3D Multiscale Reference Atlas for Human Skin

人体皮肤 3D 多尺度参考图集

• 批准号: 10708873
• 负责人: Sashank K Reddy
• 金额: $ 50万
• 依托单位: JOHNS HOPKINS UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-09-26 至 2024-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10708873
• 关键词: 3-Dimensional; Age; Aging; Architecture; Atlases; Biological Models; Biology; Cells; Chromatin; Classification; Communication; Data Analyses; Data Science Core; Dermal; Environment; Epigenetic Process; Extracellular Matrix; Fibroblasts; Genetic Transcription; Genotype; Hair follicle structure; Human; Image; Imaging technology; Individual; Location; Longevity; Machine Learning; Maps; Molecular; Molecular Profiling; Natural History; Organ; Pattern; Phenotype; Race; Reproducibility; Research Personnel; Resolution; Skin; Standardization; Structure; Systems Analysis; Tissues; Underrepresented Populations; Universities; Variant; Visualization software; analysis pipeline; cell community; cell type; data submission; image guided; multi-scale atlas; multiple omics; pathogen; repaired; response; senescence; sex; stem cells; tool; transcriptomics; user-friendly

The scientific focus of the proposed 3D Multiscale Reference Atlas for Human Skin is to develop the first comprehensive, three-dimensional set of integrated cellular and molecular maps of human skin across age, sex, race and skin location. Skin is the largest human organ, serving as a critical barrier between the external environment and internal tissues, yet no comprehensive map exists for the three-dimensional architecture of this organ at a cellular level, nor is there a comprehensive map of the molecular signatures defining this architecture across the body and across lifespan. Here, we will integrate our recently developed machine learning-guided image analysis system CODA with single-cell and spatial multi-omic analyses to produce high-content reference maps. The integrated Skin Atlas will allow researchers to interrogate the skin in its entire three-dimensional architecture, and/or focus on individual structures such as the vasculature or hair follicles, or individual cells. We will overlay genotypic and phenotypic states to enable, for example, examination of the overlap between senescent cells and stem cells, or the correlation between extracellular matrix (ECM) content and the diversity of dermal fibroblasts. Because we will include diverse and often underrepresented populations, our Multiscale Atlas will also provide a broader view of skin biology, filling in critical missing information. Finally, by looking across lifespan, the Atlas will provide a view of architectural, cellular, and molecular alterations in skin with age, establishing the natural history of normal biology for this critical organ. In addition to its native value for understanding skin itself, we anticipate that our Skin Atlas will be a powerful hypothesis generating tool for biologists studying the many questions for which skin is an ideal model system – homeostatic renewal, repair, senescence, aging, and pathogen response.

提出的人体皮肤三维多尺度参考地图集的科学重点是开发第一个