权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

Synaptic mechanisms of auditory thalamic cross-modal communication

听觉丘脑跨模式通讯的突触机制

基本信息

批准号：
10540232
负责人：
DANIEL A LLANO
金额：
$ 32.78万
依托单位：
UNIVERSITY OF ILLINOIS AT URBANA-CHAMPAIGN
依托单位国家：
美国
项目类别：
财政年份：
2021
资助国家：
美国
起止时间：
2021-01-01 至 2025-12-31
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10540232
关键词：
Acoustics Anatomy Area Attention Auditory Auditory area Auditory system Binding Brain Cell Nucleus Cells Characteristics Chemical Synapse Communication Communication impairment Data Disease Dorsal Dyslexia Electrical Synapse Electrophysiology (science)Environment Failure Genetic Goals Hearing Heart Image In Vitro Lasers Link Maps Measures Medial Medial geniculate body Mediating Modality Modeling Modernization Mus Neurons Optics Pathway interactions Physiological Population Preparation Process Property Rabies Resources Role Scanning Sensory Signal Transduction Slice Stimulus Stream Structure Synapses System Testing Thalamic Nuclei Thalamic structure Thinness Travel Virus Visual Work auditory thalamus autism spectrum disorder awake calcium indicator cognitive change computer studies genetic manipulation imaging approach in vivo inducible Cre lens cortex multimodality multisensory network architecture novel optical imaging pharmacologic response segregation sensory processing disorder sensory stimulus sensory system somatosensory

项目摘要

ABSTRACT Healthy subjects rapidly shift their attention in response to a dynamic sensory environment and changing cognitive demands. Failure to quickly shift perceptual resources between auditory and other modalities has been hypothesized to be a core deficit in both dyslexia and autism. Precisely how the brain shuttles information between sensory systems is not known, but recent work suggests that the thalamus contains circuits that have the capacity to rapidly transition between different sensory modalities. Specifically, the thalamic reticular nucleus (TRN), a thin shell of GABAergic neurons surrounding the thalamus, may serve as a link to allow communication between different areas of the sensory thalamus, a phenomenon we refer to as thalamic “cross- talk.” Based on recent data and our preliminary findings, we hypothesize that thalamo-TRN- thalamic circuits provide critical connections between thalamic nuclei and therefore permit rapid switching between auditory and other thalamocortical pathways. We propose to test this hypothesis using a novel combination of anatomical, chemogenetic, optical stimulation and optical imaging approaches in the mouse, using both slice and in vivo approaches. Specifically, we will determine which of multiple potential circuit pathways is/are used to permit auditory, visual and auditory thalamic nuclei to communicate with each other. Next, we will determine whether and how such thalamic cross-talk influences synaptic responses at the level of the auditory cortex. Finally, the impact of the TRN on cross-modal processing will be directly examined by optically modulating the TRN while imaging cortical responses to combined sensory stimulation in awake mice. Successful completion of this project will provide the first circuit-level characterization of the role of the TRN in communication between the auditory thalamus and other thalamic regions. In addition, this work will lay the groundwork for a greater understanding of how thalamoreticular systems break down in disorders of communication.

抽象的健康的受试者会迅速转移注意力以响应动态的感官环境，不断变化的认知需求。无法在听觉和听觉之间快速转移知觉资源其他方式被假设为阅读障碍和自闭症的核心缺陷。恰恰大脑如何在感觉系统之间传递信息尚不清楚，但最近的研究表明丘脑包含能够在不同状态之间快速转换的电路不同的感官方式。具体来说，丘脑网状核（TRN）是一个薄壳，丘脑周围的 GABA 能神经元可能充当沟通的纽带感觉丘脑的不同区域之间存在这种现象，我们将这种现象称为丘脑“交叉” 讲话。”根据最近的数据和我们的初步发现，我们假设 thalamo-TRN- 丘脑回路提供丘脑核之间的关键连接，因此允许快速听觉通路和其他丘脑皮质通路之间的切换。我们建议对此进行测试使用解剖学、化学遗传学、光刺激和光学的新颖组合的假设小鼠成像方法，使用切片和体内方法。具体来说，我们将确定多个潜在电路路径中的哪一个用于允许听觉、视觉和听觉丘脑核团相互沟通。接下来，我们将确定是否以及这种丘脑串扰如何影响听觉皮层水平的突触反应。最后，TRN 对跨模态处理的影响将通过光学直接检查调节 TRN，同时对清醒状态下组合感觉刺激的皮层反应进行成像老鼠。该项目的成功完成将提供该器件的第一个电路级表征 TRN 在听觉丘脑和其他丘脑区域之间的交流中的作用。在此外，这项工作将为更好地理解丘脑网状结构如何系统因沟通障碍而崩溃。