Real-time biosensor for mapping the function of the pancreas

用于绘制胰腺功能的实时生物传感器

• 批准号: 10455342
• 负责人: HYONGSOK Tom SOH
• 金额: $ 100万
• 依托单位: STANFORD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2018
• 资助国家: 美国
• 起止时间: 2018-03-15 至 2022-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10455342
• 关键词: Real; time; biosensor; mapping; function

Project Summary: The capability to manipulate pancreatic function through peripheral nerve stimulation offers exciting therapeutic potential, but will require a deeper understanding of underlying molecular biology as well as inter-organ signaling and communication. Such processes are difficult to study in vivo because they occur on time-scales of seconds to minutes—far faster than can be measured by conventional assays. The primary goal of this project is to develop real-time in vivo biosensors that can continuously measure the physiological concentrations of glucose, insulin and glucagon in specific areas of the pancreas of freely moving animals. Our biosensors will detect these three biomarkers with high sensitivity (pM~nM), spatial resolution (~10 m) and temporal resolution (~1 minute), and thereby offer unprecedented insights into the molecular biology of the pancreas. We will be building upon aptamer biosensor technology previously developed by our group, with which we have already demonstrated the first continuous measurement of small molecule drugs in vivo as well as closed-loop feedback control in live animals. This is a “platform” technology that can be readily adapted to measure a wide range of biomarkers by swapping the aptamer probes, as we have demonstrated previously. Upon successful development of this real-time biosensor for the pancreas, we will expand the range of targets to 20 other biomarkers, including neuromodulators and cytokines that play important roles in peripheral nerve stimulation. To make these biosensor systems available to other researchers, a startup company (RT Biotech) will refine the prototypes and produce them at low volumes for other groups within the SPARC program within 3 years.

项目概述：通过周围神经刺激操纵胰腺功能的能力提供

项目成果

Accelerated Electron Transfer in Nanostructured Electrodes Improves the Sensitivity of Electrochemical Biosensors.

• DOI: 10.1002/advs.202102495
• 发表时间: 2021-12
• 期刊: Advanced science (Weinheim, Baden-Wurttemberg, Germany)
• 作者: Fu K;Seo JW;Kesler V;Maganzini N;Wilson BD;Eisenstein M;Murmann B;Soh HT
• 通讯作者: Soh HT

High-Throughput Strategy for Enhancing Aptamer Performance across Different Environmental Conditions.

增强适配体在不同环境条件下性能的高通量策略。

• DOI: 10.1021/acssensors.2c02106
• 发表时间: 2023
• 期刊: ACS sensors
• 影响因子: 8.9
• 作者: Wan,Leighton;Yoshikawa,Alex;Eisenstein,Michael;Soh,HTom
• 通讯作者: Soh,HTom

Discovery of indole-modified aptamers for highly specific recognition of protein glycoforms.

• DOI: 10.1038/s41467-021-26933-1
• 发表时间: 2021-12-07
• 期刊: Nature communications
• 影响因子: 16.6
• 作者: Yoshikawa AM;Rangel A;Feagin T;Chun EM;Wan L;Li A;Moeckl L;Wu D;Eisenstein M;Pitteri S;Soh HT
• 通讯作者: Soh HT

A system for multiplexed selection of aptamers with exquisite specificity without counterselection.

• DOI: 10.1073/pnas.2119945119
• 发表时间: 2022-03-22
• 期刊: Proceedings of the National Academy of Sciences of the United States of America
• 影响因子: 11.1
• 作者: Yoshikawa AM;Wan L;Zheng L;Eisenstein M;Soh HT
• 通讯作者: Soh HT

Modular Aptamer Switches for the Continuous Optical Detection of Small-Molecule Analytes in Complex Media.

用于复杂介质中小分子分析物连续光学检测的模块化适体开关。

• DOI: 10.1002/adma.202304410
• 发表时间: 2024
• 期刊: Advanced materials (Deerfield Beach, Fla.)
• 作者: Hariri,AmaniA;Cartwright,AlyssaP;Dory,Constantin;Gidi,Yasser;Yee,Steven;Thompson,IanAP;Fu,KaiyuX;Yang,Kiyoul;Wu,Diana;Maganzini,Nicolò;Feagin,Trevor;Young,BrianE;Afshar,BehradHabib;Eisenstein,Michael;Digonnet,MichelJF
• 通讯作者: Digonnet,MichelJF