BLOOD-DERIVED DENDRITIC CELLS AND EPITHELIAL CELLS

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目及 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 本研究探讨树突状细胞（DC）与气道上皮细胞共培养的细胞生物学。 目的是确定不同的DC亚型是否预测对环境颗粒物的免疫应答（Th 1与Th 2）。 将离体培养来自血液和支气管镜检查的原代细胞。 这项高度创新的研究应提供重要的基本信息。 研究人员将建立一个新的实验室系统，其中同种异体a）血液单核细胞或B）血液CD 34阳性脐带血细胞在双室装置中培养，该装置允许这些细胞在存在不同细胞因子、FIT 3配体、环境空气颗粒物的未指定混合物的情况下接触分化的呼吸道上皮细胞，血液单核细胞或CD 34阳性细胞将通过未指定的手段进行监测，以确定上皮细胞和细胞混合物所暴露的刺激物可能影响这些细胞分化的各种可能方式。 上皮细胞对前体细胞分化为未成熟和成熟DC的影响是重要的，值得研究的问题。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. This study investigates the cell biology of dendritic cells (DC) in co-culture with airway epithelial cells. The objective is to determine if different DC subtypes predict the immune response (Th1 vs. Th2) to ambient particulate matter. Primary cells derived from blood and bronchoscopy will be cultured ex vivo. This highly innovative study should provide important basic information. The investigators will set up a new laboratory system in which allogeneic a) blood monocytes or b) blood CD34-positive cord-blood cells are cultured in a two-chamber apparatus that permits these cells to contact differentiated respiratory epithelial cells in the presence of unspecified mixtures of different cytokines, FIT3 ligand, ambient air particulate matter, etc. The blood monocytes or CD34-positive cells will be monitored by unspecified means to determine the various possible ways that the differentiation of these cells might be influenced by the epithelial cells and the stimuli to which the cell mixtures are exposed. The issue of the influence of epithelial cells on the differentiation of precursor cells into immature and mature DC is important and worthy of study.