权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

Engineered salivary gland tissue chips (Administrative Supplement)

工程唾液腺组织芯片（行政补充）

基本信息

批准号：
10426429
负责人：
Danielle S. Benoit
金额：
$ 4.38万
依托单位：
UNIVERSITY OF ROCHESTER
依托单位国家：
美国
项目类别：
财政年份：
2017
资助国家：
美国
起止时间：
2017-08-01 至 2024-02-29
项目状态：
已结题

项目摘要

ABSTRACT The goal of this supplement is to establish a workflow to upload data generated from our developed salivary gland tissue chip to the microphysiological (MPS) database (MPS-DB). Our data represents a new tissue model that is not currently represented in the MPS-DB. In addition, our model uses a novel device and includes a new disease model: salivary gland radiation damage. Overall, this supplement has 3 major goals: · Establish a workflow to upload data to the microphysiological systems (MPS) database (MPS-DB). Data for deposition are from past experiments focused on developing and validating the tissue chip, as well as ongoing drug screening experiments to verify the role of known radioprotective, sialogenic, and xerogenic drugs and screen for new drug candidates. · Upload our current protocols and model, including detailed standard operating practices and assays used to characterize and validate the tissue chips. · Upload our current data, which includes data to support reproducibility and relevance to phenotype and function of naïve salivary glands for both mouse and human tissue. Data generated by the parent grant includes/will include the following: · Salivary gland tissue mimetic o Viability o metabolic activity o gene expression o immunohistochemistry o calcium signaling o reactive oxygen species quantification o lipid peroxidation · Saliva secretion (in vivo) These data pertain to chip development, which shows reproducibility and relevance to human and mouse salivary gland phenotype and function. These data are detailed in Song et al., Communications Biology, 2021 and two forthcoming publications, which detail matrix and soluble cues to improve the longevity of tissue mimetic function. We have ongoing experiments focused on known and library-based drug screening for radioprotective, sialogenic, and xerogenic drugs. Hence, our current data set submission will enable workflow development alongside ongoing experiments, streamlining the deposition of data.

摘要本附录的目标是建立一个工作流来上传从我们开发的唾液中生成的数据微生理学(MPS)数据库(MPS-DB)的腺体组织芯片。我们的数据代表了一种新的组织 MPS-DB中当前未表示的型号。此外，我们的模型使用了一种新的设备，包括一种新的疾病模型：唾液腺辐射损伤。总体而言，本补充文件有三个主要目标： ·建立将数据上传到微生理系统(MPS-DB)数据库的工作流程。用于沉积的数据来自过去专注于开发和验证组织芯片的实验，因为以及正在进行的药物筛选实验，以验证已知的辐射防护、生唾液和干燥药物和筛选新的候选药物。 ·上传我们当前的方案和模型，包括详细的标准操作实践和分析用于表征和验证组织芯片。 ·上传我们当前的数据，其中包括支持可再生性和与表型和幼稚的唾液腺对小鼠和人类组织都有作用。父赠款生成的数据包括/将包括以下内容： ·模拟唾液腺组织 O生存能力 O代谢活动 O基因表达 O免疫组织化学 O钙信号 O活性氧物种定量化 O脂质过氧化 ·唾液分泌(体内) 这些数据与芯片开发有关，这表明了重复性和与人类和老鼠的相关性唾液腺的表型和功能。这些数据在Song等人的《通信生物学，2021》中有详细介绍以及即将出版的两份出版物，详细介绍了提高组织寿命的基质和可溶线索模仿功能。我们有正在进行的实验，重点是已知的和基于库的药物筛选防辐射、生唾液和致干燥的药物。因此，我们目前提交的数据集将启用工作流开发与正在进行的实验同时进行，简化了数据的存储。