work-study

项目摘要

ABSTRACT CSHL Laboratory Course in Advanced Techniques in Molecular Neuroscience The proposed Cold Spring Harbor Laboratory (CSHL) course Advanced Techniques in Molecular Neuroscience (ATMN), to be held annually in July 2020– 2024, is part of the CSHL's postgraduate training program in neurobiology. ATMN is a short, intensive sixteen day hands-on course which prepares students to enter directly into research that makes use of advanced and/or specialized molecular techniques in contemporary aspects of neurobiological research. Experimental modules in the course will include quantitative assays of RNAs bound to protein by crosslinking immunoprecipitation (CLIP), translating mRNA isolation from specified neural subtypes (TRAP), tissue clearing methodologies combined with single RNA molecule detection by hybridization chain reaction (HCR), preparation of pure astrocyte and neural progenitor cell cultures, design and use of engineered viral vectors in neuroscience research, design and use of CRISPR/Cas genome editing tools, practical exercises in gene delivery systems in vivo, genome-wide assessment of chromatin state and transcription factor binding by ATACseq and Cut & Run, and use and design of BAC transgenic vectors. Experimental techniques are taught in the context of the broader conceptual advances that they may provide. The course invites lecturers who have made significant contributions in their fields to give up-to-the-minute reports on current research. The trainees are chosen by the course faculty from larger pools of applicants and range from graduate students to senior investigators and research physicians. Because of the short duration of this course, senior, as well as junior individuals can attend and receive a short, intense period of training in an environment remote from other demands on their time and attention. The ATMN course also provides an unusual opportunity for established scientists to apply these advanced techniques to their own research interests, or to retrain in an important specialty within neuroscience with which they may not be familiar. Methods and concepts taught in the course are disseminated to the wider research community through the publication of laboratory manuals and online resources.

摘要