Antibody isotyping for discrimination of disease stage and diagnosis of early Lyme disease.
用于区分疾病阶段和诊断早期莱姆病的抗体同种型。
基本信息
- 批准号:10204992
- 负责人:
- 金额:$ 29.55万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2020
- 资助国家:美国
- 起止时间:2020-07-01 至 2023-03-31
- 项目状态:已结题
- 来源:
- 关键词:AcuteAcute DiseaseAffinityAntibodiesAntibody ResponseAntigensArthritisBenchmarkingBiological AssayBiological MarkersBorrelia burgdorferiCenters for Disease Control and Prevention (U.S.)ClinicClinicalCollaborationsDataDevelopmentDiagnosisDiagnosticDiagnostic SpecificityDiscriminationDiseaseEarly DiagnosisEnzyme ImmunoassayEvaluationGTP-Binding Protein alpha Subunits, GsGenetic RecombinationGoalsGrantHigh PrevalenceHumanIgA1IgA2IgEIgG1IgG2IgG3IgG4Immune responseImmunodominant AntigensImmunoglobulin AImmunoglobulin DImmunoglobulin GImmunoglobulin IsotypesImmunoglobulin MImmunoglobulinsInfectionIowaLaboratoriesLaboratory DiagnosisLesionLettersLicensingLyme ArthritisLyme DiseaseMachine LearningOspC proteinPatientsPeptidoglycanPerformancePhaseProteinsROC CurveRecommendationReproducibilityResearchSerumSmall Business Innovation Research GrantSpecificityTechniquesTechnologyTestingTimeTrainingUniversitiesUpdateV(D)J RecombinationVisualWorkantigen bindingbasecommercializationdisease diagnosiserythema migransfollow-upimprovedlateral flow assaynovel diagnosticspathogentool
项目摘要
ABSTRACT
More than 3 million tests are performed each year to support the laboratory diagnosis of human Lyme
disease (LD). While the CDC conventional standard two-tier (CSTT) approach for serodiagnosis of
LD has worked relatively well when used as recommended, there is plenty of room for improvement.
Of a number of weaknesses associated with the supplemental immunoblot of the CSTT the most
significant is low reproducibility due to the subjective visual interpretation of results. To overcome
these weaknesses the CDC recently updated its recommendations based on a modified STT (MSTT)
in that a second EIA can replace the immunoblot. The major goal of this project is to develop an
objective, quantitative, multiplex EIA that can detect four antibody isotypes (IgM/D/G/A) and all four
IgG subclasses (IgG1/2/3/4) to leverage acquisition of simultaneous antibody profile information on
multiple B. burgdorferi antigens to build an assay that can discriminate Lyme disease stage with
increased overall sensitivity without incurring in loss of specificity. The novelty of this study relies on:
1) evaluation of B. burgdorferi antigen-specific antibody isotypes and IgG subclasses that can be
correlated with Lyme disease stage; and 2) development of new diagnostic tools using machine
learning techniques to train and integrate all data and produce an objective result to discriminate early
Lyme from early disseminated/late Lyme disease. We expect this Phase I SBIR to allow us to develop
a new EIA for serodiagnosis of Lyme disease (isoEIAplex-Ld) and to further an ongoing collaboration
with DCN diagnostics for the adaptation of our biomarkers to a new rapid Lateral Flow Assay (see
Letter of Support) for a follow up Phase II SBIR .
摘要
项目成果
期刊论文数量(9)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Fatal Multisystem Inflammatory Syndrome in Adult after SARS-CoV-2 Natural Infection and COVID-19 Vaccination.
- DOI:10.3201/eid2711.211612
- 发表时间:2021-11
- 期刊:
- 影响因子:11.8
- 作者:Grome HN;Threlkeld M;Threlkeld S;Newman C;Martines RB;Reagan-Steiner S;Whitt MA;Gomes-Solecki M;Nair N;Fill MM;Jones TF;Schaffner W;Dunn J
- 通讯作者:Dunn J
A portable immunosensor provides sensitive and rapid detection of Borrelia burgdorferi antigen in spiked blood.
- DOI:10.1038/s41598-023-34108-9
- 发表时间:2023-05-09
- 期刊:
- 影响因子:4.6
- 作者:
- 通讯作者:
A parainfluenza virus 5 (PIV5)-vectored intranasal vaccine for Lyme disease provides long-lasting protection against tick transmitted Borrelia burgdorferi in mice.
副流感病毒 5 (PIV5) 载体鼻内莱姆病疫苗可为小鼠提供针对蜱传播的伯氏疏螺旋体的持久保护。
- DOI:10.21203/rs.3.rs-3143132/v1
- 发表时间:2023
- 期刊:
- 影响因子:0
- 作者:Gingerich,MariaCristina;Nair,Nisha;Azevedo,JFilipe;Samanta,Kamalika;Kundu,Suman;He,Biao;Gomes-Solecki,Maria
- 通讯作者:Gomes-Solecki,Maria
Transient Presence of Live Leptospira interrogans in Murine Testes.
- DOI:10.1128/spectrum.02775-21
- 发表时间:2022-06-29
- 期刊:
- 影响因子:3.7
- 作者:Shetty, Advait;Kundu, Suman;Vernel-Pauillac, Frederique;Ratet, Gwendoline;Werts, Catherine;Gomes-Solecki, Maria
- 通讯作者:Gomes-Solecki, Maria
Enzyme immunoassays (EIA) for serodiagnosis of human leptospirosis: specific IgG3/IgG1 isotyping may further inform diagnosis of acute disease.
人钩端螺旋体病的酶免疫测定(EIA):特定的IgG3/IgG1同种型可能会进一步为急性疾病的诊断提供信息。
- DOI:10.1371/journal.pntd.0010241
- 发表时间:2022-03
- 期刊:
- 影响因子:3.8
- 作者:Fortes-Gabriel E;Guedes MS;Shetty A;Gomes CK;Carreira T;Vieira ML;Esteves L;Mota-Vieira L;Gomes-Solecki M
- 通讯作者:Gomes-Solecki M
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Maria Gomes-Solecki其他文献
Maria Gomes-Solecki的其他文献
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{{ truncateString('Maria Gomes-Solecki', 18)}}的其他基金
ImmunoPET Probes for the Imaging of Lyme Disease
用于莱姆病成像的免疫PET探针
- 批准号:
10802275 - 财政年份:2023
- 资助金额:
$ 29.55万 - 项目类别:
Antibody isotyping for discrimination of disease stage and diagnosis of early Lyme disease.
用于区分疾病阶段和诊断早期莱姆病的抗体同种型。
- 批准号:
10080461 - 财政年份:2020
- 资助金额:
$ 29.55万 - 项目类别:
Field trial and modeling of transmission blocking vaccine to prevent Lyme disease
预防莱姆病的传播阻断疫苗的现场试验和建模
- 批准号:
10159849 - 财政年份:2019
- 资助金额:
$ 29.55万 - 项目类别:
Field trial and modeling of transmission blocking vaccine to prevent Lyme disease
预防莱姆病的传播阻断疫苗的现场试验和建模
- 批准号:
9815231 - 财政年份:2019
- 资助金额:
$ 29.55万 - 项目类别:
Field trial and modeling of transmission blocking vaccine to prevent Lyme disease
预防莱姆病的传播阻断疫苗的现场试验和建模
- 批准号:
10636945 - 财政年份:2019
- 资助金额:
$ 29.55万 - 项目类别:
Field trial and modeling of transmission blocking vaccine to prevent Lyme disease
预防莱姆病的传播阻断疫苗的现场试验和建模
- 批准号:
10415156 - 财政年份:2019
- 资助金额:
$ 29.55万 - 项目类别:
Lab on a chip point of care assay for the rapid serodiagnosis of Lyme disease
用于莱姆病快速血清诊断的芯片即时检测实验室
- 批准号:
9052111 - 财政年份:2011
- 资助金额:
$ 29.55万 - 项目类别:
Lab on a chip point of care assay for the rapid serodiagnosis of Lyme disease
用于莱姆病快速血清诊断的芯片即时检测实验室
- 批准号:
8195733 - 财政年份:2011
- 资助金额:
$ 29.55万 - 项目类别:
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