Bridging SenNet and HuBMAP through spatial omics of the human intestine

通过人体肠道的空间组学桥接 SenNet 和 HuBMAP

• 批准号: 10895625
• 负责人: ANDREW B. NIXON
• 金额: $ 20万
• 依托单位: DUKE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-09-30 至 2026-08-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10895625
• 关键词: Address; Age; Aging; Agreement; Area; Atlases; Biochemical; Bioinformatics; Biological; Blood; Cell Aging; Cell model; Cells; Cellular Structures; Clinical; Clinical Trials; Collaborations; Colon; Communication; Computer software; Consultations; Critical Care; Data; Data Analyses; Data Collection; Development; Disease; Evaluation; Faculty; Future; Goals; Heart; Heart Transplantation; Heterogeneity; Human; Human BioMolecular Atlas Program; Immune; Immunologics; Immunophenotyping; Improve Access; Institution; Intestines; Investigation; Knowledge; Leadership; Lung; Lung Transplantation; Maps; Methods; Mission; Molecular; Muscle; Normal Cell; Normal tissue morphology; Organ; Organoids; Peripheral Blood Mononuclear Cell; Phase; Phenotype; Problem Solving; Process; Protocols documentation; Research; Resolution; Scientist; Skin; Specimen; Testing; Therapeutic; Tissues; Translational Research; Transplantation Surgery; United States National Institutes of Health; Universities; age related; biobank; biological adaptation to stress; data integration; data sharing; deep learning algorithm; demographics; design; dimensional analysis; disability; healthy aging; human tissue; interoperability; medical schools; multimodality; normal aging; preservation; prospective; repaired; resilience; senescence; single cell technology; skills; targeted treatment; tissue mapping

ABSTRACT –SCENT– Overall Cellular senescence is a stress-response, as well as a critical component of cell fate during development, repair, resilience and normal aging. Deepening and broadening our investigations into cellular senescence in normal condition will advance our knowledge of healthy aging as well as age-related disabilities, thereby leading to integrated and inclusive approaches to Gero-Protection and Gero-Therapeutics. A comprehensive, high- resolution, Atlas of cellular senescence in various human tissues based on multimodal and multidimensional analyses is required to address this need. The Duke Senescent Cell Evaluations in Normal Tissues (SCENT) U54 Tissue Mapping Center (TMC) will leverage the broad and world-leading expertise of Duke University School of Medicine faculty to contribute to the overall goal of mapping senescent cells at single cell resolution in normal human tissues of across age-span and demographics. We will take advantage of Duke's broad expertise in many areas, including pulmonary critical care, lung and heart transplant surgery, translational research, and bioinformatics, to collect high-quality normal tissue specimens for biobanking, multimodal, high-resolution analysis, and data integration to fully characterize cellular senescence in human organs and tissues. The Duke SCENT TMC will be organized around 4 Cores as required by SenNet: The Administrative Core (AC) of this TCM will provide guidance and leadership to the other cores and communicate with NIH Staff and the SenNet Consortium during the set-up phase to agree on common protocols. The Biospecimen Core will establish best practices for prospective acquisition, preservation and processing of diverse, high-quality normal healthy human tissues and associated biofluids. The Biological Analysis Core will deliver state-of-the-art profiling, led by a team of highly accomplished research scientists who have successfully utilized these specific platforms in conjunction with previous and/or current senescent analyses. The Data Analysis Core (DAC) will manage, process and analyze the data generated to profile senescent cell signatures and construct senescent cell tissue maps, as well as collaborate with the Administrative Core to coordinate sharing of data, methods and pipelines with other SenNet institutions.

抽象 - 风味 - 总体 细胞感应是一种应力反应，也是在发育，修复过程中的细胞脂肪的关键成分 弹性和正常衰老。加深和扩大我们对正常细胞感应的研究 病情将提高我们对健康衰老以及与年龄相关的残疾的了解，从而导致 Gero保护和Gero治疗药的综合和包容性方法。全面，高 分辨率，基于多模式和多维的各种人组织中细胞感应的地图 需要分析以满足这一需求。正常组织中的公爵衰老细胞评估（气味） U54组织映射中心（TMC）将利用杜克大学学校的广泛而世界领先的专业知识 医学教师的总体目标是在正常情况下以单细胞分辨率绘制感觉细胞的整体目标 跨年龄和人口统计学的人体组织。我们将利用杜克在许多人中的广泛专业知识 包括肺重症监护，肺和心脏移植手术，翻译研究以及 生物信息学，收集高质量的正常组织标本，用于生物群，多模式，高分辨率 分析以及数据整合以完全表征人体器官和组织中的细胞感应。 Duke Scent TMC将按照Sennet的要求组织约4个核心： TCM的行政核心（AC）将向其他核心提供指导和领导 在设置阶段与NIH员工和Sennet联盟进行沟通，以同意共同的协议。 Biospecimen Core将建立前瞻性获取，保存和处理的最佳实践 潜水员，高质量的正常健康人体组织和相关的生物流体。 生物分析核心将提供最新的分析，由一支高度成就的团队领导 成功利用这些特定平台以及以前和/或的研究科学家 当前的感觉分析。 数据分析核心（DAC）将管理，处理和分析生成的档案中的数据 签名和构建感觉细胞组织图，以及与行政核心合作 与其他Sennet机构协调数据，方法和管道的共享。