Citizen DNA Barcode Network: A Community-based Infrastructure for Monitoring Biodiversity and Disease Vectors

与纽约科学馆(NYSCI)合作，DNA学习中心 冷泉港实验室(DNALC)建议开发公民DNA 条形码网络。该项目将组织全国性的运动，绘制出 昆虫分为三类：蚂蚁、蚊子和甲虫。这些团体 包括环境变化生物指标、人类疾病的病媒以及 经济上重要的物种，其分布范围正因全球气候变化而改变。 公民科学家将使用DNA条形码技术，这允许非专家 识别几乎所有的昆虫物种，即使是从幼虫形态或损坏的样本。 该项目基于我们已有的综合生化和生物信息学工作流程 开发用于在正规教育环境中支持DNA条形码。我们目前的环保总局 Barcode Long Island项目已经在正式的 教育系统--让高中生记录当地的生物多样性和 几种昆虫的活动范围向北扩展--全球变暖的新证据。 在开发阶段，我们将与NYSCI密切合作，完善资源 以及在非正式环境中支持公民科学的传播方法。同时，我们 将试行吸引游客参观自然保护区和自然保护成员的方法 团体，包括自然保护协会和奥杜邦协会。在复制期间 阶段，我们将增加三个科学中心和六个社区 保护组织。将为合作伙伴提供培训、教育材料、移动电话 为昆虫运动和生物多样性调查生成数据所需的实验室和用品 (生物群落)当地重要的栖息地。由高中和大学教职员工提供的指导 在DNA条形码和扩展协作支持方面的经验-包括 故障排除和数据分析-将有助于确保每个站点的成功。 公民科学家收集的数据将以两种方式为科学记录做出贡献：1) 条形码序列将在权威DNA数据库GenBank中发布，并将 物种特定数据库。2)事件数据将贡献给全局 生物多样性信息设施(GBIF)，以扩展现有的范围图。一个多方面的 评估计划将开发新的调查工具，以评估 参与公民对全球生物多样性态度的条形码项目 气候变化。

In collaboration with the New York Hall of Science (NYSCI), the DNA Learning Center (DNALC) of Cold Spring Harbor Laboratory (CSHL) proposes to develop a Citizen DNA Barcode Network. The project will organize national campaigns to map the ranges of species within three groups of insect: ants, mosquitoes, and beetles. These groups include bioindicators of environmental change, vectors of human disease, and economically important species whose ranges are being altered by global climate change. Citizen scientists will use the technique of DNA barcoding, which allows non-experts to identify almost any insect species, even from larval forms or damaged samples. The project is based on an integrated biochemical and bioinformatics workflow we have developed to support DNA barcoding in formal education settings. Our current SEPA project, Barcode Long Island, has undertaken DNA barcoding campaigns in the formal education system—with high school students documenting local biodiversity and the northward range expansion of several insects—novel evidence of global warming. During the development phase, we will work intensively with NYSCI to refine resources and dissemination methods to support citizen science in informal settings. In parallel, we will pilot methods to engage visitors to nature preserves and members of conservation groups, including The Nature Conservancy and Audubon Society. During the replication phase, we will onboard three additional science centers and six additional community conservation groups. Partners will be provided with training, educational materials, mobile labs, and supplies needed to generate data for insect campaigns and biodiversity surveys (bioblitzes) of locally important habitats. Mentorship by high school and college faculty with experience in DNA barcoding and extended collaborative support—including troubleshooting and data analysis—will help insure success at each site. Data collected by citizen scientists will contribute to the scientific record in two ways: 1) Barcode sequences will be published in GenBank, the authoritative DNA database, and to species-specific databases. 2) Occurrence data will be contributed to the Global Biodiversity Information Facility (GBIF) to expand existing range maps. A multi-faceted evaluation program will develop novel survey instruments to assess the impact of participation in citizen barcoding projects on attitudes toward biodiversity on global climate change.