DROSOPHILA LONGEVITY ASSURANCE GENES

这个项目的长期目标是识别直接 影响人类衰老和健康的基因，称为长寿保障基因 (滞后)。以果蝇黑腹果蝇为模型 用于识别LAG的有机体，因为它的寿命短，容易 培养，以及许多可以应用的遗传和分子工具 到它的书房。果蝇和人类衰老的相似之处 细胞和分子水平表明，在 果蝇可能有功能上的同源物。选择了7个候选LAG 根据几个标准从已知的果蝇基因中分离出来。这些基因是 参与应激反应、DNA修复和能量 存储，并且每个都有已知的人类功能同源物。基因 将通过实验测试对果蝇寿命的影响 在转基因果蝇中增加和降低它们的活性。在……里面 此外，还将进行基因筛查，以识别新的果蝇 滞后。这些屏幕将利用新开发的P型转置 元素作为诱变剂，称为PdL。PDL创建主导条件 突变，这应该会极大地方便识别， 果蝇新基因LAG的鉴定与克隆。

The long term goal of this project is to identify genes which directly affect human aging and health span, called Longevity Assurance Genes (LAGs). The fruit fly Drosophila melanogaster is used as a model organism for identifying LAGs because of its short life span, ease of culture, and the many genetic and molecular tools which can be applied to its study. The similarities between aging in Drosophila and humans at the cellular and molecular level suggest that LAGs identified in Drosophila may have functional homologs. 7 candidate LAGs were selected from known Drosophila genes based on several criteria. The genes are involved are involved in stress responses, DNA repair and energy storage, and each has known functional homologs in humans. The genes will be tested for effects on Drosophila life span by experimentally increasing and decreasing their activity in transgenic flies. In addition, genetic screens will be performed to identify novel Drosophila LAGs. These screens will utilize a newly-developed P-type transposable element as a mutagen, called PdL. PdL creates dominant conditional mutations, which should greatly facilitate the identification, characterization and cloning of novel Drosophila LAGs.