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Engineered salivary gland tissue chips (Administrative Supplement)

工程唾液腺组织芯片（行政补充）

基本信息

批准号：
10426429
负责人：
Danielle S. Benoit
金额：
$ 4.38万
依托单位：
UNIVERSITY OF ROCHESTER
依托单位国家：
美国
项目类别：
财政年份：
2017
资助国家：
美国
起止时间：
2017-08-01 至 2024-02-29
项目状态：
已结题

项目摘要

ABSTRACT The goal of this supplement is to establish a workflow to upload data generated from our developed salivary gland tissue chip to the microphysiological (MPS) database (MPS-DB). Our data represents a new tissue model that is not currently represented in the MPS-DB. In addition, our model uses a novel device and includes a new disease model: salivary gland radiation damage. Overall, this supplement has 3 major goals: · Establish a workflow to upload data to the microphysiological systems (MPS) database (MPS-DB). Data for deposition are from past experiments focused on developing and validating the tissue chip, as well as ongoing drug screening experiments to verify the role of known radioprotective, sialogenic, and xerogenic drugs and screen for new drug candidates. · Upload our current protocols and model, including detailed standard operating practices and assays used to characterize and validate the tissue chips. · Upload our current data, which includes data to support reproducibility and relevance to phenotype and function of naïve salivary glands for both mouse and human tissue. Data generated by the parent grant includes/will include the following: · Salivary gland tissue mimetic o Viability o metabolic activity o gene expression o immunohistochemistry o calcium signaling o reactive oxygen species quantification o lipid peroxidation · Saliva secretion (in vivo) These data pertain to chip development, which shows reproducibility and relevance to human and mouse salivary gland phenotype and function. These data are detailed in Song et al., Communications Biology, 2021 and two forthcoming publications, which detail matrix and soluble cues to improve the longevity of tissue mimetic function. We have ongoing experiments focused on known and library-based drug screening for radioprotective, sialogenic, and xerogenic drugs. Hence, our current data set submission will enable workflow development alongside ongoing experiments, streamlining the deposition of data.

摘要本补充的目标是建立一个工作流程来上传从我们开发的唾液中生成的数据腺组织芯片的微生理（MPS）数据库（MPS-DB）。我们的数据代表了一种新的组织目前在MPS-DB中没有表示的模型。此外，我们的模型使用了一种新颖的设备，一种新的疾病模型：唾液腺辐射损伤。总的来说，这个补充有三个主要目标： ·建立将数据上传到微生理系统（MPS）数据库（MPS-DB）的工作流程。用于沉积的数据来自过去的实验，这些实验集中于开发和验证组织芯片，以及正在进行的药物筛选实验，以验证已知的辐射保护，唾液腺，干燥药物和筛选新的候选药物。 ·上传我们当前的协议和模型，包括详细的标准操作规范和分析用于表征和验证组织芯片。 ·上传我们当前的数据，其中包括支持再现性和与表型相关性的数据，小鼠和人体组织的幼稚唾液腺功能。父母补助金产生的数据包括/将包括以下内容： ·唾液腺组织模拟物 o生存能力 o代谢活性 o基因表达 o免疫组织化学 o钙信号传导 o活性氧定量 o脂质过氧化 ·唾液分泌（体内）这些数据属于芯片开发，显示了对人类和小鼠的可重复性和相关性唾液腺表型和功能。这些数据在Song等人中有详细描述，通信生物学，2021 和两个即将出版的出版物，其中详细介绍了基质和可溶性线索，以提高组织的寿命模仿功能我们正在进行的实验集中在已知的和基于库的药物筛选，辐射防护、唾液生成和致干药物。因此，我们当前的数据集提交将启用工作流开发与正在进行的实验，简化数据的沉积。