Identifying protective omics profiles in centenarians and translating these into preventive and therapeutic strategies
确定百岁老人的保护性组学特征并将其转化为预防和治疗策略
基本信息
- 批准号:10449626
- 负责人:
- 金额:$ 33.78万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2019
- 资助国家:美国
- 起止时间:2019-09-15 至 2022-06-30
- 项目状态:已结题
- 来源:
- 关键词:Administrative SupplementAntibodiesBindingBiologicalBiological AssayBloodCentenarianChargeCollaborationsCollectionComputing MethodologiesCoupledDataDetectionEnrollmentFamily StudyFundingGenerationsGoalsHumanInfrastructureLabelLifeLongevityMainstreamingMass Spectrum AnalysisMeasurementMethylationParentsPerformancePhasePhenotypePopulationPreparationPreventivePropertyProteinsProteomeProteomicsProvinceQuality ControlReagentSamplingSensitivity and SpecificitySerumSpecificityTechnologyTherapeuticTranslatinganalysis pipelineaptamerbasecohortdata managementdesigndosageexperimental studyhead-to-head comparisonhealthy agingmetabolomicsmicrobiome researchmultiple omicsnext generation sequencingoffspringparent grantparent projectphenotypic dataprotein profilingrecruittranscriptomics
项目摘要
Assessing accuracy of protein measurements across multiple analytical platforms. Our NIA funded
project, UH2AG064704 “Identifying protective omics profiles in centenarians and translating these into
preventive and therapeutic strategies”, is a phased UH2/UH3 project. We are currently in the UH2 phase, in
which we achieve specific milestones to set the stage for and facilitate the conduct of the UH3 phase. Among
the major UH2 milestones, we are recruiting and enrolling 1,400 centenarians and their offspring, and we are
collecting their phenotype data, blood and fecal samples. In the UH3 phase, we will generate multi-omics
profiles of those samples and correlate them with extreme human longevity phenotypes. Other major
milestones of the UH2 phase include planning for the generation of omics data and developing analyses
pipelines that we will use in the UH3 phase of the project. We have made substantial progress toward
enrollment, data management, and plans for the majority of omics data and we also realized the importance of
using an accurate data generating platform that is well harmonized with those used by other studies of human
extreme longevity such as the Long Life Family Study and the Longevity Consortium. In our parent application,
we proposed to use the SOMAscan technology to generate serum proteomics. Alternative proteomics
approaches include labelled mass spectrometry and the Olink Explore platforms. These three mainstream
technologies have pros and cons in terms of required sample preparation, coverage of the human proteome,
accuracy of protein abundance assessment, and specificity of proteins detection and their performance has not
been compared in a comprehensive way. In this request for an administrative supplement we propose to
generate data that will inform the choice of the best proteomic platform to be used in the UH3 phase of the
project. We have the opportunity to join forces with other studies of human extreme longevity to compare mass
spectrometry, SOMAscan and Olink platforms using a well-designed spike-in experiment. We propose two
specific aims. Specific Aim 1: To generate SOMAlogic-based proteomic profiles of 250 samples that
represent triplicates of one control condition, and 6 pools including 8 different proteins spiked at 13 different
abundance, for a total of 3 + 3𝑥6𝑥13 = 237 samples. We will add an additional 13 blank samples for quality
control. Specific Aim 2: To conduct quality control analysis of the proteomic profiles generated using the
SOMAscan, Olink and Mass-spectrometry technologies. Cleaned and normalized data will be used to analyze
the dosage-based trajectories of protein abundance to detect proteins that change in the different pools and to
estimate sensitivity and specificity of protein detection of the SOMAscan, Olink and mass-spectrometry
technologies. The comparison of data generated with the Somascan technology versus alternative technology
will provide critical information to guide the selection of the best proteomic technology to use in the UH3 phase
of the parent project.
评估跨多个分析平台的蛋白质测量的准确性。我们的NIA资助
项目成果
期刊论文数量(5)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Protein cost minimization promotes the emergence of coenzyme redundancy.
- DOI:10.1073/pnas.2110787119
- 发表时间:2022-04-05
- 期刊:
- 影响因子:11.1
- 作者:
- 通讯作者:
Bayesian differential analysis of cell type proportions: opinion.
- DOI:10.3389/fgene.2023.1205499
- 发表时间:2023
- 期刊:
- 影响因子:3.7
- 作者:Karagiannis, Tanya T.;Monti, Stefano;Sebastiani, Paola
- 通讯作者:Sebastiani, Paola
Cell Type Diversity Statistic: An Entropy-Based Metric to Compare Overall Cell Type Composition Across Samples.
- DOI:10.3389/fgene.2022.855076
- 发表时间:2022
- 期刊:
- 影响因子:3.7
- 作者:
- 通讯作者:
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{{ truncateString('THOMAS T PERLS', 18)}}的其他基金
Identifying protective omics profiles in centenarians and translating these into preventive and therapeutic strategies
确定百岁老人的保护性组学特征并将其转化为预防和治疗策略
- 批准号:
10017131 - 财政年份:2019
- 资助金额:
$ 33.78万 - 项目类别:
Identifying protective omics profiles in centenarians and translating these into preventive and therapeutic strategies
确定百岁老人的保护性组学特征并将其转化为预防和治疗策略
- 批准号:
10678171 - 财政年份:2019
- 资助金额:
$ 33.78万 - 项目类别:
Protein Signatures of APOE2 and Cognitive Aging
APOE2 的蛋白质特征和认知衰老
- 批准号:
10451539 - 财政年份:2018
- 资助金额:
$ 33.78万 - 项目类别:
Protein Signatures of APOE2 and Cognitive Aging
APOE2 的蛋白质特征和认知衰老
- 批准号:
10219143 - 财政年份:2018
- 资助金额:
$ 33.78万 - 项目类别:
Protein Signatures of APOE2 and Cognitive Aging
APOE2 的蛋白质特征和认知衰老
- 批准号:
10408304 - 财政年份:2018
- 资助金额:
$ 33.78万 - 项目类别:
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