Center for Integrated Multi-modal and Multi-scale Nucleome Research
综合多模式和多尺度核组研究中心
基本信息
- 批准号:10678954
- 负责人:
- 金额:$ 130万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2020
- 资助国家:美国
- 起止时间:2020-09-24 至 2025-06-30
- 项目状态:未结题
- 来源:
- 关键词:3-DimensionalAcuteAffectArchitectureAtlasesBiologicalBiological AssayBrainBrain regionCRISPR interferenceCell NucleusCellsChromatinChromatin ModelingChromatin StructureCommunicationCommunitiesComplexDataDevelopmentDistalElementsEnhancersFree WillGene ExpressionGene Expression RegulationGenesGeneticGenetic TranscriptionGenomeGenome MappingsGenomicsGoalsHippocampusHumanHuman GenomeHypothalamic structureImageImaging DeviceIndividualKnowledgeMacacaMammalian CellMammalsMapsMeasurableMethodsModelingMonkeysMusNuclearPhysicsPhysiologicalPlayPolymersRegulator GenesResearchResearch PersonnelResearch Project GrantsResolutionRoleScientistSliceSocial BehaviorStructural ModelsStructureTechnologyTestingTissuesTranscriptional RegulationUntranslated RNAVirtual and Augmented realityVisualizationVisualization softwareanalytical toolcell cortexcell typecohesinembryonic stem cellepigenomeepigenomicsexperimental studygenomic datahigh resolution imagingimprovedinformatics toolinsightinventionlearning strategymammalian genomemultimodalitynanoscalenerve stem cellnervous system disorderneural circuitnew technologypredictive modelingprogramspromoterstatistical learningtooltranscriptomicsvirtual realityweb portal
项目摘要
The transcriptional regulatory sequences communicate with each other dynamically in the
3D nuclear space to direct cell type specific gene expression. Currently, a major barrier to
understanding the transcriptional regulatory programs is the lack of tools, models and maps
to explore the chromatin architecture in diverse cell types and physiological contexts. We
will address this pressing need by deploying transformative technologies to study the
chromatin architecture in mammalian cells at an unprecedented resolution and scale.
Specifically, we will generate navigable, cell-type-specific reference maps of chromatin
architecture in the mouse, macaque and human brains by integrating high resolution and
high throughput imaging and orthogonal single-cell-based genomic methods. We will also
dissect the role of chromatin architecture in gene regulation through a set of controlled
perturbation experiments in the mouse ES cells (ESC) and ESC-derived neural progenitor
cells (NPC). We will develop structural models of chromatin organization with advanced
polymer physics and statistical learning methods, and validate their predictive power in
embryonic stem cells and in ex vivo brain slices. Finally, we will make the reference maps,
analytical tools, visualization methods and structural models available to the broader
community. The proposed research project will dramatically transform our ability to analyze
the 4D Nucleome of complex tissues, and produce the much-needed maps, tools and
models for understanding the gene regulatory programs encoded in the linear genome
sequences.
转录调控序列在转录调控序列中动态地相互通信
3D核空间来指导细胞类型特异性基因的表达。目前,一个主要的障碍是
理解转录调控计划缺乏工具、模型和地图
探索不同细胞类型和生理环境下的染色质结构。我们
将通过部署变革性技术来研究
哺乳动物细胞中的染色质结构以前所未有的分辨率和规模。
具体来说,我们将生成可导航的、特定于细胞类型的染色质参考图
在小鼠、猕猴和人脑中通过集成高分辨率和
高通量成像和基于单细胞的正交基因组方法。我们还将
通过一组对照研究,剖析染色质结构在基因调控中的作用
小鼠胚胎干细胞及其神经前体细胞的扰动实验
细胞(NPC)。我们将开发先进的染色质组织结构模型
聚合物物理和统计学习方法,并验证它们的预测能力
胚胎干细胞和体外脑片。最后,我们将制作参考地图,
分析工具、可视化方法和结构模型可供更广泛的
社区。拟议的研究项目将极大地改变我们的分析能力
复杂组织的4D核组,并产生急需的地图、工具和
理解线性基因组中编码的基因调控程序的模型
序列。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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Catherine Dulac其他文献
Catherine Dulac的其他文献
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{{ truncateString('Catherine Dulac', 18)}}的其他基金
Odor trail tracking: a new paradigm to unveil algorithms and neural circuits underlying active sensation and continuous decision making
气味踪迹追踪:揭示主动感觉和持续决策背后的算法和神经回路的新范例
- 批准号:
10524245 - 财政年份:2022
- 资助金额:
$ 130万 - 项目类别:
Molecular and genetic dissection of brain circuits controlling fever
控制发烧的脑回路的分子和遗传解剖
- 批准号:
10373051 - 财政年份:2020
- 资助金额:
$ 130万 - 项目类别:
Systems-Level and in Situ Transcriptomics Deconstruction of Neural Circuits Underlying Sensorimotor Transformation in an Innate Behavior
先天行为中感觉运动转化的神经回路的系统级和原位转录组学解构
- 批准号:
10709855 - 财政年份:2020
- 资助金额:
$ 130万 - 项目类别:
Molecular and genetic dissection of brain circuits controlling fever
控制发烧的脑回路的分子和遗传解剖
- 批准号:
10589104 - 财政年份:2020
- 资助金额:
$ 130万 - 项目类别:
Center for Integrated Multi-modal and Multi-scale Nucleome Research
综合多模式和多尺度核组研究中心
- 批准号:
10269034 - 财政年份:2020
- 资助金额:
$ 130万 - 项目类别:
Center for Integrated Multi-modal and Multi-scale Nucleome Research
综合多模式和多尺度核组研究中心
- 批准号:
10458025 - 财政年份:2020
- 资助金额:
$ 130万 - 项目类别:
Microcircuits underlying murine parental behavior
小鼠父母行为背后的微电路
- 批准号:
10461107 - 财政年份:2015
- 资助金额:
$ 130万 - 项目类别:
Microcircuits underlying murine parental behavior
小鼠父母行为背后的微电路
- 批准号:
10227959 - 财政年份:2015
- 资助金额:
$ 130万 - 项目类别:
Microcircuits underlying murine parental behavior
小鼠父母行为背后的微电路
- 批准号:
10674853 - 财政年份:2015
- 资助金额:
$ 130万 - 项目类别:
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