Acquisition of a CytoFLEX LX Flow Cytometer to characterize extracellular vesicle (EV) formation and multiparametric flow cytometry assays

购买 CytoFLEX LX 流式细胞仪来表征细胞外囊泡 (EV) 的形成和多参数流式细胞术分析

• 批准号: 10797180
• 负责人: MELINDA K DUNCAN
• 金额: $ 24.77万
• 依托单位: UNIVERSITY OF DELAWARE
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-09-30 至 2025-04-30
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10797180
• 关键词: Address; Area; Bacteria; Basic Science; Biological Assay; Biology; Bone Marrow Cells; Cardiovascular Diseases; Cell Communication; Cells; Clinical; Color; Complement component C4; Dedications; Delaware; Detection; Drug Delivery Systems; Flow Cytometry; Goals; Health; Human; Institution; Lasers; Life; Malignant Neoplasms; Modernization; Mus; Neurosciences; Noise; Organism; Research; Research Personnel; Resolution; Risk; Signal Transduction; Technology; Therapeutic; Universities; Virus-like particle; detector; equipment acquisition; exosome; extracellular vesicles; fluorescence activated cell sorter device; fungus; instrument; nanoparticle drug; nutrition; pancreatic cancer cells; parent grant; particle; single cell analysis; stem cell biology; vesicular release

Project Summary/Abstract The aim of this project is to acquire a Beckman CytoFLEX LX flow cytometer for small particle and multicolor flow cytometry. The University of Delaware Flow Cytometry Core has two fluorescence-activated cell sorters (BD FACS Aria Fusions), but does not have a dedicated, modern analytical flow cytometer for multicolor flow cytometry panels and small particle (~80 nm) analyses. The existing BD FACS Aria Fusion can resolve only up to ~300-1000 nm particles, a range that does not achieve the lower limits of small particle and EV biology. Acquiring an instrument with FSC detectors specializing in small particle detection will make it possible to use flow cytometry to study the rapidly growing field of research on extracellular vesicles (EV) and exosomes. It will open up new avenues of research for UDEL investigators studying EV release in human and mouse bone marrow cells, pancreatic cancer cells, bacteria, fungi as well as other small particles, such as virus-like particles and nanoparticles for drug delivery. With a complement of 4 active lasers (and detectors) similar to the current Aria Fusions at the core labs, existing users can efficiently transition to the CytoFLEX LX for dedicated analysis purposes. Additionally, new and existing users will also benefit from the CytoFLEX LX’s highly sensitive avalanche photodiode (APDs) technology which provide a greater sensitivity and better resolution of signal-to- noise ratios compared to existing PMT technology inherent to other flow cytometers. Furthermore, it will greatly expand the UD flow cytometry core’s capabilities to conduct multiparametric single cell analyses with multicolor flow cytometry panels to reveal clinical and translationally relevant findings.

项目总结/摘要 本项目的目的是获得一台Beckman CytoFLEX LX流式细胞仪，用于小颗粒和微球的检测。 流式细胞仪特拉华州大学流式细胞仪核心有两个荧光激活细胞分选仪（BD FACS Aria Fusions），但没有专用的现代分析流式细胞仪用于流式细胞术。 流式细胞术板和小颗粒（~80 nm）分析。现有的BD FACS Aria Fusion只能分辨 至~300-1000 nm颗粒，该范围未达到小颗粒和EV生物学的下限。 购买一台配备专门用于小颗粒检测的FSC检测器的仪器， 流式细胞术研究细胞外囊泡（EV）和外来体的快速增长的研究领域。它将 为UDEL研究人员研究EV在人类和小鼠骨髓中的释放开辟了新的研究途径 细胞、胰腺癌细胞、细菌、真菌以及其它小颗粒，如病毒样颗粒和 用于药物输送的纳米颗粒。与目前的Aria类似，配有4个有源激光器（和探测器） 在核心实验室进行融合，现有用户可以高效地过渡到CytoFLEX LX进行专用分析 目的此外，新用户和现有用户也将受益于CytoFLEX LX的高度敏感性， 雪崩光电二极管（APD）技术，提供了更高的灵敏度和更好的信号分辨率， 与其他流式细胞仪固有的现有PMT技术相比，此外，它将大大 扩展UD流式细胞仪核心的功能，以使用流式细胞仪进行多参数单细胞分析 流式细胞术面板，以揭示临床和诊断相关的发现。