Engineered salivary gland tissue chips (Administrative Supplement)
工程唾液腺组织芯片(行政补充)
基本信息
- 批准号:10426429
- 负责人:
- 金额:$ 4.38万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2017
- 资助国家:美国
- 起止时间:2017-08-01 至 2024-02-29
- 项目状态:已结题
- 来源:
- 关键词:Administrative SupplementBiological AssayBiologyCalcium SignalingCommunicationCuesDataData SetDatabase Management SystemsDatabasesDevelopmentDevicesDisease modelDrug ScreeningEngineeringGene ExpressionGoalsHumanImmunohistochemistryLibrariesLipid PeroxidationLongevityMetabolicModelingMusPharmaceutical PreparationsPhenotypeProtocols documentationPublicationsRadiation ProtectionRadiation induced damageReactive Oxygen SpeciesReproducibilityRoleSalivary Gland TissueSalivary GlandsTissue MicroarrayTissue ModelTissuesbasedata submissiondrug candidateexperimental studyhuman tissueimprovedin vivomicrophysiology systemmimeticsnovelnovel therapeuticsparent grantsaliva secretion
项目摘要
ABSTRACT
The goal of this supplement is to establish a workflow to upload data generated from our developed salivary
gland tissue chip to the microphysiological (MPS) database (MPS-DB). Our data represents a new tissue
model that is not currently represented in the MPS-DB. In addition, our model uses a novel device and includes
a new disease model: salivary gland radiation damage.
Overall, this supplement has 3 major goals:
· Establish a workflow to upload data to the microphysiological systems (MPS) database (MPS-DB).
Data for deposition are from past experiments focused on developing and validating the tissue chip, as
well as ongoing drug screening experiments to verify the role of known radioprotective, sialogenic, and
xerogenic drugs and screen for new drug candidates.
· Upload our current protocols and model, including detailed standard operating practices and assays
used to characterize and validate the tissue chips.
· Upload our current data, which includes data to support reproducibility and relevance to phenotype and
function of naïve salivary glands for both mouse and human tissue.
Data generated by the parent grant includes/will include the following:
· Salivary gland tissue mimetic
o Viability
o metabolic activity
o gene expression
o immunohistochemistry
o calcium signaling
o reactive oxygen species quantification
o lipid peroxidation
· Saliva secretion (in vivo)
These data pertain to chip development, which shows reproducibility and relevance to human and mouse
salivary gland phenotype and function. These data are detailed in Song et al., Communications Biology, 2021
and two forthcoming publications, which detail matrix and soluble cues to improve the longevity of tissue
mimetic function. We have ongoing experiments focused on known and library-based drug screening for
radioprotective, sialogenic, and xerogenic drugs. Hence, our current data set submission will enable workflow
development alongside ongoing experiments, streamlining the deposition of data.
摘要
本补充的目标是建立一个工作流程来上传从我们开发的唾液中生成的数据
腺组织芯片的微生理(MPS)数据库(MPS-DB)。我们的数据代表了一种新的组织
目前在MPS-DB中没有表示的模型。此外,我们的模型使用了一种新颖的设备,
一种新的疾病模型:唾液腺辐射损伤。
总的来说,这个补充有三个主要目标:
·建立将数据上传到微生理系统(MPS)数据库(MPS-DB)的工作流程。
用于沉积的数据来自过去的实验,这些实验集中于开发和验证组织芯片,
以及正在进行的药物筛选实验,以验证已知的辐射保护,唾液腺,
干燥药物和筛选新的候选药物。
·上传我们当前的协议和模型,包括详细的标准操作规范和分析
用于表征和验证组织芯片。
·上传我们当前的数据,其中包括支持再现性和与表型相关性的数据,
小鼠和人体组织的幼稚唾液腺功能。
父母补助金产生的数据包括/将包括以下内容:
·唾液腺组织模拟物
o生存能力
o代谢活性
o基因表达
o免疫组织化学
o钙信号传导
o活性氧定量
o脂质过氧化
·唾液分泌(体内)
这些数据属于芯片开发,显示了对人类和小鼠的可重复性和相关性
唾液腺表型和功能。这些数据在Song等人中有详细描述,通信生物学,2021
和两个即将出版的出版物,其中详细介绍了基质和可溶性线索,以提高组织的寿命
模仿功能我们正在进行的实验集中在已知的和基于库的药物筛选,
辐射防护、唾液生成和致干药物。因此,我们当前的数据集提交将启用工作流
开发与正在进行的实验,简化数据的沉积。
项目成果
期刊论文数量(1)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Long-Term Maintenance of Acinar Cells in Human Submandibular Glands After Radiation Therapy.
- DOI:10.1016/j.ijrobp.2020.10.037
- 发表时间:2021-03-15
- 期刊:
- 影响因子:0
- 作者:Luitje ME;Israel AK;Cummings MA;Giampoli EJ;Allen PD;Newlands SD;Ovitt CE
- 通讯作者:Ovitt CE
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
数据更新时间:{{ journalArticles.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ monograph.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ sciAawards.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ conferencePapers.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ patent.updateTime }}
Danielle S. Benoit其他文献
Danielle S. Benoit的其他文献
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
{{ truncateString('Danielle S. Benoit', 18)}}的其他基金
Tissue Engineering Strategies to Revitalize Allografts
振兴同种异体移植物的组织工程策略
- 批准号:
10830613 - 财政年份:2023
- 资助金额:
$ 4.38万 - 项目类别:
Using hiPSCs to develop physiologically-relevant outer retina tissue mimetics
使用 hiPSC 开发生理相关的外视网膜组织模拟物
- 批准号:
10467753 - 财政年份:2022
- 资助金额:
$ 4.38万 - 项目类别:
Tendon TRAP: Targeted Therapeutic Delivery to Enhance Tendon Healing
Tendon TRAP:有针对性的治疗交付以增强肌腱愈合
- 批准号:
10461486 - 财政年份:2022
- 资助金额:
$ 4.38万 - 项目类别:
Bone-targeted polymer therapeutics for non-union fracture healing
用于骨不连骨折愈合的骨靶向聚合物治疗
- 批准号:
10681217 - 财政年份:2022
- 资助金额:
$ 4.38万 - 项目类别:
Tendon TRAP: Targeted Therapeutic Delivery to Enhance Tendon Healing
Tendon TRAP:有针对性的治疗交付以增强肌腱愈合
- 批准号:
10612076 - 财政年份:2022
- 资助金额:
$ 4.38万 - 项目类别:
Bone-targeted polymer therapeutics for nonunion fracture healing
用于骨不连骨折愈合的骨靶向聚合物治疗
- 批准号:
10371267 - 财政年份:2022
- 资助金额:
$ 4.38万 - 项目类别:
Bone-targeted polymer therapeutics for non-union fracture healing
用于骨不连骨折愈合的骨靶向聚合物治疗
- 批准号:
10733942 - 财政年份:2022
- 资助金额:
$ 4.38万 - 项目类别:
Using hiPSCs to develop physiologically-relevant outer retina tissue mimetics
使用 hiPSC 开发生理相关的外视网膜组织模拟物
- 批准号:
10709483 - 财政年份:2022
- 资助金额:
$ 4.38万 - 项目类别:
hiPSC-derived tissue mimetics of the retina blood barrier
hiPSC 衍生的视网膜血屏障组织模拟物
- 批准号:
10080730 - 财政年份:2020
- 资助金额:
$ 4.38万 - 项目类别:
相似海外基金
Establishment of a new biological assay using Hydra nematocyst deployment
利用水螅刺丝囊部署建立新的生物测定方法
- 批准号:
520728-2017 - 财政年份:2017
- 资助金额:
$ 4.38万 - 项目类别:
University Undergraduate Student Research Awards
POINT-OF-CARE BIOLOGICAL ASSAY FOR DETERMINING TISSUE-SPECIFIC ABSORBED IONIZING RADIATION DOSE (BIODOSIMETER) AFTER RADIOLOGICAL AND NUCLEAR EVENTS.
用于确定放射和核事件后组织特异性吸收电离辐射剂量(生物剂量计)的护理点生物测定。
- 批准号:
10368760 - 财政年份:2017
- 资助金额:
$ 4.38万 - 项目类别:
POINT-OF-CARE BIOLOGICAL ASSAY FOR DETERMINING TISSUE-SPECIFIC ABSORBED IONIZING RADIATION DOSE (BIODOSIMETER) AFTER RADIOLOGICAL AND NUCLEAR EVENTS.
用于确定放射和核事件后组织特异性吸收电离辐射剂量(生物剂量计)的护理点生物测定。
- 批准号:
10669539 - 财政年份:2017
- 资助金额:
$ 4.38万 - 项目类别:
POINT-OF-CARE BIOLOGICAL ASSAY FOR DETERMINING TISSUE-SPECIFIC ABSORBED IONIZING RADIATION DOSE (BIODOSIMETER) AFTER RADIOLOGICAL AND NUCLEAR EVENTS.
用于确定放射和核事件后组织特异性吸收电离辐射剂量(生物剂量计)的护理点生物测定。
- 批准号:
9570142 - 财政年份:2017
- 资助金额:
$ 4.38万 - 项目类别:
POINT-OF-CARE BIOLOGICAL ASSAY FOR DETERMINING TISSUE-SPECIFIC ABSORBED IONIZING RADIATION DOSE (BIODOSIMETER) AFTER RADIOLOGICAL AND NUCLEAR EVENTS.
用于确定放射和核事件后组织特异性吸收电离辐射剂量(生物剂量计)的护理点生物测定。
- 批准号:
9915803 - 财政年份:2017
- 资助金额:
$ 4.38万 - 项目类别:
COVID-19 Supplemental work: POINT-OF-CARE BIOLOGICAL ASSAY FOR DETERMINING TISSUE-SPECIFIC ABSORBED IONIZING RADIATION DOSE (BIODOSIMETER).
COVID-19 补充工作:用于确定组织特异性吸收电离辐射剂量的护理点生物测定(生物剂量计)。
- 批准号:
10259999 - 财政年份:2017
- 资助金额:
$ 4.38万 - 项目类别:
Drug discovery based on a new biological assay system using Yeast knock-out strain collection
基于使用酵母敲除菌株收集的新生物测定系统的药物发现
- 批准号:
21580130 - 财政年份:2009
- 资助金额:
$ 4.38万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Machine learning for automatic gene annotation using high-throughput biological assay data
使用高通量生物测定数据进行自动基因注释的机器学习
- 批准号:
300985-2004 - 财政年份:2005
- 资助金额:
$ 4.38万 - 项目类别:
Postdoctoral Fellowships
Machine learning for automatic gene annotation using high-throughput biological assay data
使用高通量生物测定数据进行自动基因注释的机器学习
- 批准号:
300985-2004 - 财政年份:2004
- 资助金额:
$ 4.38万 - 项目类别:
Postdoctoral Fellowships