Telemetric Regenerative Bandage for Accelerating Wound Healing

用于加速伤口愈合的遥测再生绷带

• 批准号: 10663343
• 负责人: Guillermo Antonio Ameer
• 金额: $ 65.49万
• 依托单位: NORTHWESTERN UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-09-24 至 2026-07-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10663343
• 关键词: Acceleration; Acrylamides; Address; Adhesions; Amputation; Animal Model; Animals; Antioxidants; Bacterial Infections; Bandage; Biocompatible Materials; Blood Circulation; Body Temperature; Caring; Cells; Chronic; Citrates; Clinic; Clinical; Complications of Diabetes Mellitus; Copper; Dermal; Development; Devices; Diabetes Mellitus; Diabetic Foot Ulcer; Diabetic mouse; Digit structure; Disease; Endothelial Cells; Engineering; Exposure to; Family suidae; Feedback; Fibroblasts; Free Radicals; Gel; Goals; Health; Healthcare; High Prevalence; Hospitals; Human; Immobilization; Impaired healing; Impaired wound healing; Impairment; Implant; In Vitro; Incidence; Infection; Inflammation; Laminin; Left; Limb structure; Location; Lower Extremity; Managed Care; Measures; Medical Device; Metabolic syndrome; Metals; Miniature Swine; Monitor; Operative Surgical Procedures; Oxidative Stress; Patient-Focused Outcomes; Patients; Peptides; Personal Satisfaction; Physicians; Polyethylene Glycols; Population; Positioning Attribute; Prevalence; Process; Proliferating; Property; Research; Research Personnel; Safety; Sepsis; Shapes; Skin; Skin wound healing; Societies; Specific qualifier value; Sterile coverings; System; Telemetry; Temperature; Testing; Thick; Time; Tissues; Vascularization; Work; Wound Infection; angiogenesis; biomaterial compatibility; blood perfusion; clinically relevant; db/db mouse; diabetic; diabetic ulcer; effective therapy; electronic sensor; flexibility; healing; health care delivery; health care disparity; human disease; improved; innovation; keratinocyte; limb amputation; macromolecule; microsensor; migration; mouse model; nanoparticle; nanoparticle delivery; neovascularization; non-healing wounds; novel; outcome prediction; porcine model; prevent; regenerative; research clinical testing; scaffold; sensor; skin wound; therapy outcome; tissue regeneration; tool; wireless; wireless sensor; wound; wound bed; wound care; wound closure; wound dressing; wound healing

Summary Diabetic foot ulcers (DFUs) are a major complication of diabetes. These sores, if left untreated, can become infected and become a serious threat to the patient’s well being. Although the field of wound care management is well established, the effective treatment of chronic DFUs remains a challenge. The primary goal in the treatment of DFUs is for the wound to close as soon as possible and to do so in a durable way. However, prolonged inflammation, oxidative tissue damage, and impaired blood circulation in diabetic wounds delay the wound healing process, resulting in open, non-healing wounds that often lead to limb amputations. This proposal will address these problems by developing a versatile wound dressing that restores normal wound healing rates by reducing free radicals in the wound, providing a native-like scaffold for the cells to divide and migrate, and enhancing vascularization in the wound. Another problem is the inability to monitor the wound in real time after the patient leaves the hospital, leading to digit or limb amputations. We will address this problem by developing a wireless system that can monitor the temperature and pH of the wound in real time, parameters that have been shown to be indicators of infection. Therefore, the overall goal of this proposal is to develop a shape- conforming antioxidant dressing that upon exposure to body temperature transforms into a gel that promotes new tissue formation in diabetic wounds and a feedback system that involves tissue conforming sensors to monitor bacterial infection and/or lack of healing. Toward this goal, we have developed a novel macromolecule - poly (polyethylene glycol citrate-co-N isopropyl acrylamide) - that incorporates a laminin-derived peptide. This material, referred to as PPCN-A5G81, supports tissue regeneration and can conform to the unique shape and depth of a wound. As for wireless monitoring of the wound, we pioneered the development of flexible, stretchable electronic sensors that can be integrated with human skin or implanted into the body for continuous, non-invasive health monitoring and treatment of disease. We hypothesize that: 1) incorporating immobilized Cu2+ into PPCN-A5G81 will confer vasculoinductive properties that significantly increase PPCN-A5G81’s ability to restore normal healing rates of full thickness dermal wounds in diabetic mouse and swine models; and 2) conforming temperature and pH sensors are safe and can remotely provide real time information regarding blood perfusion and infection in dermal wounds in diabetic animals. The specific aims of this proposal are to: 1) fabricate a PPCN-based regenerative dressing with vasculoinductive, dermoconductive, and dermoinductive properties and investigate its safety and efficacy for healing full thickness wounds in diabetic mice and diabetic pigs with metabolic syndrome; and 2) fabricate and characterize telemetric wound feedback tissue-conforming sensors capable of measuring temperature and pH in infected and non-infected diabetic dermal wounds. Results from this research will contribute to the development of innovative clinical products that reduce amputation rates and improve patient outcome.

总结 糖尿病足溃疡（DFU）是糖尿病的主要并发症。这些疮，如果不治疗，可以成为 感染并对患者的健康构成严重威胁。虽然伤口护理管理领域 尽管已经确立，但慢性DFU的有效治疗仍然是一个挑战。的主要目标 DFU的治疗是尽快闭合伤口，并以持久的方式进行。然而，在这方面， 糖尿病伤口中的长期炎症、氧化性组织损伤和血液循环受损延迟了 伤口愈合过程中，导致开放，不愈合的伤口，往往导致截肢。这项建议 我将通过开发一种多功能伤口敷料来解决这些问题， 通过减少伤口中的自由基，为细胞分裂和迁移提供类似天然的支架， 增强伤口中的血管形成。另一个问题是在伤口愈合后不能真实的时间监测伤口。 病人离开医院，导致手指或四肢截肢。我们将通过开发 一个无线系统，可以监测温度和pH值的伤口在真实的时间，参数，已被 这是感染的迹象。因此，本提案的总体目标是制定一个形状- 一种适形抗氧化敷料，在暴露于体温时转化为凝胶， 促进糖尿病伤口中新组织的形成和涉及组织的反馈系统 符合传感器监测细菌感染和/或缺乏愈合。为了实现这一目标，我们 开发了一种新的高分子-聚（聚乙二醇柠檬酸酯-co-N异丙基丙烯酰胺）-， 掺入层粘连蛋白衍生肽。这种材料，称为PPCN-A5 G81，支持组织再生 并且可以符合伤口的独特形状和深度。至于伤口的无线监测，我们 开创了柔性、可拉伸电子传感器的开发，这些传感器可以与人体皮肤集成， 植入体内，用于连续的、非侵入性的健康监测和疾病治疗。我们假设 1）将固定化的Cu 2+并入PPCN-A5 G81将赋予血管诱导性质， 增加PPCN-A5 G81恢复糖尿病小鼠全层皮肤伤口正常愈合速率的能力 和猪模型; 2）符合温度和pH传感器是安全的，可以远程提供真实的时间 有关糖尿病动物皮肤伤口血液灌注和感染的信息。的具体目标 该建议是：1）制造具有血管诱导性，皮肤传导性， 和皮肤诱导特性，并研究其对糖尿病患者全层伤口愈合的安全性和有效性。 患有代谢综合征的小鼠和糖尿病猪;以及2）制造和表征遥测伤口反馈 能够测量感染和非感染糖尿病患者的温度和pH值的组织适应传感器 皮肤伤口这项研究的结果将有助于开发创新的临床产品， 降低截肢率并改善患者预后。

项目成果

Materials and Device Designs for Wireless Monitoring of Temperature and Thermal Transport Properties of Wound Beds during Healing.

用于无线监测愈合过程中伤口床温度和热传输特性的材料和设备设计。

• DOI: 10.1002/adhm.202302797
• 发表时间: 2024
• 期刊: Advanced healthcare materials
• 影响因子: 10
• 作者: Ryu,Hanjun;Song,JosephW;Luan,Haiwen;Sim,Youngmin;Kwak,SungSoo;Jang,Hokyung;Jo,YoungJin;Yoon,Hong-Joon;Jeong,Hyoyoung;Shin,Jaeho;Park,DoYun;Kwon,Kyeongha;Ameer,GuillermoAntonio;Rogers,JohnA
• 通讯作者: Rogers,JohnA