Localized mRNA translation on the spindle -an essential mechanism for embryonic cell regulation

纺锤体上的局部 mRNA 翻译——胚胎细胞调节的重要机制

• 批准号: 10297862
• 负责人: Mamiko Yajima
• 金额: $ 34.13万
• 依托单位: BROWN UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2017
• 资助国家: 美国
• 起止时间: 2017-12-26 至 2023-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10297862
• 关键词: Animals; Binding; Biological; Biological Assay; Body Patterning; Cell Fate Control; Cell division; Cell physiology; Cells; Cellular biology; Communities; Defect; Development; Developmental Biology; Diffusion; Embryo; Embryonic Development; Event; Functional disorder; Future; Gene Proteins; Genetic Translation; Germ Lines; Imaging Techniques; Imaging technology; In Vitro; Kinetics; Lead; Light; Membrane; Messenger RNA; Methods; Microtubules; Mitosis; Mitotic; Mitotic Spindle Apparatus; Mitotic spindle; Molecular; Organism; Outcome; Process; Production; Protein Biosynthesis; Protein Dynamics; Proteins; Proteomics; RNA Helicase; Regulation; Reporting; Research; Research Personnel; Role; Sea Urchins; Side; Site; Testing; Time; Translating; Translational Regulation; Translations; base; blastomere structure; cell fate specification; cell growth regulation; cellular development; chromophore; daughter cell; detection platform; egg; embryo cell; embryo stage 2; gain of function; in vivo; in vivo imaging; insight; knock-down; loss of function; optogenetics; recruit

Project Summary/Abstract Localization of mRNAs to specific cells of an embryo, or to sub-cellular regions in the cell, enables spatially controlled protein production. Distinct concentrations of proteins lead to differential cell fate determination, body patterning, and cell function during development in many animals. Especially, this mechanism of localized translation appears essential in embryonic cells that are large and that undergo rapid cell divisions, requiring immediate input of specific protein without relying on diffusion kinetics from translation elsewhere in the cell. Indeed, the mitotic spindle is a sub-cellular region where localization of mRNAs has been reported in eggs/ embryos of various organisms. The mechanism or process of localized translation on the spindle has been, however, little identified. The proposed research focuses on a conserved DEAD-box RNA helicase Vasa that is localized on the spindle of every blastomere during embryogenesis. We previously identified Vasa functions in general translational regulation and its dysfunction results in ~80% reduction of protein synthesis and mitotic defects in the sea urchin embryo. We, therefore, hypothesize in these embryonic cells, Vasa contributes to the process of localized mRNA translation on the spindle. To test our hypothesis, using sea urchin embryos that show constant and robust Vasa expression on the spindle of every blastomere, we will visualize in vivo and in real time localized translation and validate how each mRNA/protein is differentially regulated at each sub-cellular region during embryogenesis. We will then identify sub-cellular function of Vasa by manipulating its localization in the cell using advanced imaging techniques such as optogenetics and Chromophore- Assisted Light Inactivation (CALI). A biological event of localized mRNA translation as well as a DEAD- box RNA-helicase Vasa is highly conserved among various organisms and cells. The outcomes of this project will be thus useful to all researchers, especially those who study how sub-cellular level of mRNA and protein regulation impacts cellular regulation and development in any cells and organisms.

项目总结/摘要 mRNA定位于胚胎的特定细胞或细胞中的亚细胞区域， 空间控制的蛋白质生产。不同浓度的蛋白质导致不同的细胞命运 决定，身体模式和细胞功能在许多动物的发展。尤其是， 在胚胎细胞中，局部翻译的机制似乎是必不可少的，这些胚胎细胞很大， 快速的细胞分裂，需要立即输入特定的蛋白质，而不依赖于扩散动力学， 在细胞的其他地方翻译。事实上，有丝分裂纺锤体是一个亚细胞区域， 已报道mRNA存在于各种生物的卵/胚胎中。的机制或过程 然而，在纺锤体上的本地化翻译很少被确定。建议的研究重点 一个保守的死亡盒RNA解旋酶Vasa，它位于每个卵裂球的纺锤体上， 胚胎发生我们以前确定了Vasa在一般翻译调节中的功能， 功能障碍导致海胆胚胎中约80%的蛋白质合成减少和有丝分裂缺陷。 因此，我们假设在这些胚胎细胞中，Vasa有助于定位mRNA的过程， 主轴上的平移。为了验证我们的假设，使用海胆胚胎， Vasa在每个卵裂球的纺锤体上都有强有力的表达，我们将在体内和真实的时间内可视化 本地化翻译，并验证每个mRNA/蛋白质是如何在每个亚细胞 在胚胎发育过程中。然后，我们将通过操纵Vasa的亚细胞功能， 使用先进的成像技术，如光遗传学和生色团， 辅助光灭活（卡利）。一个生物学事件的本地化mRNA翻译以及死亡- box RNA-解旋酶Vasa在各种生物体和细胞中高度保守。这个结果 因此，该项目对所有研究人员都很有用，尤其是那些研究mRNA亚细胞水平如何的研究人员 蛋白质调节影响任何细胞和生物体中的细胞调节和发育。