Developmental Project Competition

B.3.B.发展项目。在第一年，有五个存款保障计划获选资助 中西部RCE行动。假设存款保障计划的预算为每年500,000元的直接费用， 中西部RCE将发布另一个RFA，以在第一年选择1-2个额外的资助方案。 在第1年请求最高资助额(100,000美元直接成本)的DPS不能 在供资的第二年继续进行。预计DP研究将为 编制新的资金提案，提交给中西部区域经济委员会(RPS)、NIAID、能源部、 国防部或卫生部。中西部RCE科学家Dominique Missiakas将寻找炭疽杆菌 PA、LF或EF分泌缺陷的突变体，并分析其毒力特性 疾病动物模型中的变异。DP2“中西部RCE科学家格雷格·波兰将分离 痘苗病毒衍生的加工肽，来自第I类，人类白细胞抗原A2分子的结合槽 痘苗病毒感染树突状细胞并研究其物理和生化特性 多肽表位。DP3：中西部RCE科学家小詹姆斯·贝克将(I)记录该能力和 纳米乳剂灭活痘病毒的动力学，(II)确定纳米乳剂是否灭活 痘苗病毒具有免疫原性，以及(Iii)确定纳米乳剂是否可以预防或减弱 小鼠实验性痘苗病毒感染。DP4：中西部RCE科学家Paula Traktman将 使用RNAi技术和表位分析来鉴定那些对病毒至关重要的痘病毒基因 复制和萌芽。中西部RCE科学家Thomas Zahrt和Dara Frank将 构建图拉氏拟青霉转座子文库并寻找不能表达转座子的突变体 在体外巨噬细胞中存活。这些突变体将在小鼠的气溶胶模型中进行分析。 在发病机制上存在缺陷。在每年的秋季和春季，咨询委员会将发布请求 适用于要求100,000美元或更少的新建议书来取代DP计划的应用程序 以及最多两年的资助(不超过10万美元)。

B.3.b. Developmental Projects. Five DPs have been selected for funding during the first year of Midwestem RCE operation. Assuming a budget of $500,000 direct costs per year for DPs, the Midwestem RCE will post another RFA to select 1-2 additional proposals for funding in year 1. Those DPs that requested the maximum amount of funding in year 1 ($100,000 direct costs) cannot be continued in the second year of funding. It is expected that DP research generates data for the preparation of new funding proposals, submitted either to the Midwestem RCE (RPs), to NIAID, DOE, DOD or DOHS. DPI" Midwestem RCE scientist Dominique Missiakas will search for B. anthracis mutants that are defective for the secretion of PA, LF, or EF and analyze the virulence properties of the variants in an animal model of disease. DP2" Midwestem RCE scientist Greg Poland will isolate Vaccinia virus-derived, processed peptides from the binding grooves of class I, HLA-A2 molecules of Vaccinia virus-infected dendritic cells and study the physical and biochemical attributes of these peptide epitopes. DP3: Midwestem RCE scientist James Baker Jr. will (i) document the ability and kinetics of nanoemulsion inactivation of poxviruses, (ii) determine whether nanoemulsion inactivated Vaccinia virus is immunogenic and (iii) determine whether nanoemulsion can prevent or attenuate experimental Vaccinia virus infection in mice. DP4: Midwestem RCE scientist Paula Traktman will use RNAi technology and epitagging to identify those poxvirus genes that are essential for virus replication and budding. DP5" Midwestem RCE scientists Thomas Zahrt and Dara Frank will construct a transposon library in F. tularensis strain LVS and search for mutants that are unable to survive in macrophages in vitro. These mutants will then be analyzed in a murine model of aerosol infection for defects in pathogenesis. In the fall and spring of each year, the AC will post Requests For Applications to replace DP programs with new proposals that can request either $100,000 or less and up to two years of funding (not to exceed $100,000).