LungMap Phase II - Building a multidimensional map of developing human lung

LungMap 第二阶段 - 构建人类肺部发育的多维图

基本信息

批准号：
10672949
负责人：
Jeffrey A Whitsett
金额：
$ 89.73万
依托单位：
CINCINNATI CHILDRENS HOSP MED CTR
依托单位国家：
美国
项目类别：
财政年份：
2019
资助国家：
美国
起止时间：
2019-08-23 至 2024-07-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/10672949
关键词：
3-Dimensional ATAC-seq Acinus organ component Affect Agreement Algorithms Alveolar Alveolus Anatomy Atlases Bioinformatics Carbon Dioxide Cell Separation Cell model Cells Child Chromatin Code Collaborations Communities Complex Confocal Microscopy Congenital Disorders DNA Data Data Analyses Data Coordinating Center Data Display Defect Development Digestion Dimensions Disease Distal Doctor of Medicine Endothelium Epigenetic Process Fibroblasts Gases Gene Expression Generations Genetic Diseases Genetic Transcription Genomics Gland Goals Human Image Image Analysis Immune Immunofluorescence Immunologic In Situ In Situ Hybridization Individual Infant Intuition Link Lobule Location Lung Lung diseases Maps Mediating Molecular Morphogenesis Mus Normal tissue morphology Nuclear Nuclear RNA Online Systems Ontology Organ Oxygen Pathogenesis Patients Perinatal Phase Physiological Positioning Attribute Premature Infant Process Protein Isoforms Proteins Proteomics RNA RNA Probes RNA Splicing RNA analysis Regional Anatomy Research Resolution Role Science Signal Transduction Site Structure Structure of parenchyma of lung Submucosa Technology Tissue Procurements Tissues Trachea Visualization Work analysis pipeline analytical tool bioinformatics pipeline cell type central database confocal imaging data access data integration data visualization design diverse data droplet sequencing epigenomics experience genetic signature high resolution imaging high throughput technology human data human tissue image processing indexing insight lipidomics lung acinus structure lung development lung imaging member multidimensional data multiple omics new technology organ growth postnatal preservation programs pulmonary function reconstruction scaffold single nucleus RNA-sequencing tool transcriptome sequencing transcriptomics user-friendly web portal web services web site web-based tool

项目摘要

ABSTRACT Building a Multidimensional Map of Developing Human Lung – Jeffrey A. Whitsett, M.D. PI Our LungMAP2 project seeks to work in a collaborative consortium to develop operational pipelines to study human lung development from the saccular, alveolar stages of morphogenesis at single cell level of resolution. The project will integrate single cell transcriptomic, chromatin accessibility, 2D and 3D high-resolution confocal microscopy, immunofluorescence, multiprobe RNA in situ hybridization studies to create a detailed molecular map of the cells, cell-cell and cell scaffold interactions in their precise anatomic positions in the developing human lung. Our LungMAP2 Center will work in close concert with the Data Coordinating Center (DCC), other LungMAP2 Centers and the Human Tissue Procurement Core (HTPC) to obtain, annotate, preserve and store human tissues using optimal conditions for imaging and omics analyses. Our LungMAP2 project will produce a detailed structural expression atlas of multiple regions, niches in the human lung. We will develop and validate new technologies for tissue clearing, 3D reconstructions of high-resolution images, multi-probe RNA and IF analysis. We will develop new tissue digestion single cell and single nuclear RNA analyses for precise regional niches of the normal developing human lung, tissue from and in infants with rare congenital lung disorders. We will work in a Consortium to develop useful bioinformatic programs, modeling cell-cell, cell matrix interactions to define the signaling and transcriptional programs building and maintaining organ structure and function. Working with the DCC and other LungMAP2 Centers, our bioinformatics pipelines will enable data visualization, interrogation in user friendly web portals for sharing with members of the LungMAP2 Consortium, the DCC and for the research community in general.

抽象的构建发展中肺的多维图 - Jeffrey A. Whitsett，M.D。Pi 我们的Lungmap2项目旨在在协作联盟中工作，以开发运营管道研究人类肺发育从单细胞水平上形态发生的肺泡阶段的发育解决方案。该项目将整合单细胞转录组，染色质可及性，2D和3D 高分辨率共聚焦显微镜，免疫荧光，多种RNA原位杂交研究为了创建细胞的详细分子图，细胞细胞和细胞支架相互作用的精度在发展中的人肺中的解剖位置。我们的Lungmap2中心将与数据协调中心（DCC），其他LUNGMAP2中心和人体组织采购核心（HTPC）以最佳条件为成像和OMICS分析。我们的Lungmap2项目将产生详细的结构表达图集在多个地区，人类肺中的壁ni。我们将开发并验证组织的新技术清除，高分辨率图像，多探针RNA和IF分析的3D重建。我们将开发新的组织消化单细胞和单核RNA分析，以进行精确的区域壁ni 在患有罕见的先天性肺部疾病的婴儿和婴儿的正常发育中的人肺中。我们将在一个财团中工作，以开发有用的生物信息学程序，对细胞电池，细胞基质进行建模互动以定义信号传导和转录程序构建和维护器官结构和功能。与DCC和其他Lungmap2中心合作，我们的生物信息学管道将启用数据可视化，用户友好的Web门户中的询问以共享 Lungmap2财团，DCC和研究社区的成员。