Expansion of the ‘Getting Started in Cryo-EM’ course into a comprehensive theory and practice curriculum

将“Cryo-EM 入门”课程扩展为综合理论和实践课程

• 批准号: 10223807
• 负责人: GRANT J JENSEN
• 金额: $ 12.42万
• 依托单位: CALIFORNIA INSTITUTE OF TECHNOLOGY
• 依托单位国家: 美国
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-06-01 至 2024-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10223807
• 关键词: 3-Dimensional; Biological; Cells; Cellular Structures; Communities; Competence; Cryoelectron Microscopy; Crystallization; Data; Development; Disease; Drug Design; Education; Educational Curriculum; Educational process of instructing; Educational workshop; Electron Microscopy; Electrons; Elements; Emerging Technologies; Enrollment; Equipment; Evaluation; Exercise; Feedback; Freezing; Goals; Home; Hour; Hydration status; Image; Individual; Institution; Ions; Knowledge; Learning; Licensing; Manuals; Microscope; Modality; Preparation; Program Development; Proteins; Research Personnel; Resolution; Resources; Safety; Sampling; Schools; Services; Standardization; Structural Biologist; Structural Protein; Structure; Students; Supervision; System; Techniques; Technology; Training; Training Programs; Update; Virus; Water; Work; career; combat; cryogenics; curriculum development; design; electron diffraction; experience; graduate student; image processing; instrument; lectures; light microscopy; macromolecule; massive open online courses; online course; operation; particle; preservation; programs; protein complex; reconstruction; response; skills; structural biology; submicron; theories

Project Summary Cryogenic electron microscopy (cryo-EM) is a rapidly emerging technology for structural biology. While excitement and demand for the technology are high, the pace of training cryo-EM practitioners lags far behind. There are two major hurdles to the development of effective training programs: (1) potential learners come from a wide range of backgrounds and career stages, from graduate students to established structural biologists; and (2) learning cryo-EM requires many hours of hands-on “behind the wheel” training on specialized, expensive equipment that is often unavailable to learners at their home institutions. While some hands-on schools exist for cryoEM, they train only a handful of students a year, while demand for the technique requires orders of magnitude more. Workshops enroll more students, but are too short to teach all the necessary theory and lack a hands-on component. To train a skilled workforce of cryo-EM practitioners, here I propose to expand upon my successful “Getting Started in Cryo-EM” video lectures and create a comprehensive online curriculum covering the theory and practice of all major cryo-EM modalities including cryo-EM single particle reconstruction (SPR), electron cryotomography (cryo-ET) and microcrystal electron diffraction (micro-ED). This curriculum will include many tens of hours of new videos and also the supporting materials needed to maximize the usefulness of these videos. More specifically, my colleagues and I will produce new videos teaching the theory and practice of cryo-ET (including special sample prep issues like correlated light and electron microscopy and cryo-FIB milling), micro-ED, and facility management/advanced topics. We will also produce videos that introduce basic principles of cryo-EM image processing and prepare students to move from our materials to the specialized tutorials already available for several popular image processing packages. We will also create supporting materials including homework problems, open-ended and multiple-choice exam questions, training manuals with specific standardized practice exercises, and plans for training programs. Together with staff from the national cryo-EM service centers and my fellow curriculum development program awardees, I will work to develop a “merit badge” system to certify cryo-EM practitioners on specific skills and instruments. Materials will be designed in modules that can be mixed and matched to meet the specific needs of the learner. In conjunction with supervised training at a user’s home facility or national cryo-EM service center, the curriculum will offer everything a student needs to understand the theory of, and begin practicing, their desired cryo-EM technique.

项目摘要 低温电子显微镜（Cryo-EM）是一种用于结构生物学的快速新兴技术。尽管 对技术的兴奋和需求很高，培训冷冻EM从业者的速度远远落后。 开发有效培训计划有两个主要障碍：（1）潜在的学习者来了 从各种背景和职业阶段，从研究生到建立的结构 生物学家； （2）学习Cryo-EM需要进行许多小时的动手“在方向盘后”训练 专门的，昂贵的设备通常是他们家庭机构的学习者无法获得的。而有些 动手学校存在于冷冻的情况下，他们每年只培训少数学生，而对 技术需要更多的数量级。研讨会注册了更多的学生，但太短了，无法教所有学生 必要的理论，缺乏动手组成部分。要培训熟练的冷冻EM从业者的劳动力， 在这里，我建议扩展我成功的“入门在Cryo-Em”视频讲座上，并创建一个 全面的在线课程涵盖了所有主要的低温EM方式的理论和实践 冷冻 - EM单粒子重建（SPR），电子冷冻理学（冷冻-ET）和微晶电子 衍射（微型ED）。该课程将包括数十个小时的新视频和支持 最大化这些视频的实用性所需的材料。更具体地说，我的同事和我会 制作新视频教授Cryo-ET的理论和实践（包括特殊的样本准备问题，例如 相关的光和电子显微镜以及冷冻纤维铣削），微观和设施管理/高级 主题。我们还将制作介绍Cryo-Em图像处理的基本原理并准备的视频 学生可以从我们的材料转移到已经可用于几张流行图像的专业教程 处理软件包。我们还将创建支持材料，包括家庭作业问题，开放式和 多项选择考试问题，具有特定标准化练习的培训手册以及计划 培训计划。与国家低温EM服务中心的工作人员和我的其他课程 开发计划获奖者，我将努力开发一个“功绩徽章”系统，以证明冷冻EM从业者 关于特定技能和工具。材料将以可以混合并匹配的模块设计 满足学习者的具体需求。结合用户家庭设施的监督培训或 国家冷冻EM服务中心，该课程将为学生提供理论所需的一切 并开始练习，是他们所需的冷冻EM技术。