Cell Immunology and Metabolism Core (CIMC)

摘要 路易斯安那州立大学癌症中心的细胞免疫学和代谢核心（CIMC）提供尖端的流动 包括COBRE PJI在内的LSUHSC研究人员，以及 我们地区的其他用户。CIMC实验室现在提供全面的分析流式细胞术，高 加速细胞分选服务，功能性检测，包括抗原特异性和有丝分裂原诱导的细胞增殖， 细胞因子产生、细胞死亡和存活、细胞介导的细胞毒性和效应T细胞频率。中集 我还获得并发展了研究细胞代谢（包括氧气）的基础设施和专业知识 消耗和酸化率，线粒体功能;以及通过检测免疫介质 多元方法因此，中集集团最先进的服务非常适合支持 新一期COBRE申请“转化病毒肿瘤学中心”（CTVO）的PJIs需求。这 将通过充分利用现有的服务和资源，并通过扩大其专门知识，以确定新的 用于病毒诱导的癌症的生物标志物，与病毒诱导的癌症相关的细胞生长和细胞死亡应答的评价， 病毒癌蛋白的表达和抗癌治疗。此外，创新的方法， 由中集集团人员持续实施，将使PJIs及其导师能够开发新的 实验策略解释病毒诱导的肿瘤细胞信号水平的变化，表观遗传 调节和肿瘤细胞的能量代谢，从而增加他们的机会，高影响力的出版物， 联邦和基金会相关的资金。这些先进的技术使中集集团成为领先的 该区域的癌症和免疫学实验室，这导致用户人数增加， 包括CTVO的PJIs，以及它作为LSUHSC医学院和 路易斯安那州立大学癌症中心。 ! !

ABSTRACT The Cellular Immunology and Metabolism Core (CIMC) of the LSU Cancer Center provides cutting edge flow cytometry, immunology and metabolism technologies LSUHSC investigators including COBRE PJI's, and to other users in our region. The CIMC laboratory now provides comprehensive analytic flow cytometry, high- speed cell sorting services, functional assays including antigen-specific and mitogen-induced cell proliferation, cytokine production, cell death and survival, cell mediated cytotoxicity, and effector T cell frequency. The CIMC also acquired and developed the infrastructure and expertise to study cellular metabolism, including oxygen consumption and acidification rates, mitochondrial function; and the detection of immune mediators through multiplex approaches. Therefore, state-of-the-art services of the CIMC are very well suited to support the needs of PJIs of the new Phase I COBRE application ”Center for translational Viral Oncology” (CTVO). This will be done by fully utilizing present services and resources, and by expending their expertise to identify new biomarkers for virus-induced cancer, evaluation of cell growth and cell death responses associated with the expression of viral oncoproteins and anticancer treatment. In addition, innovative approaches, which are continuously implemented by the CIMC personnel will allow PJIs and their mentors to develop new experimental strategies explaining virus-induced changes at the levels of tumor cell signaling, epigenetic regulation and tumor cell energy metabolism, thereby increasing their chances for high impact publications and federal and foundation-related funding. These advanced technologies have positioned the CIMC as the leading laboratory in cancer and immunology in the region, which has resulted in an increased number of users, including PJIs of the CTVO, and its integration as a core laboratory at the LSUHSC School of Medicine and the LSU-Cancer Center. ! !