Development of a handheld probe for confocal microscopy and Raman spectroscopy*

开发用于共焦显微镜和拉曼光谱的手持式探头*

• 批准号: 7843690
• 负责人: Anita Mahadevan-Jansen
• 金额: $ 27.9万
• 依托单位: VANDERBILT UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-07-20 至 2012-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7843690
• 关键词: Academic Medical Centers; Achievement; American Cancer Society; Appearance; Arts; Benchmarking; Benign; Biochemical; Biochemistry; Biopsy; Bladder; Cancer Detection; Categories; Characteristics; Clinic; Clinical; Clinical Research; Computer software; Confocal Microscopy; Custom; Data; Dermatologist; Detection; Development; Devices; Diagnosis; Diagnostic; Diagnostic Procedure; Differential Diagnosis; Dimensions; Disease; Disease Management; Distal; Epithelium; Excision; Future; Generations; Goals; Gold; Healthcare; Histology; Human; Image; Imaging Device; Inflammation; Lesion; Location; Malignant - descriptor; Malignant Neoplasms; Malignant Squamous Cell; Measurement; Measures; Medicine; Modeling; Morphology; Nevus; Normal tissue morphology; Optics; Organ; Ovary; Pathology; Patient Care; Patients; Pattern; Performance; Physicians' Offices; Principal Investigator; Process; Prostate; Raman Spectrum Analysis; Research Personnel; Resolution; Scanning; Screening procedure; Self-Examination; Site; Skin; Skin Cancer; Skin Tissue; Staging; Structure; System; Technical Expertise; Techniques; Technology; Testing; Therapeutic; Time; Tissues; Visual; Weight; Work; base; body system; cost effectiveness; design; disease diagnosis; heterodyning; imaging probe; improved; in vitro testing; in vivo; instrument; interest; new technology; novel; programs; prototype; skin lesion; spectroscopic imaging; success; tool

DESCRIPTION (provided by applicant): The American Cancer Society recommends that the best way to find skin cancers early is to recognize changes in existing skin lesions or the appearance of new lesions by regular self-examination. One way to facilitate this routine examination of skin lesions would be the availability of an easy-to-use, non-invasive device that can scan the body in a relatively short time. Such a device could be utilized through the general physician's office or the dermatologist's office. Thus a novel tool that provides spatial, structural as well as biochemical information about tissue disease state is proposed directed initially towards skin cancer management. This revised proposal seeks to develop a handheld combined confocal Raman spectroscopy and confocal imaging device that, can provide real-time, morphologic as well as biochemical information of skin lesions, potentially identifying these lesions as normal, benign and malignant for diagnosis of the disease. Raman spectroscopy is a purely biochemical technique and yields little information about the tissue microstructure. Confocal imaging could provide spatial as well as structural information that together with confocal Raman spectroscopy could yield information about tissue structure as well as biochemistry allowing for complete diagnosis. Thus this proposal is focused on the development of a clinical, confocal Raman instrument with confocal imaging as well as bright-field imaging capability for the differential diagnosis of skin lesions by providing real-time, automated, non-intrusive spectral as well as spatial information about the tissue biochemistry as well as structure. The steps needed to implement such a device will be pursued independently. The specific aims of the proposed project are as follows; Specific Aim (1): Develop and test a compact handheld Raman probe with video imaging capability. Specific Aim (2) Develop and test a handheld confocal imaging device at video rate: Specific Aim (3): Develop and test an integrated handheld confocal Raman/scanning confocal imaging handheld device. Specific Aim (4): Test signatures of normal and malignant skin tissues in vivo. Specific Aim (5): Understand the basis and dynamics of the malignancy associated changes observed in malignant tissues in regions away from the disease. The pilot clinical studies planned in this proposal will be conducted at the Vanderbilt University Medical Center and the Vanderbilt Clinic. The development of the proposed work will have a significant impact on health care by providing the potential for complete disease management with a single compact, sensitive detection tool. With the success of this technique, this multi-modal instrument can be applied to other organ sites such as the prostate and ovary as well as other diseases impacting a broader field in medicine.

描述(申请人提供)：美国癌症协会建议，早期发现皮肤癌的最好方法是通过定期自我检查来识别现有皮损的变化或新皮损的出现。促进这种皮肤损伤常规检查的一种方法是提供一种简单易用的非侵入性设备，可以在相对较短的时间内扫描身体。这样的设备可以通过全科医生的办公室或皮肤科医生的办公室使用。因此，提出了一种新的工具，它提供关于组织疾病状态的空间、结构以及生化信息，最初是针对皮肤癌的管理。这项修订建议旨在开发一种手持式结合共聚焦拉曼光谱和共聚焦成像设备，可以提供皮肤病变的实时、形态和生化信息，潜在地识别这些病变的正常、良性和恶性，以用于疾病的诊断。拉曼光谱是一种纯粹的生化技术，几乎没有关于组织微观结构的信息。共聚焦成像可以提供空间和结构信息，与共聚焦拉曼光谱一起可以产生关于组织结构和生化的信息，从而实现完整的诊断。因此，这项建议的重点是开发一种临床共聚焦拉曼仪器，具有共焦成像和明视场成像能力，通过提供关于组织生化和结构的实时、自动、非侵入性的光谱和空间信息来鉴别诊断皮肤病变。实施这样一个装置所需的步骤将独立进行。该项目的具体目标如下：具体目标(1)：研制并测试一种具有视频成像能力的紧凑型手持拉曼探头。具体目标(2)开发和测试视频率手持共焦成像设备：特定目标(3)：开发和测试集成的手持共焦拉曼/扫描共焦成像设备。具体目的(4)：检测体内正常皮肤组织和恶性皮肤组织的特征。具体目标(5)：了解在远离疾病的区域观察到的恶性肿瘤相关变化的基础和动力学。该提案中计划的试点临床研究将在范德比尔特大学医学中心和范德比尔特诊所进行。拟议工作的开展将通过单一紧凑、灵敏的检测工具提供全面疾病管理的可能性，从而对卫生保健产生重大影响。随着这项技术的成功，这种多模式仪器可以应用于其他器官部位，如前列腺和卵巢，以及影响医学更广泛领域的其他疾病。

项目成果

A clinical instrument for combined raman spectroscopy-optical coherence tomography of skin cancers.

用于皮肤癌联合拉曼光谱-光学相干断层扫描的临床仪器。

• DOI: 10.1002/lsm.21041
• 发表时间: 2011
• 期刊: Lasers in surgery and medicine
• 影响因子: 2.4
• 作者: Patil,ChetanA;Kirshnamoorthi,Harish;Ellis,DarrelL;vanLeeuwen,TonG;Mahadevan-Jansen,Anita
• 通讯作者: Mahadevan-Jansen,Anita

MEMS-based handheld confocal microscope for in-vivo skin imaging.

• DOI: 10.1364/oe.18.003805
• 发表时间: 2010-02-15
• 期刊: Optics express
• 影响因子: 3.8
• 作者: Arrasmith CL;Dickensheets DL;Mahadevan-Jansen A
• 通讯作者: Mahadevan-Jansen A