Annual Rhythms of Energy Balance and Behavior

能量平衡和行为的年度节奏

• 批准号: 7351342
• 负责人: JOHN G DARK
• 金额: $ 33.25万
• 依托单位: UNIVERSITY OF CALIFORNIA BERKELEY
• 依托单位国家: 美国
• 财政年份: 1996
• 资助国家: 美国
• 起止时间: 1996-09-16 至 2010-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7351342
• 关键词: Affect; Agonist; Animals; Behavior; Body Temperature; Body fat; Brown Fat; Condition; Daily; Eating; Energy Metabolism; Food; Goals; Health; Human; Hypothalamic structure; Ibotenic Acid; Injection of therapeutic agent; Lesion; Mammals; Maps; Medial; Modeling; Neurons; Neuropeptide Y Receptor; Neuropeptides; Neurosecretory Systems; Peptides; Peripheral; Phodopus sungorus; Play; Preoptic Areas; Regulation; Reproduction; Research; Role; Siberian Hamster; Site; Structure of nucleus infundibularis hypothalami; Temperature; Time; energy balance; experience; natural hypothermia; neuromechanism; neuropeptide Y; neurotoxic; paraventricular nucleus; prevent; receptor; research study

DESCRIPTION (provided by applicant): The overall goal is to characterize the role that body fat reserves and hypothalamic peptide mechanisms, especially neuropeptide Y (NPY), play in controlling daily torpor. Daily torpor is an extreme form of temperature regulation that evolved to help animals contend with limited food availability by uniquely reducing energy expenditure. The central and peripheral neural mechanisms integrating overall energy balance and torpor will be explored. Annual rhythms of food intake and body mass, reproduction, and daily torpor are well documented in the Siberian hamster (Phodopus sungorus), the model species in all experiments. Specific aims include: 1) contrasting the effects of direct intra-medial preoptic area (MPOA) injections of NPY on body temperature, specifically its ability to induce torpor and affect the timing of changes in brown adipose tissue temperature vs. body temperature; 2) developing an anatomical understanding of the distribution of NPY receptor subtypes in the Siberian hamster hypothalamus; 3) comparing and contrasting NPY receptor agonist activation and antagonist inhibition of NPY receptor sites in the MPOA on torpor induction; 4) evaluating the necessity of the MPOA for both induced (NPY & 2DG) and natural torpor using neuron-specific neurotoxic lesions; 5) comparing and contrasting effects of direct injections of NPY and NPY receptor agonists into the paraventricular nucleus of the hypothalamus on interscapular brown adipose tissue temperature (and body temperature).

描述（由申请人提供）：总体目标是描述身体脂肪储备和下丘脑肽机制，特别是神经肽 Y (NPY) 在控制日常麻木状态中所发挥的作用。每日麻木是一种极端的温度调节形式，它的进化是为了通过独特地减少能量消耗来帮助动物应对有限的食物供应。将探索整合整体能量平衡和麻木状态的中枢和外周神经机制。西伯利亚仓鼠（Phodopus sungorus）是所有实验中的模型物种，其每年的食物摄入量、体重、繁殖和日常麻木节律都有详细记录。具体目标包括：1) 对比直接内侧视前区 (MPOA) 注射 NPY 对体温的影响，特别是其诱导麻木状态并影响棕色脂肪组织温度与体温变化时间的能力； 2) 深入了解西伯利亚仓鼠下丘脑中 NPY 受体亚型的分布； 3) 比较和对比 MPOA 中 NPY 受体位点的 NPY 受体激动剂激活和拮抗剂抑制对麻木诱导的影响； 4) 使用神经元特异性神经毒性损伤评估 MPOA 对于诱导（NPY 和 2DG）和自然麻木的必要性； 5)比较和对比下丘脑室旁核直接注射NPY和NPY受体激动剂对肩胛间棕色脂肪组织温度(和体温)的影响。