Core Facility for Aged Rodents

摘要 老年啮齿动物核心设施(CFAR)的主要目标是开发能够 用于评估控制衰老速度并将衰老与晚年联系在一起的生理和细胞因素 疾病。新的小鼠模型经常被其他科学家提出，在密歇根大学或其他机构，这些 被评估为潜在的合作。CFAR资源用于初始表征，通常 包括对寿命、对年龄敏感的健康指数和病理学的研究，以期产生 足够的数据使新的鼠标模型可以用于竞争应用程序，以获得外部支持。在……里面 与UM OAIC的总体主题保持一致，许多开发的鼠标系统依赖于更改 代谢和/或炎症途径，无论是通过基因变化、饮食干预，还是更多 最近，毒品问题。每年选择的特定突变体、饮食或药物取决于科学发现和 前一年的互动情况。具体目标：目标1将支持#年的6个外部资助项目 第1年，包括5-羟色胺受体突变体、药物对基因表达模式的影响、抗 对癌细胞的老化药物，对特定mRNAs的帽非依赖性翻译，脂肪组织的研究 炎症，以及一种保护线粒体免受氧化的酶的过度表达分析 损坏。AIM 2使用CFAR资源来维护对小鼠衰老特别感兴趣的繁殖群体 研究包括生长激素受体组织特异性改变的突变体，IGF1结合 蛋白水解酶Pappa、线粒体氧化还原保护酶硫氧还蛋白还原酶2和两种蛋白质 促进了mRNA亚群的帽子非依赖性翻译。目标3侧重于培训、咨询和 专业教育，以便CFAR能够为PES提供关于老鼠老化模型的复杂视角 和REC应用，以及密歇根大学的学生、研究员和教职员工，并为国家 生物老年学中最佳使用啮齿动物的标准。目标4涉及选择一到两个新的创新产品 老鼠模型在每一年的发展中获奖。CFAR自1989年成立以来，提供了 地方和国家在开发新的老鼠模型方面发挥领导作用，以研究防治鼠疫 老龄化及其与晚年疾病的联系，并希望在下一个获奖期保持这一角色。

Abstract The principal goal of the Core Facility for Aged Rodents (CFAR) is to develop new mouse models that can be used to evaluate the physiological and cellular factors that control the rate of aging and tie aging to late-life diseases. New mouse models are often suggested by other scientists, at UM or at other institutions, and these are evaluated as potential collaborations. CFAR resources are used for initial characterizations, often including studies of lifespan, age-sensitive indices of health, and pathology, with a view towards producing sufficient data to allow the new mouse models to be used in competitive applications for extramural support. In keeping with the overall theme of the UM OAIC, many of the mouse systems developed depend on alterations of metabolic and/or inflammatory pathways, whether by genetic changes, dietary interventions, or, more recently, drugs. The specific mutants, diets, or drugs chosen each year depend on scientific discoveries and interactions in each preceding year. Specific Aims: Aim 1 will support six externally funded projects (EPs) in year 1, including work on serotonin receptor mutants, drug effects on gene expression patterns, effects of anti- aging drugs on cancer cells, work on cap-independent translation of specific mRNAs, studies of adipose tissue inflammation, and an analysis of over-expression of an enzyme that protect mitochondria from oxidative damage. Aim 2 uses CFAR resources to maintain breeding colonies of special interest to mouse aging research, including mutants with tissue-specific alterations in Growth Hormone receptor, the IGF1 binding protein protease PAPPA, the mitochondrial redox protecting enzyme thioredoxin reductase 2, and two proteins that promote cap-independent translation of mRNA subsets. Aim 3 focuses on training, consultation, and professional education, so that CFAR can provide sophisticated perspectives on mouse aging models to PES and REC applications, and to UM students, fellows and faculty, and contribute to development of national standards for optimal use of rodents in biogerontology. Aim 4 involves selection of one or two new innovative mouse models for development in each year of the award. CFAR has, since its inception in 1989, provided both local and national leadership in the development of new mouse models for research on the control of aging and its links to late-life diseases, and hopes to maintain this role in the next award period.