Telemetric Regenerative Bandage for Accelerating Wound Healing

用于加速伤口愈合的遥测再生绷带

• 批准号: 10663343
• 负责人: Guillermo Antonio Ameer
• 金额: $ 65.49万
• 依托单位: NORTHWESTERN UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-09-24 至 2026-07-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10663343
• 关键词: Acceleration; Acrylamides; Address; Adhesions; Amputation; Animal Model; Animals; Antioxidants; Bacterial Infections; Bandage; Biocompatible Materials; Blood Circulation; Body Temperature; Caring; Cells; Chronic; Citrates; Clinic; Clinical; Complications of Diabetes Mellitus; Copper; Dermal; Development; Devices; Diabetes Mellitus; Diabetic Foot Ulcer; Diabetic mouse; Digit structure; Disease; Endothelial Cells; Engineering; Exposure to; Family suidae; Feedback; Fibroblasts; Free Radicals; Gel; Goals; Health; Healthcare; High Prevalence; Hospitals; Human; Immobilization; Impaired healing; Impaired wound healing; Impairment; Implant; In Vitro; Incidence; Infection; Inflammation; Laminin; Left; Limb structure; Location; Lower Extremity; Managed Care; Measures; Medical Device; Metabolic syndrome; Metals; Miniature Swine; Monitor; Operative Surgical Procedures; Oxidative Stress; Patient-Focused Outcomes; Patients; Peptides; Personal Satisfaction; Physicians; Polyethylene Glycols; Population; Positioning Attribute; Prevalence; Process; Proliferating; Property; Research; Research Personnel; Safety; Sepsis; Shapes; Skin; Skin wound healing; Societies; Specific qualifier value; Sterile coverings; System; Telemetry; Temperature; Testing; Thick; Time; Tissues; Vascularization; Work; Wound Infection; angiogenesis; biomaterial compatibility; blood perfusion; clinically relevant; db/db mouse; diabetic; diabetic ulcer; effective therapy; electronic sensor; flexibility; healing; health care delivery; health care disparity; human disease; improved; innovation; keratinocyte; limb amputation; macromolecule; microsensor; migration; mouse model; nanoparticle; nanoparticle delivery; neovascularization; non-healing wounds; novel; outcome prediction; porcine model; prevent; regenerative; research clinical testing; scaffold; sensor; skin wound; therapy outcome; tissue regeneration; tool; wireless; wireless sensor; wound; wound bed; wound care; wound closure; wound dressing; wound healing

Summary Diabetic foot ulcers (DFUs) are a major complication of diabetes. These sores, if left untreated, can become infected and become a serious threat to the patient’s well being. Although the field of wound care management is well established, the effective treatment of chronic DFUs remains a challenge. The primary goal in the treatment of DFUs is for the wound to close as soon as possible and to do so in a durable way. However, prolonged inflammation, oxidative tissue damage, and impaired blood circulation in diabetic wounds delay the wound healing process, resulting in open, non-healing wounds that often lead to limb amputations. This proposal will address these problems by developing a versatile wound dressing that restores normal wound healing rates by reducing free radicals in the wound, providing a native-like scaffold for the cells to divide and migrate, and enhancing vascularization in the wound. Another problem is the inability to monitor the wound in real time after the patient leaves the hospital, leading to digit or limb amputations. We will address this problem by developing a wireless system that can monitor the temperature and pH of the wound in real time, parameters that have been shown to be indicators of infection. Therefore, the overall goal of this proposal is to develop a shape- conforming antioxidant dressing that upon exposure to body temperature transforms into a gel that promotes new tissue formation in diabetic wounds and a feedback system that involves tissue conforming sensors to monitor bacterial infection and/or lack of healing. Toward this goal, we have developed a novel macromolecule - poly (polyethylene glycol citrate-co-N isopropyl acrylamide) - that incorporates a laminin-derived peptide. This material, referred to as PPCN-A5G81, supports tissue regeneration and can conform to the unique shape and depth of a wound. As for wireless monitoring of the wound, we pioneered the development of flexible, stretchable electronic sensors that can be integrated with human skin or implanted into the body for continuous, non-invasive health monitoring and treatment of disease. We hypothesize that: 1) incorporating immobilized Cu2+ into PPCN-A5G81 will confer vasculoinductive properties that significantly increase PPCN-A5G81’s ability to restore normal healing rates of full thickness dermal wounds in diabetic mouse and swine models; and 2) conforming temperature and pH sensors are safe and can remotely provide real time information regarding blood perfusion and infection in dermal wounds in diabetic animals. The specific aims of this proposal are to: 1) fabricate a PPCN-based regenerative dressing with vasculoinductive, dermoconductive, and dermoinductive properties and investigate its safety and efficacy for healing full thickness wounds in diabetic mice and diabetic pigs with metabolic syndrome; and 2) fabricate and characterize telemetric wound feedback tissue-conforming sensors capable of measuring temperature and pH in infected and non-infected diabetic dermal wounds. Results from this research will contribute to the development of innovative clinical products that reduce amputation rates and improve patient outcome.

概括 糖尿病足溃疡（DFU）是糖尿病的主要并发症。这些疮如果不及时治疗，可能会变成 感染并严重威胁患者的健康。尽管伤口护理管理领域 虽然已得到充分证实，但慢性 DFU 的有效治疗仍然是一个挑战。的首要目标是 DFU 的治疗是让伤口尽快闭合并持久闭合。然而， 糖尿病伤口的长期炎症、氧化组织损伤和血液循环受损会延迟 伤口愈合过程，导致开放性、不愈合的伤口，通常导致肢体截肢。这个提议 将通过开发一种多功能伤口敷料来解决这些问题，该敷料可以恢复正常的伤口愈合率 通过减少伤口中的自由基，为细胞分裂和迁移提供类似天然的支架，以及 增强伤口的血管化。另一个问题是术后无法实时监测伤口 患者离开医院，导致手指或肢体截肢。我们将通过开发来解决这个问题 无线系统，可以实时监测伤口的温度和pH值，参数已被 表明是感染的指标。因此，该提案的总体目标是开发一个形状- 顺应性抗氧化敷料，在暴露于体温时会转化为凝胶， 促进糖尿病伤口中新组织的形成以及涉及组织的反馈系统 符合传感器来监测细菌感染和/或缺乏愈合。为了这个目标，我们有 开发了一种新型大分子——聚（聚乙二醇柠檬酸酯-co-N异丙基丙烯酰胺）—— 包含层粘连蛋白衍生肽。这种材料被称为 PPCN-A5G81，支持组织再生 并且可以符合伤口的独特形状和深度。至于伤口的无线监测，我们 率先开发了灵活、可拉伸的电子传感器，可以与人体皮肤或 植入体内进行持续、非侵入性的健康监测和疾病治疗。我们假设 1) 将固定化 Cu2+ 掺入 PPCN-A5G81 将赋予血管诱导特性，显着 提高 PPCN-A5G81 恢复糖尿病小鼠全层真皮伤口正常愈合率的能力 和猪模型； 2) 符合标准的温度和 pH 传感器是安全的，并且可以远程提供实时信息 有关糖尿病动物皮肤伤口的血液灌注和感染的信息。具体目标 该提案旨在：1）制造一种基于 PPCN 的再生敷料，具有血管诱导性、皮肤传导性、 和皮肤感应特性，并研究其治疗糖尿病全层伤口的安全性和有效性 患有代谢综合征的小鼠和糖尿病猪； 2) 制作并表征遥测伤口反馈 组织顺应性传感器能够测量感染和未感染糖尿病患者的温度和 pH 值 皮肤伤口。这项研究的结果将有助于开发创新的临床产品 降低截肢率并改善患者预后。

项目成果

Materials and Device Designs for Wireless Monitoring of Temperature and Thermal Transport Properties of Wound Beds during Healing.

用于无线监测愈合过程中伤口床温度和热传输特性的材料和设备设计。

• DOI: 10.1002/adhm.202302797
• 发表时间: 2024
• 期刊: Advanced healthcare materials
• 影响因子: 10
• 作者: Ryu,Hanjun;Song,JosephW;Luan,Haiwen;Sim,Youngmin;Kwak,SungSoo;Jang,Hokyung;Jo,YoungJin;Yoon,Hong-Joon;Jeong,Hyoyoung;Shin,Jaeho;Park,DoYun;Kwon,Kyeongha;Ameer,GuillermoAntonio;Rogers,JohnA
• 通讯作者: Rogers,JohnA