Milk Analytics Core

牛奶分析核心

• 批准号: 10681304
• 负责人: Lars Bode
• 金额: $ 20.26万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN DIEGO
• 依托单位国家: 美国
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-09-10 至 2026-07-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10681304
• 关键词: Aliquot; Analytical Chemistry; Antibiotic Therapy; Antibiotics; Biological; Biological Assay; Biomedical Research; Breast Feeding; Breastfed infant; California; Cells; Childhood; Clinical; Clinical Sciences; Collaborations; Collection; Communities; Complex; Coupled; Data; Data Science; Detection; Disease; Fluorescence; Goals; Growth Factor; High Pressure Liquid Chromatography; Hormones; Human Characteristics; Human Milk; Immunoassay; Infant; Infant Development; Infant Health; Infant Mortality; Knowledge; Lactation; Lactose; Leukocyte L1 Antigen Complex; Lipids; Macronutrients Nutrition; Maternal and Child Health; Measurement; Measures; Microbe; Micronutrients; Milk; Mothers; Mus; Mutation; Near-Infrared Spectroscopy; Oligosaccharides; Outcome; Participant; Peptide Hydrolases; Peptides; Performance; Pregnancy; Proteins; Protocols documentation; Research; Research Personnel; Research Project Grants; Resources; Risk Reduction; Role; Safety; Sampling; Services; Technology; Therapeutic; Therapeutic Agents; Universities; Validation; Woman; analytical tool; assay development; biological heterogeneity; cytokine; design; experience; feeding; ghrelin; glucagon-like peptide 1; improved; infant morbidity/mortality; lipidomics; macromolecule; metabolomics; microbiome; microbiome analysis; milk fat globule; milk microbiome; operation; pharmacometrics; programs; response; sample collection; sialylation; skills; technology validation

ABSTRACT The University of California San Diego (UC San Diego) MPRINT Center of Excellence in Therapeutics (CET) involves a human milk (HM)-centered approach to investigate the role of maternal and pediatric therapeutics at intersections of the mother-milk-infant ‘triad’. Key among the overall goals of the MPRINT CET are to assess how maternal antibiotics impact HM composition and how HM components alter safety and efficacy of these important pediatric therapeutic agents. HM composition analysis, however, is not trivial. HM composition is highly dynamic and changes throughout lactation, indeed even throughout the course of a single feeding. HM is a complex matrix that includes cells and microbes as well as large molecule aggregates like the milk fat globule. HM also contains a unique set of molecules, the human milk oligosaccharides (HMOs), which represent the third most abundant component of HM after lactose and lipids. Thus, the dynamic, complex, and unique nature of HM requires specific collection protocols, assay development, and assay validation. With over 20 years of experience in HM research, the Milk Analytics Core (MAC) will provide overall guidance and advice to MPRINT CET investigators and staff. MAC will provide HM collection protocols and ready-to-ship sample collection kits to the Clinical and Data Science Projects (Aim 1a) and receive HM samples for composition analysis (Aim 1b). Depending on project needs, MAC offers macronutrient, oligosaccharide, and bioactive measurements as well as milk microbiome analysis, the latter in collaboration with the UC San Diego Microbiome Core, which operates independently of, but frequently in partnership with, our team. MAC will also provide mouse milk oligosaccharide and microbiome analyses to the Research Project team (Aim 1c), applying our detailed prior knowledge of how the St3gal4 mutation under study markedly depletes sialylated milk oligosaccharide content. Data generated from our analyses will be returned to the respective project teams for integration. Separate HM aliquots from the same samples will be passed directly on to the Pharmacometrics and Analytical Chemistry Core for antibiotic drug quantification, and that data will likewise be directly returned to the respective project teams. In addition to already established services, MAC will develop and validate new HM-specific assays by expanding the analyte portfolio of the existing multiplex immunoassay platform (Aim 2a) and by engaging and activating other UC San Diego researchers to apply and validate their unique technologies to HM research (Aim 2b). We envision MAC to become a comprehensive milk analytics core with knowledge, skills, and technology, serving as a resource to this and other MPRINT CETs and the biomedical research community at large. Together, we will be able to generate a better understanding how antibiotic treatment during pregnancy and lactation impacts the mother- milk-infant ‘triad’, inform and adjust precision in therapeutics, and ultimately improve maternal-child health.

抽象的 加州大学圣地亚哥分校 (UC San Diego) MPRINT 卓越治疗中心 (CET) 涉及以母乳 (HM) 为中心的方法来研究母乳和儿科治疗的作用 母乳婴儿“三位一体”的交叉点。 MPRINT CET 总体目标的关键是评估 母体抗生素如何影响 HM 成分以及 HM 成分如何改变这些药物的安全性和功效 重要的儿科治疗药物。然而，HM 成分分析并非微不足道。 HM 成分高度 在整个哺乳期，甚至在单次喂养过程中，动态和变化。 HM是一个 复杂的基质，包括细胞和微生物以及乳脂肪球等大分子聚集体。 HM 还含有一组独特的分子，即母乳低聚糖 (HMO)，它代表了第三种分子。 HM 中含量仅次于乳糖和脂质的成分。因此，HM 的动态性、复杂性和独特性 需要特定的收集方案、测定开发和测定验证。拥有超过20年的经验 在 HM 研究中，牛奶分析核心 (MAC) 将为 MPRINT CET 提供总体指导和建议 调查人员和工作人员。 MAC 将向 临床和数据科学项目（目标 1a）并接收 HM 样本进行成分分析（目标 1b）。 根据项目需求，MAC 还提供常量营养素、低聚糖和生物活性测量 作为牛奶微生物组分析，后者与加州大学圣地亚哥分校微生物组核心合作，该中心运营 独立于我们的团队，但经常与我们的团队合作。 MAC还将提供鼠奶低聚糖 和微生物组分析给研究项目团队（目标 1c），应用我们详细的先验知识 研究中的 St3gal4 突变显着降低了唾液酸化乳寡糖含量。生成的数据 我们的分析结果将返回给各自的项目团队进行整合。将 HM 等份从 相同的样品将直接传递至抗生素的药物计量和分析化学核心 药物定量，并且该数据同样将直接返回给各自的项目团队。此外 MAC 将通过扩展分析物来开发和验证新的 HM 特异性检测 现有多重免疫分析平台（目标 2a）的投资组合，并通过参与和激活其他 UC San 迭戈研究人员将他们独特的技术应用于 HM 研究（目标 2b）。我们设想MAC 成为具有知识、技能和技术的综合牛奶分析核心，作为资源 这个以及其他 MPRINT CET 和整个生物医学研究界。齐心协力，我们将能够 更好地了解怀孕和哺乳期间的抗生素治疗如何影响母亲 乳婴“三联体”，告知并调整治疗精度，最终改善母婴健康。