Preclinical Evaluation Of Neutralizing Antibodies Elicit

中和抗体的临床前评估引发

• 批准号: 6684230
• 负责人: John R Mascola
• 金额: --
• 依托单位: NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/6684230
• 关键词: AIDS vaccines; Rodentias; flow cytometry; human immunodeficiency virus 1; immunization; lymphocyte; method development; monocyte; neutralizing antibody; tissue /cell culture; vaccine development

The ability to make accurate and precise measurements of antibody-mediated virus neutralization is important for the evaluation of mechanisms of neutralization and for the assessment of antibody responses elicited by immunization. For HIV-1, the use of primary virus isolates and human target cells, such as PBMC, is an in vitro assay system that is believed to measure physiologically relevant virus neutralization. Using this assay, preclinical antibody responses generated by vaccine candidates were screened and measured.

对抗体介导的病毒中和作用进行精确测量的能力对于评估中和作用机制和评估免疫引起的抗体反应非常重要。对于HIV-1，使用原代病毒分离物和人类靶细胞，如PBMC，是一种体外测定系统，被认为可以测量生理上相关的病毒中和。使用这种方法，筛选和测量候选疫苗产生的临床前抗体反应。