Citizen DNA Barcode Network: A Community-based Infrastructure for Monitoring Biodiversity and Disease Vectors

公民 DNA 条形码网络：用于监测生物多样性和疾病媒介的社区基础设施

• 批准号: 10397687
• 负责人: David Andrew MICKLOS
• 金额: $ 26.42万
• 依托单位: COLD SPRING HARBOR LABORATORY
• 依托单位国家: 美国
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-06-01 至 2025-03-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10397687
• 关键词: Ants; Arbovirus Infections; Attitude; Bar Codes; Biochemical; Biodiversity; Bioinformatics; Collaborations; Communities; County; Culicidae; DNA; DNA Databases; Data; Data Analyses; Databases; Development; Disease Vectors; Education; Education and Outreach; Educational Materials; Equipment; Equipment and supply inventories; Evaluation; Faculty; Genbank; Global Warming; Goals; Habitats; Health; High School Student; Infrastructure; Insecta; Laboratories; Learning; Long Island; Maps; Mentorship; Methods; Modeling; Monitor; Museums; Nature; New York; Order Coleoptera; Organism; Participant; Phase; Program Evaluation; Publishing; Research; Resources; Sampling; Science; Scientist; Site; Societies; Structure; Surveys; System; Taxonomy; Teacher Professional Development; Techniques; Training; Work; base; citizen science; climate change; college; design; environmental change; experience; high school; human disease; improved; interest; member; novel; outreach; outreach program; preservation; programs; science education; science museum; success; symposium; vector; web interface

In collaboration with the New York Hall of Science (NYSCI), the DNA Learning Center (DNALC) of Cold Spring Harbor Laboratory (CSHL) proposes to develop a Citizen DNA Barcode Network. The project will organize national campaigns to map the ranges of species within three groups of insect: ants, mosquitoes, and beetles. These groups include bioindicators of environmental change, vectors of human disease, and economically important species whose ranges are being altered by global climate change. Citizen scientists will use the technique of DNA barcoding, which allows non-experts to identify almost any insect species, even from larval forms or damaged samples. The project is based on an integrated biochemical and bioinformatics workflow we have developed to support DNA barcoding in formal education settings. Our current SEPA project, Barcode Long Island, has undertaken DNA barcoding campaigns in the formal education system—with high school students documenting local biodiversity and the northward range expansion of several insects—novel evidence of global warming. During the development phase, we will work intensively with NYSCI to refine resources and dissemination methods to support citizen science in informal settings. In parallel, we will pilot methods to engage visitors to nature preserves and members of conservation groups, including The Nature Conservancy and Audubon Society. During the replication phase, we will onboard three additional science centers and six additional community conservation groups. Partners will be provided with training, educational materials, mobile labs, and supplies needed to generate data for insect campaigns and biodiversity surveys (bioblitzes) of locally important habitats. Mentorship by high school and college faculty with experience in DNA barcoding and extended collaborative support—including troubleshooting and data analysis—will help insure success at each site. Data collected by citizen scientists will contribute to the scientific record in two ways: 1) Barcode sequences will be published in GenBank, the authoritative DNA database, and to species-specific databases. 2) Occurrence data will be contributed to the Global Biodiversity Information Facility (GBIF) to expand existing range maps. A multi-faceted evaluation program will develop novel survey instruments to assess the impact of participation in citizen barcoding projects on attitudes toward biodiversity on global climate change.

与纽约科学馆（NYSCI）合作，DNA学习中心 冷泉港实验室（CSHL）的DNALC提出开发一种公民DNA 条码网络。该项目将组织全国运动， 三种昆虫：蚂蚁、蚊子和甲虫。这些群体 包括环境变化生物指示剂、人类疾病的媒介， 其分布范围正因全球气候变化而改变的重要经济物种。 公民科学家将使用DNA条形码技术，这使得非专家， 鉴定几乎所有昆虫种类，甚至从幼虫形态或受损样本。 该项目是基于一个综合的生物化学和生物信息学工作流程，我们有 开发用于支持正规教育环境中的DNA条形码。我们目前的SEPA Barcode Long Island项目已经在正式的DNA条形码项目中开展了DNA条形码活动。 教育系统，高中学生记录当地的生物多样性和 几种昆虫向北的活动范围扩张--全球变暖的新证据。 在开发阶段，我们将与NYSCI密切合作， 和传播方法，以支持非正式环境中的公民科学。同时，我们 将试行一些方法，让游客和自然保护区的成员参与进来， 团体，包括大自然保护协会和奥杜邦协会。在复制期间 在此阶段，我们将增加三个科学中心和六个社区 保护团体。将为合作伙伴提供培训、教育材料、移动的 实验室，以及为昆虫活动和生物多样性调查生成数据所需的用品 （生物块）当地重要的栖息地。高中和大学教师的指导， 在DNA条形码和扩展合作支持方面的经验，包括 故障排除和数据分析-将有助于确保每个站点的成功。 公民科学家收集的数据将以两种方式为科学记录做出贡献：1） 条形码序列将在权威的DNA数据库GenBank中公布， 物种特异性数据库。2)发生率数据将提供给全球 生物多样性信息设施（GBIF），以扩大现有的范围地图。多方面 评估计划将开发新的调查工具，以评估 参与公民对全球生物多样性态度的条形码项目 气候变化